| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,162,570 | T→A | I572N (ATC→AAC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,162,570 | 0 | T | A | 95.7% | 159.3 / 0.7 | 46 | I572N (ATC→AAC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base A (22/22); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01 | |||||||||||
GAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAC > REL606/4162498‑4162639 | gagaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGATNCCCATGAAGCTCCGAACATCGGTCTGAACaa < 1:107969/76‑1 (MQ=255) cGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAActct < 2:855588/76‑1 (MQ=255) tACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAActctct < 1:165836/76‑1 (MQ=255) tACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAActctct > 2:66925/1‑76 (MQ=255) acacCCGACTCACTACGNTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCNCACATCTGTATAAACAACTCTCTg > 2:950246/1‑76 (MQ=255) cccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTGTGAACAACTCTCTGTcc > 2:166970/1‑76 (MQ=255) aCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCgtgt < 1:165144/76‑1 (MQ=255) cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTa > 2:245712/1‑76 (MQ=255) tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTAc > 2:281412/1‑76 (MQ=255) tACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGcac < 1:737448/76‑1 (MQ=255) cGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAg < 1:8972/76‑1 (MQ=255) tCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACt > 2:267088/1‑76 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTa > 1:625077/1‑76 (MQ=255) gcgTATGTCCAATCGAAACCCCTGAAGGTCCNGACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTaa > 1:104148/1‑76 (MQ=255) gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCAAAGACTAACg > 2:240286/1‑76 (MQ=255) tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGa > 1:278686/1‑76 (MQ=255) tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGa < 1:245712/76‑1 (MQ=255) tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAAt < 2:551498/76‑1 (MQ=255) tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAAt < 1:195147/76‑1 (MQ=255) gTCCAATCGAAACCCCTGAAGGTCNGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATa > 2:204462/1‑76 (MQ=255) gTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATa < 1:950246/76‑1 (MQ=255) tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc < 2:131865/76‑1 (MQ=255) tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAAATCTCTGTCCGTGTACGCACAGACTAACGAATAc > 1:945456/1‑76 (MQ=255) cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg < 2:511562/76‑1 (MQ=255) cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg > 1:37801/1‑76 (MQ=255) cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg > 1:582568/1‑76 (MQ=255) aaTCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc > 1:207316/1‑76 (MQ=255) aaTCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc < 2:242979/76‑1 (MQ=255) tggaGGTCCGAACATCGGTCTGAACANCTCTCTATCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGATACt > 2:993001/4‑76 (MQ=255) gAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTc < 1:35097/76‑1 (MQ=255) gAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTCGAGACTc < 1:720000/76‑1 (MQ=255) gAAGGTCCGAACAGCGGTCTGAACAACTCTCTGTCCGTGTACTCACAGACTAACGAATACGGCTTCCTTGAGACTc > 1:743180/1‑76 (MQ=255) aaGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc > 1:937327/1‑76 (MQ=255) aGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCg < 2:1088977/76‑1 (MQ=255) aGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCg > 1:214030/1‑76 (MQ=255) ggTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt < 2:328691/76‑1 (MQ=255) tCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTAt < 2:830720/76‑1 (MQ=255) ccGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATc > 1:366774/1‑76 (MQ=255) cGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCg < 2:1095278/76‑1 (MQ=255) cGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCg < 1:215108/76‑1 (MQ=255) cGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGCCTTCCTTGAGACTCCGTATCg > 2:369594/1‑76 (MQ=255) aaCATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa > 2:357103/1‑76 (MQ=255) aaCATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa < 1:27069/76‑1 (MQ=255) aCATCGGCCTGAACAACTCTCTGTCCGCGTACGCACATACTCAAGCATACGGCTTCCTTGTTACTCCGTATCGTaa > 1:1037054/1‑76 (MQ=255) cGGTCTGAACAACTCTCTGTCCGTGTACGCACACACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTg < 1:920608/76‑1 (MQ=255) ttCTGAACAACGCTCTGATCGGTTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAc < 1:118704/75‑1 (MQ=255) gTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTTAGACTCCGTATCGTAAAGGGAc > 2:783758/1‑76 (MQ=255) gTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAc > 1:686194/1‑76 (MQ=255) | GAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAC > REL606/4162498‑4162639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |