| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 647,808 | T→G | K364Q (AAA→CAA) | mrdB ← | cell wall shape‑determining protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 647,808 | 0 | T | G | 100.0% | 74.2 / NA | 19 | K364Q (AAA→CAA) | mrdB | cell wall shape‑determining protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (9/10); total (9/10) | |||||||||||
CTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAAC > REL606/647738‑647879 | cTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTGCCtgt > 1:83113/1‑76 (MQ=255) aGATCCCGAGCCACTGCTTACGTATTGCGCACCTCTTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTg < 1:831568/76‑1 (MQ=255) aGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTg < 1:714544/76‑1 (MQ=255) ccGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGAGTGACAtt < 1:759544/76‑1 (MQ=255) cGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTGACATTa > 1:740761/1‑76 (MQ=255) aGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTGACATTACa < 2:645655/76‑1 (MQ=255) ccACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTGACATTACAAt > 2:805462/1‑76 (MQ=255) cACTGCTTACGTATTTCTCACCTCATACACAATTTGCGACAANANTTGCCAGNGGGTGTGGATTGACATTACAATc < 1:1068406/76‑1 (MQ=255) tGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTGACATTACAATCCCg < 1:1078840/76‑1 (MQ=255) cTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTGACATTACAATCCCGaa > 2:102279/1‑76 (MQ=255) aCGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTGACATTACAATCCCGAAccc < 2:362511/76‑1 (MQ=255) cGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCa < 2:617565/76‑1 (MQ=255) tGCGCACCTCTTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCAt < 1:700837/76‑1 (MQ=255) gcACCTCTTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAg < 2:251426/76‑1 (MQ=255) gcACCTCTTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAg > 2:397862/1‑76 (MQ=255) ctTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAAt > 2:1137984/1‑76 (MQ=255) ctTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAAt > 2:659350/1‑76 (MQ=255) ctTACACGCTTTTCGACAACATTTGCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAAt < 1:248947/76‑1 (MQ=255) gCTTTTCGACAACATTTGCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGcc > 2:490657/1‑76 (MQ=255) aTTTGCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAAc > 2:755454/1‑76 (MQ=255) | CTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAAC > REL606/647738‑647879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |