Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,098,752	G→A	V75V (GTG→GTA)	ycdO →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,098,752	0	G	A	100.0%	159.7 / NA	43	V75V (GTG→GTA)	ycdO	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/24);  total (19/24)

AAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATA  >  REL606/1098681‑1098823                                                                        |                                                                        aaaaCACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGAtggtagtgg                                                                     >  2:936374/1‑76 (MQ=255) aaaaCACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGAtggtagtgg                                                                     >  2:109823/1‑76 (MQ=255)   aaCACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGaa                                                                   >  2:451502/1‑76 (MQ=255)    acacAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAag                                                                  <  2:986472/76‑1 (MQ=255)    acacAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAag                                                                  <  2:1456548/76‑1 (MQ=255)     cacaGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAaga                                                                 <  1:416679/76‑1 (MQ=255)      acaGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAagag                                                                <  2:1712512/76‑1 (MQ=255)        aGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCg                                                              <  1:706301/76‑1 (MQ=255)          ttCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCggg                                                            <  1:839328/76‑1 (MQ=255)           tCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGa                                                           <  2:1418887/76‑1 (MQ=255)             attattCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGaaa                                                         >  2:348050/1‑76 (MQ=255)             attattCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGaaa                                                         <  1:1825241/76‑1 (MQ=255)               tattCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAat                                                       <  1:1803779/76‑1 (MQ=255)                attCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAata                                                      >  1:1020714/1‑76 (MQ=255)                attCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAata                                                      >  1:562408/1‑76 (MQ=255)                 ttCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAatat                                                     >  1:1181138/1‑76 (MQ=255)                   cAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCg                                                   <  1:1850309/76‑1 (MQ=255)                    aGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGc                                                  <  1:719619/76‑1 (MQ=255)                        ccACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCt                                              <  1:664109/76‑1 (MQ=255)                        ccACAGCCAGAAGGCGCGGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCt                                              <  1:1693579/76‑1 (MQ=255)                         cacaGCCAGAAGGGGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGGGGAAGAGCGGGAAAATATCGCCCCTg                                             <  2:129705/76‑1 (MQ=255)                           caGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGc                                           >  2:1351632/1‑76 (MQ=255)                             gCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCtt                                         <  1:1256124/76‑1 (MQ=255)                             gCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCtt                                         <  1:1447374/76‑1 (MQ=255)                               cAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTa                                       >  2:125149/1‑76 (MQ=255)                                aGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAg                                      <  1:583394/76‑1 (MQ=255)                                 gAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGc                                     >  1:527734/1‑76 (MQ=255)                                   aGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCa                                   <  2:423281/76‑1 (MQ=255)                                   aGGCGCTAGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCa                                   <  1:50100/76‑1 (MQ=255)                                    ggCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAg                                  <  1:141378/76‑1 (MQ=255)                                          ggAGTGGGAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATg                            >  2:1773937/1‑76 (MQ=255)                                               gggAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGc                       >  2:1314175/1‑76 (MQ=255)                                               gggAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGc                       >  2:1336912/1‑76 (MQ=255)                                                ggAGATCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCg                      >  1:384808/1‑76 (MQ=255)                                                  agaTCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGAAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGaa                    >  2:599716/1‑76 (MQ=255)                                                    aTCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAAtt                  >  2:127047/1‑76 (MQ=255)                                                    aTCCTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAAtt                  >  1:1730631/1‑76 (MQ=255)                                                      ccTAAAGGGCGTGATGGTGGGGGAAGAGGGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTa                <  1:629525/76‑1 (MQ=255)                                                       cTCAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTAc               <  2:1277100/76‑1 (MQ=255)                                                         cAAAGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAg             >  1:190746/1‑76 (MQ=255)                                                          aaaGGCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGc            >  2:411273/1‑76 (MQ=255)                                                            aGGGGTGATGGTAGGGGAAGAGGGGAAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCt          <  1:895836/76‑1 (MQ=255)                                                             ggCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTg         <  2:898904/76‑1 (MQ=255)                                                              gCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTgg        <  2:1442725/76‑1 (MQ=255)                                                              gCGTGATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTgg        <  2:1164495/76‑1 (MQ=255)                                                                gTGAGGGTAGGGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCg      <  1:1542174/76‑1 (MQ=255)                                                                  gATGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAACCAGAAAATGACGGCGAATTTACAGCCTGGCGaa    >  1:1621436/1‑76 (MQ=255)                                                                   aTGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAAt   <  2:403132/76‑1 (MQ=255)                                                                   aTGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAAt   <  2:754338/76‑1 (MQ=255)                                                                   aTGGTAGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAAt   >  2:1504350/1‑76 (MQ=255)                                                                    tggtagtggAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATa  >  2:1179754/1‑76 (MQ=255)                                                                    tggtagtggAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATa  <  1:74056/76‑1 (MQ=255)                                                                        |                                                                        AAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATA  >  REL606/1098681‑1098823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑