| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,875,625 | 1 | . | T | 95.8% | 186.2 / 0.5 | 48 | intergenic (‑59/+294) | glmU/atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base T (25/21); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
TAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑TT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCG > REL606/3875551‑3875693 | tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑t < 1:912336/76‑1 (MQ=255) aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑tt > 1:1527059/1‑76 (MQ=255) ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCANCTTGAAAAAACTAC‑A‑tt‑tttt > 2:301361/1‑76 (MQ=255) ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑tt‑tttt > 1:1140858/1‑76 (MQ=255) ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑tt‑tttt > 1:264020/1‑76 (MQ=255) ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑tt‑tttt > 1:1683538/1‑76 (MQ=255) ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑tt‑tttt < 2:1067751/76‑1 (MQ=255) attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑tt‑tttttt > 2:1635461/1‑75 (MQ=255) ttCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑tt‑ttttttc < 1:216145/76‑3 (MQ=255) aCATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCg < 1:1501286/76‑1 (MQ=255) cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGt < 1:1256/76‑1 (MQ=255) aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTg < 1:136985/76‑1 (MQ=255) aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTg < 2:225955/76‑1 (MQ=255) aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTg < 1:1200584/76‑1 (MQ=255) tACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGa > 2:668130/1‑76 (MQ=255) gcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAAt < 2:831782/76‑1 (MQ=255) cgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATg > 2:181379/1‑76 (MQ=255) cgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATg > 1:499519/1‑76 (MQ=255) tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACag < 1:396072/76‑1 (MQ=255) tGACTGTAATTTGAGAACAAATTAAACCCGCTTCACCTTAAAAAAACTACTT‑TT‑TTTTTCATCGTGAAATGGACag < 2:1029276/76‑1 (MQ=255) aCTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACagag > 1:1688325/1‑76 (MQ=255) aCTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACagag < 1:175268/76‑1 (MQ=255) gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGAt < 2:906453/76‑1 (MQ=255) tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATa < 1:1316789/76‑1 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATaaa > 1:1246924/1‑76 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAAtt > 1:398092/1‑76 (MQ=255) gagaACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGt > 2:277553/1‑76 (MQ=255) aCGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCaa > 2:745392/1‑76 (MQ=255) cGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt > 1:1224445/1‑76 (MQ=255) gAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa < 1:28429/76‑1 (MQ=255) gAATTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa > 1:1442145/1‑76 (MQ=255) aaTTTAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa < 1:671404/76‑1 (MQ=255) ttAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg > 2:1845748/1‑76 (MQ=255) ttAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg > 2:1764261/1‑76 (MQ=255) ttAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg < 2:1558319/76‑1 (MQ=255) tAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 2:1110642/1‑76 (MQ=255) tAAACCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 2:696097/1‑76 (MQ=255) aaaCCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt < 1:840132/76‑1 (MQ=255) aCCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa > 1:1540779/1‑76 (MQ=255) aCCGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGAGCAGAGGATAAATTGTTCAATAACGGATTa < 2:71936/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 1:525379/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 1:345027/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTAC‑A‑TTGTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 2:1306222/76‑1 (MQ=255) cGCTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATc > 1:731672/1‑76 (MQ=255) cTTCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATccc > 2:651941/1‑76 (MQ=255) tCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg > 2:1471886/1‑76 (MQ=255) tCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg < 2:704418/76‑1 (MQ=255) tCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg < 2:461654/76‑1 (MQ=255) tCACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg > 1:235035/1‑76 (MQ=255) cACCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGc > 1:1080495/1‑76 (MQ=255) aCCTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCt > 2:866139/1‑76 (MQ=255) ccTTGAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg > 1:832429/1‑76 (MQ=255) gAAAAAACTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGa > 1:238012/1‑76 (MQ=255) aaaaaaCTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa < 1:1281745/76‑1 (MQ=255) aaaaaaCTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa < 2:220373/76‑1 (MQ=255) aaaaCTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg > 2:880286/1‑76 (MQ=255) aaCTAC‑ATTT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcg > 1:1190979/1‑76 (MQ=255) | TAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTAC‑A‑TT‑TTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCG > REL606/3875551‑3875693 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |