Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,332,132	T→C	intergenic (+115/‑95)	topA → / → cysB	DNA topoisomerase I/DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,332,132	0	T	C	100.0%	337.8 / NA	77	intergenic (+115/‑95)	topA/cysB	DNA topoisomerase I/DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/39);  total (38/39)

GGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATG  >  REL606/1332062‑1332204                                                                       |                                                                         ggCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTa                                                                     <  2:82981/76‑1 (MQ=255)  gCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATANATGATATAGTGGTTACAGTTAg                                                                    <  2:248493/76‑1 (MQ=255)  gCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAg                                                                    <  2:1667858/76‑1 (MQ=255)      ttttttATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCAcc                                                                >  1:1189516/1‑76 (MQ=255)       tttttATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCt                                                               >  2:1490325/1‑76 (MQ=255)        ttttATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCtt                                                              >  1:89277/1‑76 (MQ=255)        ttttATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCtt                                                              <  2:1030316/76‑1 (MQ=255)          ttATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCtttt                                                            <  2:1024871/76‑1 (MQ=255)          ttATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCtttt                                                            >  2:155434/1‑76 (MQ=255)          ttATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCtttt                                                            >  1:222237/1‑76 (MQ=255)            aTTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCtttttt                                                          <  1:710735/76‑1 (MQ=255)            aTTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCtttttt                                                          <  1:1480538/76‑1 (MQ=255)             ttGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTNGTTACAGTTAGCACCTTTTtta                                                         <  2:242467/76‑1 (MQ=255)             ttGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTtta                                                         >  1:658012/1‑76 (MQ=255)             ttGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTtta                                                         <  2:422744/76‑1 (MQ=255)             ttGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTtta                                                         >  2:1509586/1‑76 (MQ=255)             ttGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTtta                                                         >  2:1665096/1‑76 (MQ=255)               gCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTttatt                                                       <  1:1271235/76‑1 (MQ=255)                cATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTttatta                                                      <  2:763597/76‑1 (MQ=255)                cATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTttatta                                                      <  2:1517067/76‑1 (MQ=255)                  tattatTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTttattatt                                                    <  1:1211495/76‑1 (MQ=255)                   attattTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTttattatta                                                   <  1:1753608/76‑1 (MQ=255)                   attattTTTTCTTCACACCTANACACTAAGGCTATAAAGGATATAGTGGTTACAGTTAGCACCTTTTttattatta                                                   <  2:291139/76‑1 (MQ=255)                    ttattTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTaa                                                  <  1:940010/76‑1 (MQ=255)                    ttattTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTaa                                                  >  1:504279/1‑76 (MQ=255)                    ttattTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTaa                                                  <  1:859938/76‑1 (MQ=255)                    ttattTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTaa                                                  <  1:263736/76‑1 (MQ=255)                    ttattTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTaa                                                  <  1:1575580/76‑1 (MQ=255)                      attTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAAt                                                >  1:712052/1‑76 (MQ=255)                      attTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAAt                                                <  1:1001377/76‑1 (MQ=255)                       ttttttCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATc                                               >  2:10357/1‑76 (MQ=255)                        tttttCTTCACACCTATACACTNAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCg                                              >  2:254192/1‑76 (MQ=255)                        tttttCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCg                                              >  1:575433/1‑76 (MQ=255)                         ttttCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGt                                             >  1:558637/1‑76 (MQ=255)                          tttCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTa                                            >  1:1617327/1‑76 (MQ=255)                          tttCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTa                                            >  2:577570/1‑76 (MQ=255)                          tttCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTa                                            <  2:666598/76‑1 (MQ=255)                           ttcttcACACCTATACACTAAGNCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTAt                                           >  2:248423/1‑76 (MQ=255)                           ttcttcACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTAt                                           <  1:1657623/76‑1 (MQ=255)                            tcttcACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTAtt                                          <  2:1377326/76‑1 (MQ=255)                            tcttcACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTAtt                                          <  2:1377317/76‑1 (MQ=255)                             cttcACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTa                                         >  1:106682/1‑76 (MQ=255)                             cttcACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTa                                         >  1:933930/1‑76 (MQ=255)                             cttcACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTa                                         >  1:1156373/1‑76 (MQ=255)                              ttcACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAg                                        >  2:1562136/1‑76 (MQ=255)                               tcACACCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGt                                       >  2:994747/1‑76 (MQ=255)                                 acacCTATACACTAAGTCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCa                                     <  1:1252280/76‑1 (MQ=255)                                 acacCTATACACTAAGTCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCa                                     <  1:1252298/76‑1 (MQ=255)                                   acCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCAcc                                   <  1:812342/76‑1 (MQ=255)                                   acCTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCAcc                                   >  1:1336595/1‑76 (MQ=255)                                     cTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCg                                 <  1:369998/76‑1 (MQ=255)                                     cTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCg                                 <  1:1742073/76‑1 (MQ=255)                                     cTATACACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCg                                 <  2:677164/76‑1 (MQ=255)                                       ataCACTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGcc                               >  1:536484/1‑76 (MQ=255)                                         acacTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAg                             >  2:526344/1‑76 (MQ=255)                                          cacTAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAgg                            <  2:901821/76‑1 (MQ=255)                                             tAAGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGgtgt                         <  1:218455/76‑1 (MQ=255)                                              aaGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGtgtg                        >  1:453393/1‑76 (MQ=255)                                              aaGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGtgtg                        <  2:265186/76‑1 (MQ=255)                                               aGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGa                       <  1:315924/76‑1 (MQ=255)                                               aGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGa                       <  1:272078/76‑1 (MQ=255)                                               aGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGa                       >  2:1210936/1‑76 (MQ=255)                                               aGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGa                       >  2:808971/1‑76 (MQ=255)                                               aGGCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGa                       >  2:73906/1‑76 (MQ=255)                                                 gCTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACg                     >  1:531219/1‑76 (MQ=255)                                                  cTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAg                                        <  1:627069/56‑1 (MQ=255)                                                  cTATAAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAg                                        >  2:627069/1‑56 (MQ=255)                                                   tataAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGaa                   >  1:1170939/1‑76 (MQ=255)                                                    ataAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGaaa                  >  1:1296937/1‑76 (MQ=255)                                                    ataAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGaaa                  <  1:1697034/76‑1 (MQ=255)                                                     taAATGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGaaaa                 <  2:457726/76‑1 (MQ=255)                                                      aaaTGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGaaaaa                >  1:500023/1‑76 (MQ=255)                                                        aTGATATAGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAAc              >  1:583135/1‑76 (MQ=255)                                                               aGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTc       >  2:813613/1‑76 (MQ=255)                                                               aGTGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTc       >  1:29690/1‑76 (MQ=255)                                                                 tGGTTACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTg     >  2:897295/1‑76 (MQ=255)                                                                    ttACAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATg  <  2:262716/76‑1 (MQ=255)                                                                       |                                                                         GGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATG  >  REL606/1332062‑1332204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑