Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,484,1050AG28.0% 97.2 / 20.9 50G55G (GGA→GGG) ydcUpredicted spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (6/30);  new base G (14/0);  total (20/30)
Fisher's exact test for biased strand distribution p-value = 4.13e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGT  >  REL606/1484032‑1484175
                                                                         |                                                                      
tACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtt                                                                      <  2:233634/76‑1 (MQ=255)
  ctgctgCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtttt                                                                    <  2:1173872/76‑1 (MQ=255)
    gctgctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtat                                                                  <  2:1228371/76‑1 (MQ=255)
     ctgctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAttttttc                                                                 >  1:1590014/1‑73 (MQ=255)
        ctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACtt                                                              <  2:935355/76‑1 (MQ=255)
         ttGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTTCttt                                                             >  2:1114893/1‑76 (MQ=255)
         ttGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACttt                                                             <  2:1373678/76‑1 (MQ=255)
          tGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACtttt                                                            >  1:1802276/1‑76 (MQ=255)
           ggCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACttttt                                                           >  1:1484468/1‑75 (MQ=255)
               cACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGa                                                       <  2:143904/76‑1 (MQ=255)
                 cTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGAGTTTTACTTTTTACGaat                                                     >  2:1664056/1‑74 (MQ=255)
                  tAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTAATTTTTTCGAttt                                                    >  1:473882/1‑76 (MQ=255)
                   aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTACTTTTTACGAtttt                                                   >  1:837018/1‑75 (MQ=255)
                   aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTc                                                   <  1:297252/76‑1 (MQ=255)
                    aTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTGCGAtttta                                                  >  2:952448/1‑74 (MQ=255)
                    aTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGAttttc                                                  >  1:1691579/1‑74 (MQ=255)
                      gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCAcc                                                <  2:1339141/76‑1 (MQ=255)
                      gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCAcc                                                <  2:69839/76‑1 (MQ=255)
                          tttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGt                                            <  1:491625/76‑1 (MQ=255)
                            tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTGTCGATTTCACCCTGTCg                                          >  2:1769384/1‑76 (MQ=255)
                            tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCg                                          <  1:85189/76‑1 (MQ=255)
                              gCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGt                                        <  1:1517704/76‑1 (MQ=255)
                               cATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTa                                       <  1:891304/76‑1 (MQ=255)
                                aTTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTaa                                      <  2:117445/76‑1 (MQ=255)
                                  tGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACg                                    <  1:1576475/76‑1 (MQ=255)
                                      tATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTTTACTTTTGAAGATTTCACCATGTCGGGAACGCCgg                                >  2:764094/1‑76 (MQ=255)
                                         ttCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCCTGTCGGGAACGCCgggaa                             >  2:1267838/1‑73 (MQ=255)
                                         ttCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATTCTTTTTACGATTTTACCATGTCGGTAACGCCGGCAc                             >  2:1048541/1‑76 (MQ=255)
                                             gCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTTACGATTTCACCATGTCGGTAACGCCGGAACTGAc                         >  1:1345724/1‑76 (MQ=255)
                                             gCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGAc                         <  1:639858/76‑1 (MQ=255)
                                                ngctgctGACACTGTTATGGCAGGGGTTTTATACTTTTTACGATTTTACCATGTCGGTAACCCCGGCACTGACGCt                      >  1:1779457/2‑76 (MQ=255)
                                                cGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCt                      <  2:925319/76‑1 (MQ=255)
                                                 gctgctGACACTGTTATGGCAGGGATTTTTTAATTTTGCCGCTTTCACCCTGTTGGTAAAGCCGGCACTGACGCTg                     >  1:988816/1‑76 (MQ=255)
                                                   tgctgACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCACCATGTCGGGAACGCCGGAACTGACGCTggg                   >  1:133336/1‑75 (MQ=255)
                                                   tgctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGc                   <  1:1123122/76‑1 (MQ=255)
                                                     ctgACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGa                 >  2:782138/1‑76 (MQ=255)
                                                     ctgACACTGTTATGGCAGGGATTTTTTACTTTTGACGATTTCACCATGTCGGTAACGCCGGGACTGACGCTGGCGa                 >  2:541381/1‑76 (MQ=255)
                                                     ctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGa                 >  1:116067/1‑76 (MQ=255)
                                                        acacTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAata              <  1:1531652/76‑1 (MQ=255)
                                                         cacTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAatat             <  2:1506003/76‑1 (MQ=255)
                                                          acTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATc            <  2:1152628/76‑1 (MQ=255)
                                                           cTGTTATGGCAGGGGTTTTTTATTTTTGACGATTTCACCATGTCGGTAACGCCCGGACTGACGCCGGGGGatattc           >  2:1036959/1‑74 (MQ=255)
                                                            tGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCg          <  1:378110/76‑1 (MQ=255)
                                                             gTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGt         <  2:1613803/76‑1 (MQ=255)
                                                             gTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGt         <  2:763595/76‑1 (MQ=255)
                                                              ttATGGCAGGGGTTTTTTACTTTTGACGATTTCACCATGTCGGGAACGCCGGAACTGACGCTGGGGGATATCCGTg        >  1:322585/1‑76 (MQ=255)
                                                              ttATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTg        <  2:1352464/76‑1 (MQ=255)
                                                              ttATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTg        <  2:386342/76‑1 (MQ=255)
                                                                aTGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTgcg      >  2:1681176/1‑76 (MQ=255)
                                                                aTGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTgcg      <  2:1311942/76‑1 (MQ=255)
                                                                 tGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTgcgc     >  1:850425/1‑76 (MQ=255)
                                                                  ggCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCt    <  1:1314801/76‑1 (MQ=255)
                                                                  ggCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCt    <  1:461153/76‑1 (MQ=255)
                                                                  ggCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCt    <  2:1500323/76‑1 (MQ=255)
                                                                   gCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTg   >  1:659085/1‑76 (MQ=255)
                                                                    cAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGt  >  2:166901/1‑76 (MQ=255)
                                                                         |                                                                      
TACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGT  >  REL606/1484032‑1484175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑