Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,419,837	G→T	S122R (AGC→AGA)	ppiA ←	peptidyl‑prolyl cis‑trans isomerase A (rotamase A)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,419,837	0	G	T	100.0%	174.6 / NA	46	S122R (AGC→AGA)	ppiA	peptidyl‑prolyl cis‑trans isomerase A (rotamase A)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/22);  total (24/22)

GTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTT  >  REL606/3419765‑3419909                                                                         |                                                                         gTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTg                                                                       >  2:1331927/1‑76 (MQ=255) gTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTg                                                                       >  1:883324/1‑76 (MQ=255) gTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGCGGCTCTg                                                                       >  1:352974/1‑76 (MQ=255)     ccGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCtt                                                                   <  2:1394899/76‑1 (MQ=255)     ccGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCtt                                                                   <  2:1225990/76‑1 (MQ=255)     ccGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCtt                                                                   <  2:1141376/76‑1 (MQ=255)      cGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCttt                                                                  >  1:583787/1‑76 (MQ=255)        aaaTCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGt                                                                >  2:1025930/1‑76 (MQ=255)          aTCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCa                                                              <  1:859953/76‑1 (MQ=255)          aTCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCa                                                              <  1:1665476/76‑1 (MQ=255)           tCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGGGGCTCTGTCTTTGTCAg                                                             >  1:1489036/1‑76 (MQ=255)               gCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGt                                                         <  2:1527646/76‑1 (MQ=255)                cTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTa                                                        <  1:994116/76‑1 (MQ=255)                cTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTa                                                        <  2:475120/76‑1 (MQ=255)                 tGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTTTTTGTCAGCGGTAc                                                       >  1:813907/1‑76 (MQ=255)                  gACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTAcg                                                      >  2:1062053/1‑76 (MQ=255)                  gACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTAcg                                                      <  1:791600/76‑1 (MQ=255)                   aCCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTAcgc                                                     >  1:907930/1‑76 (MQ=255)                   aCCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTAcgc                                                     >  1:1078089/1‑76 (MQ=255)                    ccATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTAcgcg                                                    >  2:614598/1‑76 (MQ=255)                     cATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACgcgc                                                   >  1:1033531/1‑76 (MQ=255)                       tGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCa                                                 >  1:361077/1‑76 (MQ=255)                           cAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATcgc                                             <  2:1190675/76‑1 (MQ=255)                            aaggaaggCGTTATCGGCAACGTTGATAAAGAACTGGCTGGGGGCTCTGTCTTTGTCAGCGGTACGCGCCATcgcg                                            >  2:1366824/1‑76 (MQ=255)                              tgaaggCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGAGGTACGCGCCCTCGCGAt                                          >  2:1514153/2‑76 (MQ=255)                              ggaaggCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGAt                                          >  2:215913/1‑76 (MQ=255)                                aaggCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATgg                                        >  1:79715/1‑76 (MQ=255)                                 aggCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGt                                       <  1:1381927/76‑1 (MQ=255)                                     gTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCa                                   >  1:1163009/1‑76 (MQ=255)                                      ttATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTTTCAGCGGTACGCGCCATCGCGATGGTGGCag                                  >  2:1416207/1‑75 (MQ=255)                                      ttATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCAc                                  >  1:1296521/1‑76 (MQ=255)                                       tATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCAcg                                 <  1:553517/76‑1 (MQ=255)                                       tATCGGCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCAcg                                 <  1:172612/76‑1 (MQ=255)                                          cGGCAACGTTGATAAAGAACGGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCgt                              <  1:1279697/76‑1 (MQ=255)                                           ggCAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCgtg                             <  2:1061211/76‑1 (MQ=255)                                             cAACGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGtt                           >  2:1471669/1‑76 (MQ=255)                                              aaCGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTg                          <  2:1323986/76‑1 (MQ=255)                                              aaCGTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTg                          <  2:172715/76‑1 (MQ=255)                                                 gTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTgcgc                       <  2:751765/76‑1 (MQ=255)                                                 gTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTgcgc                       <  2:1249985/76‑1 (MQ=255)                                                 gTTGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTgcgc                       <  2:195501/76‑1 (MQ=255)                                                  ttGATAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTgcgcg                      >  1:1035255/1‑75 (MQ=255)                                                      tAAAGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGcc                  >  1:306188/1‑76 (MQ=255)                                                       aaaGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCg                 >  1:1168043/1‑76 (MQ=255)                                                       aaaGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTGCACAGGCCg                 >  2:402843/1‑76 (MQ=255)                                                        aaGAACTGGCTGGTGGCTCTGTCTTTGTCAGCGGCACGCGCCATCGCGATGGAGCCACGCGTGTTGCgccggccgg                >  2:1115016/1‑75 (MQ=255)                                                         aGAACTGGCTGGTGGGCCTGTCTTTGTCAGGGGGATCGGCCAGCGCGGTGGGGGCACGCGAGATGCGCAGGGCggc               >  2:1521602/1‑74 (MQ=255)                                                           aaCTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTAt             >  1:1316064/1‑76 (MQ=255)                                                           aaCTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTAt             >  2:657357/1‑76 (MQ=255)                                                            aCTGGCTGGTGGCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGGCACGCGTGTTGCGCAGGCCGTTATc            <  2:1020056/76‑1 (MQ=255)                                                                   ggtggCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCa     <  1:128880/76‑1 (MQ=255)                                                                      ggCTCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCAttt  >  1:894225/1‑76 (MQ=255)                                                                         |                                                                         GTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGCGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTT  >  REL606/3419765‑3419909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑