BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,465,186	C→T	G28D (GGC→GAC)	ompR ←	osmolarity response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,465,186	0	C	T	100.0%	220.6 / NA	56	G28D (GGC→GAC)	ompR	osmolarity response regulator
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (33/23); total (33/23)

ATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGT  >  REL606/3465114‑3465258

aTAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCtt                                                                       <  1:923534/76‑1 (MQ=255)

 tAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTg                                                                      >  2:1607613/1‑76 (MQ=255)

  aaGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGt                                                                     >  2:89426/1‑76 (MQ=255)

    gATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTc                                                                   <  2:1155258/76‑1 (MQ=255)

      tGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCgg                                                                 >  2:1588369/1‑76 (MQ=255)

       ggAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGt                                                                >  2:987681/1‑76 (MQ=255)

       ggAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGt                                                                >  2:464773/1‑76 (MQ=255)

       ggAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGt                                                                >  1:283078/1‑76 (MQ=255)

         aaaGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTga                                                              >  2:1001058/1‑76 (MQ=255)

             aTTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATa                                                          >  2:182306/1‑76 (MQ=255)

              ttCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATaa                                                         <  1:940319/76‑1 (MQ=255)

              ttCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATaa                                                         >  1:702356/1‑76 (MQ=255)

                 aCGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGGGATAACGt                                                      >  2:908023/1‑76 (MQ=255)

                   gAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTc                                                    <  2:1670247/76‑1 (MQ=255)

                       cagcagGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCcagc                                                >  2:883045/1‑76 (MQ=255)

                          cagGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCagcagc                                             >  2:1512516/1‑76 (MQ=255)

                           agGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAgcg                                            >  2:980816/1‑76 (MQ=255)

                           agGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAgcg                                            <  1:376571/76‑1 (MQ=255)

                           agGCGATCCATCTGTTCTGCATTAGCGACACTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAgcg                                            >  1:170546/1‑76 (MQ=255)

                             gCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGcgca                                          >  1:88220/1‑76 (MQ=255)

                              cGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGcgcac                                         >  1:450359/1‑76 (MQ=255)

                               gATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGcgcacg                                        >  2:892835/1‑76 (MQ=255)

                                    aTCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGgc                                   >  1:833149/1‑76 (MQ=255)

                                    aTCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGgc                                   >  1:641638/1‑76 (MQ=255)

                                     tCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGgcg                                  <  2:1739394/76‑1 (MQ=255)

                                     tCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGgcg                                  <  2:16701/76‑1 (MQ=255)

                                     tCTGTTCTGCATTAGCAACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGgcg                                  >  2:803641/1‑76 (MQ=255)

                                       tGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCa                                >  1:1452029/1‑76 (MQ=255)

                                          tCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATgt                             >  1:978222/1‑76 (MQ=255)

                                            tGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATgtcg                           <  1:187741/76‑1 (MQ=255)

                                            tGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATgtcg                           <  1:1454268/76‑1 (MQ=255)

                                             gCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATgtcgt                          >  2:458959/1‑76 (MQ=255)

                                              cATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATgtcgtc                         >  2:1732860/1‑76 (MQ=255)

                                               aTTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCa                        <  1:480029/76‑1 (MQ=255)

                                                 tAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATc                      <  2:450359/76‑1 (MQ=255)

                                                  aGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCg                     <  1:1800490/76‑1 (MQ=255)

                                                  aGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACATTCCAGCAGCGCACGCAGGCGCATGTCGTCATCg                     >  2:1351598/1‑76 (MQ=255)

                                                     gACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGacc                  <  1:1607613/76‑1 (MQ=255)

                                                       cGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGaccac                <  2:1826554/76‑1 (MQ=255)

                                                        gCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGaccacc               >  1:6900/1‑76 (MQ=255)

                                                         cTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGAccacca              >  2:1799088/1‑76 (MQ=255)

                                                          ttCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAg             <  1:1063795/76‑1 (MQ=255)

                                                           tCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGa            >  2:630335/1‑76 (MQ=255)

                                                            cGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGaa           >  2:45544/1‑76 (MQ=255)

                                                              aaCCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAata         <  2:457939/76‑1 (MQ=255)

                                                              aaCCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAata         <  2:1697578/76‑1 (MQ=255)

                                                               aCCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAatat        <  1:823103/76‑1 (MQ=255)

                                                               aCCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAatat        <  2:843668/76‑1 (MQ=255)

                                                                ccTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATAtt       <  2:142636/76‑1 (MQ=255)

                                                                ccTGGAAGTCTTGTTCGGTGAGATAACGTACCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATAtt       <  2:316634/76‑1 (MQ=255)

                                                                 cTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTg      >  1:1809504/1‑76 (MQ=255)

                                                                  tGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGt     <  2:847062/76‑1 (MQ=255)

                                                                  tGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGt     >  2:115917/1‑76 (MQ=255)

                                                                    gAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAg   >  2:749456/1‑76 (MQ=255)

                                                                    gAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAg   <  1:1823644/76‑1 (MQ=255)

                                                                     aaGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGt  >  1:96159/1‑76 (MQ=255)

ATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGT  >  REL606/3465114‑3465258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`