| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,388,714 | G→T | M331I (ATG→ATT) | gspF → | general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,388,714 | 0 | G | T | 98.3% | 224.9 / ‑3.6 | 60 | M331I (ATG→ATT) | gspF | general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (26/33); total (26/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
ACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTC > REL606/3388641‑3388786 | ccGTCCGCCAGGGTAACAGCATTCATTTTTCGCTGGAACAACCCGCAATTTTCCCGCCGATGATGCTCTACAttgt < 1:336770/75‑1 (MQ=255) ttCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAACCCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGc < 2:760401/76‑1 (MQ=255) ttCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGc > 2:1779400/1‑76 (MQ=255) tCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGcc > 1:319107/1‑76 (MQ=255) tCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGcc > 1:569753/1‑76 (MQ=255) cGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGCct > 1:129405/1‑76 (MQ=255) ccAGGGTAACAGCATTCATCTTTCGCTGGAACAACCCGCAATTTCCCCGCCGATGATGCTCTACATTGTGGCctct < 1:1654619/76‑1 (MQ=255) ccAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGCctct < 1:160261/76‑1 (MQ=255) ccAGGGAAACACCATTCATCTTCCGCTGGAACAAACCGCAATTTCCCCGCCGATGATGCTCTACATTGTGGCctct < 2:545107/76‑1 (MQ=255) ggTAACAGCATTCATCTTTCGCGGAAACAACCCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCg < 1:1559200/76‑1 (MQ=38) gTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGa > 1:1277618/1‑76 (MQ=255) gTAACAGCATTCATCTTTCGCTGGAACAAAACGCAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGa > 1:1472060/1‑76 (MQ=39) tAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGaa > 1:629241/1‑76 (MQ=255) aaCAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGaaa > 2:467036/1‑76 (MQ=255) aaCAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGaaa > 1:1729099/1‑76 (MQ=255) gCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAg > 1:1533798/1‑76 (MQ=255) gCATTCATCTTTCGCGGGAACAACCCGCACTTTCCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAg < 2:313961/76‑1 (MQ=38) cATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGc > 1:1104522/1‑76 (MQ=255) cATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGc > 1:1445247/1‑76 (MQ=255) tCATCTTTCGCTGGAACAAACCNCAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCggg > 2:257442/1‑76 (MQ=39) caccTTTCGCGAGAACAACCCGCAATTTCCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGc < 2:1605511/73‑1 (MQ=37) cATTTTTCGCTGGAACAACCCGAAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGc < 2:1151496/76‑1 (MQ=37) cATCTTTCGCTGGAACAACCCGCATTTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGc < 2:62962/76‑1 (MQ=38) ttCGCTGGAACAACCCGCAATTTCCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTc < 2:1830661/76‑1 (MQ=38) ttCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTc > 2:119356/1‑76 (MQ=255) ttCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTc > 2:174159/1‑76 (MQ=255) cGCTGAAACAACCCGCAATTTCCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCgg < 1:1504419/76‑1 (MQ=38) gAACAACCCGCAATTTCCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACAt < 1:170316/76‑1 (MQ=38) gAACAACCCGCAATTTCCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACAt < 1:1783173/76‑1 (MQ=38) aCAAACCTCAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTa < 2:276987/76‑1 (MQ=255) aCAAACCACAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTa < 2:1006773/76‑1 (MQ=255) aacccGCATTTTCCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAAt < 1:1561373/73‑1 (MQ=38) aacccGCAATTGTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAAt < 1:81895/73‑1 (MQ=38) gCAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCa > 1:719962/1‑76 (MQ=255) cAATTTTCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCag < 2:1328562/76‑1 (MQ=255) nttttCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCagag > 1:514899/2‑76 (MQ=255) aTTTCCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCagag < 2:608844/76‑1 (MQ=255) ttttCCCGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGc < 1:1835430/76‑1 (MQ=255) ttccccGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGcc < 1:84520/73‑1 (MQ=255) cccGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCa < 2:1236047/76‑1 (MQ=255) cccGCCGATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCa > 2:42069/1‑76 (MQ=255) ccgccgATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAg < 1:1313660/76‑1 (MQ=255) cgccgATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGa > 1:1554703/1‑76 (MQ=255) gccgATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGAt < 2:1682873/76‑1 (MQ=255) ccgATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATa > 1:32253/1‑76 (MQ=255) ccgATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATa < 2:200403/76‑1 (MQ=255) cgATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATaa < 2:73583/76‑1 (MQ=255) cgATGATGCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATaa < 2:1332004/76‑1 (MQ=255) atgatgCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAAcc < 1:435003/76‑1 (MQ=255) tgatgCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCa > 2:761098/1‑76 (MQ=255) tgatgCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCa < 1:1666896/76‑1 (MQ=255) gatgCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAg < 1:121647/76‑1 (MQ=255) gatgCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAg < 1:1133978/76‑1 (MQ=255) atgCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAgg > 2:1071783/1‑76 (MQ=255) atgCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAgg < 1:1128382/76‑1 (MQ=255) tgCTCTACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGga > 1:1398210/1‑76 (MQ=255) ctACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGaca > 2:1113670/1‑76 (MQ=255) tACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGacac > 2:1333818/1‑76 (MQ=255) tACATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGacac < 1:1620874/76‑1 (MQ=255) aCATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACt < 1:431100/76‑1 (MQ=255) cATTGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTc > 1:646078/1‑76 (MQ=255) | ACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTC > REL606/3388641‑3388786 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |