Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,252,006	A→G	V252A (GTA→GCA)	nusA ←	transcription elongation factor NusA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,252,006	0	A	G	100.0%	157.8 / NA	40	V252A (GTA→GCA)	nusA	transcription elongation factor NusA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/20);  total (20/20)

ACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCG  >  REL606/3251935‑3252077                                                                        |                                                                        acaGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCa                                                                     <  2:1300415/76‑1 (MQ=255)  caGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCaa                                                                    >  2:368288/1‑76 (MQ=255)        gATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCt                                                              <  2:1342045/76‑1 (MQ=255)             cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCgg                                                         >  2:687651/1‑76 (MQ=255)             cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCgg                                                         >  2:66330/1‑76 (MQ=255)             cGATACGCTCGCCACCCAGTTCAGCAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCgg                                                         <  1:1133276/76‑1 (MQ=255)              gATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGa                                                        >  1:4312/1‑76 (MQ=255)                 aCGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCg                                                     <  2:494739/76‑1 (MQ=255)                  cGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGa                                                    >  2:1166711/1‑76 (MQ=255)                  cGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGa                                                    >  2:1831184/1‑76 (MQ=255)                   gCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGAt                                                   <  1:1825114/76‑1 (MQ=255)                      cGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCACCACGCATACCAGCGCNAGCACCTACCGGATCGATACg                                                >  2:294473/1‑76 (MQ=255)                       gCCACCCAGTTCAGTAGACACCGCCTGTACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGt                                               >  2:1837494/1‑76 (MQ=255)                         cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGttt                                             >  2:1057392/1‑76 (MQ=255)                         cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGttt                                             <  1:1123419/76‑1 (MQ=255)                           cccAGTTCAGTAGACCCCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTAt                                           <  2:1655354/76‑1 (MQ=255)                             cAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCg                                         <  2:732984/76‑1 (MQ=255)                                 tCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTgg                                     <  1:223009/76‑1 (MQ=255)                                 tCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTgg                                     <  1:485830/76‑1 (MQ=255)                                 tCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTgg                                     >  1:1159334/1‑76 (MQ=255)                                     tAGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGANCGATACGTTTATCGTTGGtttt                                 <  2:256471/76‑1 (MQ=255)                                     tAGACACCGCCGGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGtttt                                 <  2:1278010/76‑1 (MQ=255)                                      aGACACCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTc                                <  2:853031/76‑1 (MQ=255)                                          acCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACcg                            <  1:308396/76‑1 (MQ=255)                                          acCGCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACcg                            >  1:1531336/1‑76 (MQ=255)                                          acCGCCTGAACACGCGCGCAACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACcg                            >  1:1659660/1‑76 (MQ=255)                                             gCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGa                         >  2:1463257/1‑76 (MQ=255)                                             gCCTGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGa                         >  1:151145/1‑76 (MQ=255)                                                tGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAttt                      >  1:1724977/1‑76 (MQ=255)                                                tGAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAttt                      >  2:1142037/1‑76 (MQ=255)                                                 gAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGCTACGTTTATCGTTGGTTTTCACCGCGAtttt                     >  2:1346277/1‑76 (MQ=255)                                                 gAACACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAtttt                     >  2:1659202/1‑76 (MQ=255)                                                  aaCACGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTc                    <  2:791754/76‑1 (MQ=255)                                                     acGCGCGCCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCa                 >  1:1236578/1‑76 (MQ=255)                                                       gcgcgcCGCGCATGCCTGCGCAAGCACCTGCCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACg               <  2:163172/76‑1 (MQ=255)                                                       gcgcgcCACGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACg               <  2:1026898/76‑1 (MQ=255)                                                               cGCATACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCg       <  1:515753/76‑1 (MQ=255)                                                                  aTACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt    <  2:1132608/76‑1 (MQ=255)                                                                  aTACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt    <  1:665610/76‑1 (MQ=255)                                                                   tACCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAta   >  2:1047268/1‑75 (MQ=255)                                                                    aCCTGCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATcg  <  2:1756002/76‑1 (MQ=255)                                                                        |                                                                        ACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCG  >  REL606/3251935‑3252077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑