Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,124,062	Δ1 bp	coding (159/1536 nt)	mdoG →	glucan biosynthesis protein, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,124,062	0	T	.	100.0%	337.0 / NA	67	coding (159/1536 nt)	mdoG	glucan biosynthesis protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (29/38);  total (29/38)

AAGCAAGCTCAATCTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGAC  >  REL606/1123991‑1124130                                                                        |                                                                     aagcaagcTCAATCTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGaa                                                                 <  1:1735692/76‑1 (MQ=255)    caagcTCAATCTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATa                                                              <  1:520655/76‑1 (MQ=255)     aagcTCAATCTTTAGCTGGGAAAGGCTACGAGACGCCGAAAAGCAACTTGCCCTNCGTTTTCCGCGA‑ATGAAATAc                                                             >  2:290861/1‑76 (MQ=255)      agcTCAATCTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATAcg                                                            <  2:394813/76‑1 (MQ=255)      agcTCAATCTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATAcg                                                            <  1:1309610/76‑1 (MQ=255)          cAATCTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGa                                                        >  2:271263/1‑76 (MQ=255)          cAATCTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGa                                                        >  2:373734/1‑76 (MQ=255)          cAATCTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGa                                                        <  1:1274429/76‑1 (MQ=255)          cAATCTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGa                                                        >  2:944074/1‑76 (MQ=255)            aTCTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACt                                                      <  1:1785039/76‑1 (MQ=255)            aTCTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACt                                                      <  1:239122/76‑1 (MQ=255)             tCTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTa                                                     <  2:391130/76‑1 (MQ=255)             tCTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTa                                                     <  2:1168725/76‑1 (MQ=255)              cTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTAt                                                    <  1:1672062/76‑1 (MQ=255)               tttAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATc                                                   >  1:396014/1‑76 (MQ=255)                ttAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATca                                                  >  2:109374/1‑76 (MQ=255)                 tAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATcag                                                 >  1:215314/1‑76 (MQ=255)                  aGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATcaga                                                >  1:458602/1‑75 (MQ=255)                   gCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATcagca                                               >  2:1276146/1‑76 (MQ=255)                    cTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATcagcag                                              >  2:270436/1‑76 (MQ=255)                     tGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGa                                             <  2:542841/76‑1 (MQ=255)                     tGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGa                                             >  1:972834/1‑76 (MQ=255)                     tGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGa                                             >  2:398176/1‑76 (MQ=255)                      gggAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGAt                                            <  1:351399/76‑1 (MQ=255)                        gAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATcc                                          >  2:762865/1‑76 (MQ=255)                          aaGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAg                                        >  2:414086/1‑76 (MQ=255)                          aaGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAg                                        <  1:1002405/76‑1 (MQ=255)                              cTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTa                                    >  2:1395320/1‑76 (MQ=255)                               tACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTaa                                   >  1:293425/1‑76 (MQ=255)                                aCGAGACGCCCAAAAGCAACTTGCTCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAAt                                  >  1:1168704/1‑76 (MQ=255)                                aCGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAAt                                  <  2:965552/76‑1 (MQ=255)                                aCGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAAt                                  <  1:1250555/76‑1 (MQ=255)                                 cGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATc                                 <  1:1587612/76‑1 (MQ=255)                                  gagaCGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCa                                <  2:627604/76‑1 (MQ=255)                                  gagaCGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCa                                <  2:1434841/76‑1 (MQ=255)                                  gagaCGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCa                                <  2:1270632/76‑1 (MQ=255)                                   agaCGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCAt                               <  2:570856/76‑1 (MQ=255)                                    gaCGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATg                              <  1:239371/76‑1 (MQ=255)                                       gCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACa                           <  1:1345638/76‑1 (MQ=255)                                         ccAAAAGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACaaa                         <  1:1174196/76‑1 (MQ=255)                                             aaGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGt                     <  1:1383920/76‑1 (MQ=255)                                             aaGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGt                     <  2:813284/76‑1 (MQ=255)                                              aGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTa                    <  2:658630/76‑1 (MQ=255)                                              aGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTa                    <  1:538788/76‑1 (MQ=255)                                              aGCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTa                    <  1:1137278/76‑1 (MQ=255)                                               gCAACTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTAc                   <  1:398176/76‑1 (MQ=255)                                                 aaCTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTg                 >  2:574674/1‑76 (MQ=255)                                                  aCTTGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTgg                >  1:1161976/1‑76 (MQ=255)                                                    ttGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGaa              >  2:977082/1‑76 (MQ=255)                                                    ttGCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGaa              >  1:737599/1‑76 (MQ=255)                                                     tGCCCTCCGTTTTCCGCGA‑ATGAAACACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAAc             >  2:1426662/1‑76 (MQ=255)                                                      gCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACa            <  1:1116351/76‑1 (MQ=255)                                                      gCCCTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGACCCAGTTTAATCATGACAAAGCGTACTGGAACa            <  1:1153969/76‑1 (MQ=255)                                                       cccTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACaa           <  1:1573919/76‑1 (MQ=255)                                                        ccTCCGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAAt          >  2:154984/1‑76 (MQ=255)                                                           ccGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTg       <  1:417101/76‑1 (MQ=255)                                                           ccGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTg       <  1:1534036/76‑1 (MQ=255)                                                           ccGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTg       >  2:233785/1‑76 (MQ=255)                                                           ccGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTg       <  2:227212/76‑1 (MQ=255)                                                            cGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGa      <  2:1156487/76‑1 (MQ=255)                                                            cGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGa      >  2:1470441/1‑76 (MQ=255)                                                            cGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGa      >  2:1748643/1‑76 (MQ=255)                                                            cGTTTTCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGa      <  2:968158/76‑1 (MQ=255)                                                              ttttCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAg    <  1:979681/76‑1 (MQ=255)                                                               tttCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGa   >  1:249240/1‑76 (MQ=255)                                                                ttCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGAc  >  1:1568761/1‑76 (MQ=255)                                                                ttCCGCGA‑ATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGAc  >  1:1568739/1‑76 (MQ=255)                                                                        |                                                                     AAGCAAGCTCAATCTTTAGCTGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGAC  >  REL606/1123991‑1124130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑