Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,329,516	0	C	T	100.0%	155.1 / NA	41	H33Y (CAC→TAC)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/21);  total (20/21)

TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329443‑1329588                                                                          |                                                                          tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac                                                                         <  1:242163/76‑1 (MQ=255)   aGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACAt                                                                       <  1:1140179/76‑1 (MQ=255)   aGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACAt                                                                       <  2:1299130/76‑1 (MQ=255)   aGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACAt                                                                       <  1:174236/76‑1 (MQ=255)    gTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATc                                                                      <  2:717981/76‑1 (MQ=255)     tCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATcc                                                                     <  2:688271/76‑1 (MQ=255)      ccccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcg                                                                    <  2:1032028/76‑1 (MQ=255)        ccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGAGGTGAAATCCAGCGTCGGTTACATCcgcg                                                                  >  2:312866/1‑76 (MQ=255)            cAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCNGCGTCGGTTACATCCGCGAttt                                                              <  2:257797/76‑1 (MQ=255)             aaaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTg                                                             >  2:934675/1‑76 (MQ=255)             aaaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTg                                                             >  2:1042265/1‑76 (MQ=255)                   cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                       >  1:1654652/1‑76 (MQ=255)                    aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCa                                                      >  1:1491195/1‑76 (MQ=255)                     aaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAg                                                     >  2:586376/1‑76 (MQ=255)                      aaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGt                                                    <  2:25947/76‑1 (MQ=255)                       aCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTg                                                   <  2:37478/76‑1 (MQ=255)                        cGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTgg                                                  >  1:582851/1‑76 (MQ=255)                           tCAACAAGTATCTGGGTAGTGACTACGTGGTNAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTc                                               <  1:1535569/76‑1 (MQ=255)                           tCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTc                                               <  2:179747/76‑1 (MQ=255)                           tCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTc                                               >  1:1423332/1‑76 (MQ=255)                            caacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCa                                              <  2:858679/76‑1 (MQ=255)                             aacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAg                                             >  2:412641/1‑76 (MQ=255)                               caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                           >  2:1768797/1‑76 (MQ=255)                                 aGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGc                                         >  2:1481842/1‑76 (MQ=255)                                  gTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGcc                                        <  1:103160/76‑1 (MQ=255)                                   tATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑a                                      >  2:1133806/1‑76 (MQ=255)                                    aTCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑aa                                     <  1:1019137/76‑1 (MQ=255)                                      cTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑aaaa                                   >  1:1307888/1‑76 (MQ=255)                                           tAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCCCAGCTGCC‑AAAAagagc                              >  1:1416716/1‑75 (MQ=255)                                            aGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTg                             >  2:1318402/1‑76 (MQ=255)                                                aCTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCCCAGCTGCC‑AAAAAAAGTGCCGa                         >  1:806766/1‑76 (MQ=255)                                                    cGTGGTGAAATCCGGCGTCGTTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGActct                     <  1:1365596/76‑1 (MQ=255)                                                     gtggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTa                    <  2:208850/76‑1 (MQ=255)                                                     gtggtgAAACCCAGCGTCGTTTACACCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTa                    <  1:1133806/76‑1 (MQ=255)                                                     agggtaAAGTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTa                    <  2:1605039/74‑1 (MQ=255)                                                       ggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTAcc                  <  1:628072/76‑1 (MQ=255)                                                         tgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTACCTc                <  2:1483614/76‑1 (MQ=255)                                                            aaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTACCTccac             <  2:1307888/76‑1 (MQ=255)                                                            aaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTACCTccac             >  1:669651/1‑76 (MQ=255)                                                              tCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAAGAGTGCCGACTCTACCTccacc            <  1:1308120/76‑1 (MQ=255)                                                              tCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTACCTccacca           <  1:1576736/76‑1 (MQ=255)                                                                cggCGTCGGTTACATCCGCGATTTGCCGCCCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAg         <  1:496608/74‑1 (MQ=255)                                                                cAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAg         >  1:969878/1‑76 (MQ=255)                                                                      cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt   >  1:52745/1‑76 (MQ=255)                                                                       ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  <  1:509777/76‑1 (MQ=255)                                                                       ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  >  2:917286/1‑76 (MQ=255)                                                                       ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  <  2:1596011/76‑1 (MQ=255)                                                                          |                                                                          TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCC‑AAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329443‑1329588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑