Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,876,460	A→C	L115* (TTA→TGA)	araE ←	arabinose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,876,460	0	A	C	100.0%	212.9 / NA	51	L115* (TTA→TGA)	araE	arabinose transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/21);  total (30/21)

TTCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAG  >  REL606/2876387‑2876530                                                                          |                                                                       ttCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCAc                                                                      <  2:1696828/76‑1 (MQ=255) ttCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCAc                                                                      <  1:20840/76‑1 (MQ=255) ttCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCAc                                                                      >  2:1719972/1‑76 (MQ=255)    aGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAAGGCCCAGCACCACACGAGCGGCGACTCACAtc                                                                   >  1:1124168/1‑76 (MQ=255)      aaaGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACAtctc                                                                 >  1:1722677/1‑76 (MQ=255)      aaaGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACAtctc                                                                 >  2:731430/1‑76 (MQ=255)       aaGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATctct                                                                >  2:1464497/1‑76 (MQ=255)        aGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTa                                                               >  2:925999/1‑76 (MQ=255)         gATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTAc                                                              <  2:1846873/76‑1 (MQ=255)          aTACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACg                                                             <  2:776924/76‑1 (MQ=255)           tACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGc                                                            <  1:800751/76‑1 (MQ=255)            aCAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGcc                                                           >  2:898896/1‑75 (MQ=255)            aCAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCt                                                           >  1:502965/1‑76 (MQ=255)            aCAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCt                                                           >  2:576688/1‑76 (MQ=255)            aCAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCt                                                           >  1:308769/1‑76 (MQ=255)            aCAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCt                                                           >  2:898899/1‑76 (MQ=255)               gaggagCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGt                                                        >  2:1770234/1‑76 (MQ=255)               gaggagCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGt                                                        >  2:1316916/1‑76 (MQ=255)                aggagCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTc                                                       >  2:451503/1‑76 (MQ=255)                aggagCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTc                                                       >  2:1405229/1‑76 (MQ=255)                  gagCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGc                                                     <  1:281390/76‑1 (MQ=255)                     cGGTGTAAGGCGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCaaa                                                  <  1:1456607/76‑1 (MQ=255)                     cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCaaa                                                  <  2:1263644/76‑1 (MQ=255)                      ggTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCaaaa                                                 >  1:869546/1‑76 (MQ=255)                       gtgtAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAg                                                <  2:617502/76‑1 (MQ=255)                          tAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCgg                                             >  1:218818/1‑76 (MQ=255)                               cgcgATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCt                                        >  2:1847007/1‑76 (MQ=255)                                 cgATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCtat                                      <  1:1154711/76‑1 (MQ=255)                                 cgATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCtat                                      >  2:1532765/1‑76 (MQ=255)                                 cgATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCtat                                      <  1:749029/76‑1 (MQ=255)                                  gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCtata                                     >  2:1476870/1‑76 (MQ=255)                                   aTCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAg                                    >  2:160833/1‑76 (MQ=255)                                   aTCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAg                                    >  2:1641980/1‑76 (MQ=255)                                   aTCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAAACTCTCTACGCTGGTCGAAAAAGCGGCCCCtataa                                    >  2:1129181/1‑75 (MQ=255)                                    tCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGa                                   <  1:1442687/76‑1 (MQ=255)                                      ccGACAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAAc                                 <  2:838459/76‑1 (MQ=255)                                          cAGCAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAg                             >  2:1727357/1‑76 (MQ=255)                                            gCAATGCCCAGCCCCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTa                           <  1:618109/76‑1 (MQ=255)                                             cAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTAc                          <  1:478071/76‑1 (MQ=255)                                             cAATGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTAc                          <  1:925679/76‑1 (MQ=255)                                              aaTGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACa                         <  1:234682/76‑1 (MQ=255)                                              aaTGCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACa                         >  2:1813817/1‑76 (MQ=255)                                                 gCCCAGCACCACACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAAc                      <  1:1861732/76‑1 (MQ=255)                                                       caccacACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATg                >  1:790248/1‑76 (MQ=255)                                                         ccacACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGc              <  2:224367/76‑1 (MQ=255)                                                         ccacACGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGc              >  2:1010401/1‑76 (MQ=255)                                                          cacaCGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGcc             >  2:1864756/1‑76 (MQ=255)                                                           acacGAGCGGCGATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGccc            >  2:1213453/1‑76 (MQ=255)                                                                  cggcgTTTCACTTCTCTACGCTGGACGCAAAAGCGGACCCTATAGAGCCGAGTACAAACAGAATGGCCCCCGCCAt     <  2:971842/76‑1 (MQ=255)                                                                   ggcgATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCCTc    >  1:75363/1‑76 (MQ=255)                                                                    gcgATTCACATCTCTCCGTTGTTCGCAAAGGCGGTCCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCa   <  1:1267971/76‑1 (MQ=255)                                                                    gcgATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCa   <  1:1477739/76‑1 (MQ=255)                                                                    gcgATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCa   <  2:1243584/76‑1 (MQ=255)                                                                     cgATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAg  >  2:1076083/1‑76 (MQ=255)                                                                     cgATTCACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAg  >  2:1597841/1‑76 (MQ=255)                                                                          |                                                                       TTCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCGCCATCAG  >  REL606/2876387‑2876530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑