| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,616,302 | (G)7→6 | intergenic (‑42/‑354) | purL ← / → yfhD | phosphoribosylformylglycinamidine synthase/putative periplasmic binding protein of transport system; b2558_1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,616,296 | 0 | G | . | 94.7% | 259.7 / 8.5 | 57 | intergenic (‑36/‑360) | purL/yfhD | phosphoribosylformylglycinamidine synthase/putative periplasmic binding protein of transport system; b2558_1 |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base . (31/23); total (32/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
ATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACC > REL606/2616221‑2616367 | aTGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAg < 2:939039/76‑1 (MQ=255)aTGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAg > 1:1367557/1‑76 (MQ=255)aTGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAg < 1:932048/76‑1 (MQ=255) tcccGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAgg < 1:727272/74‑1 (MQ=255) tGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAgg > 1:955807/1‑76 (MQ=255) tGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAgg > 2:357562/1‑76 (MQ=255) gCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAggg < 1:438024/76‑1 (MQ=255) gACCGTGCCGGCGAACCCCGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAgggggg < 1:751214/76‑1 (MQ=255) aGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGaaa < 1:1528478/76‑1 (MQ=255) gTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGaaac > 1:1627434/1‑75 (MQ=255) gTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGaaac < 1:240499/76‑2 (MQ=255) tGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTTCCCAA‑GGGGGGAAACg < 2:1147057/76‑1 (MQ=255) tGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACg > 1:1166773/1‑76 (MQ=255) tGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACg > 1:755363/1‑76 (MQ=255) tGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACg < 2:1267318/76‑1 (MQ=255) gCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACgg < 1:1525080/76‑1 (MQ=255) cAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACggg > 2:599031/1‑76 (MQ=255) aGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGc < 2:145847/76‑1 (MQ=255) aGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGc > 2:587216/1‑76 (MQ=255) gCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGt > 2:337160/1‑76 (MQ=255) gCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGt < 1:213505/76‑1 (MQ=255) gAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTCCCCAA‑GGGGGGAAACGGGCGTCa < 2:682530/76‑1 (MQ=255) gAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCa < 2:981392/76‑1 (MQ=255) gAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCa < 2:994179/76‑1 (MQ=255) aaCCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCAt > 1:1241639/1‑76 (MQ=255) cACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATtat < 1:497144/76‑1 (MQ=255) cACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATtat < 1:990117/76‑1 (MQ=255) gCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATaaa < 1:1024749/76‑1 (MQ=255) gCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATaaa > 2:1776358/1‑76 (MQ=255) cAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAg < 2:1690769/76‑1 (MQ=255) aaTTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAAt > 1:667761/1‑76 (MQ=255) tttCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCt < 1:907931/76‑1 (MQ=255) ttCAATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTg > 2:1622661/1‑76 (MQ=255) atcatAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATgc > 1:826071/1‑76 (MQ=255) catAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCCTTATAAAGAATCTGATgcgc > 2:416324/1‑76 (MQ=255) atAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCt > 2:1430265/1‑76 (MQ=255) tAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTg > 2:1429051/1‑76 (MQ=255) ctctcGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGaa > 2:1087924/1‑76 (MQ=255) ctctcGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGaa < 2:1347823/76‑1 (MQ=255) ctctcGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGaa < 2:344122/76‑1 (MQ=255) ctcGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAAc > 1:198865/1‑76 (MQ=255) ctcGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAAc > 2:1812753/1‑76 (MQ=255) tcGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAAcc < 2:361240/76‑1 (MQ=255) cGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCg > 2:263612/1‑76 (MQ=255) cGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCg > 1:1821524/1‑76 (MQ=255) gTCTTCTTAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGt > 2:1138890/1‑76 (MQ=255) gTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGt > 2:1256329/1‑76 (MQ=255) tcttctAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGtt < 2:762550/76‑1 (MQ=255) tcttctAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGtt > 1:1444216/1‑76 (MQ=255) tctAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGc > 1:363225/1‑76 (MQ=255) ctAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCg > 1:934636/1‑76 (MQ=255) ctAAGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCg > 1:1214928/1‑76 (MQ=255) aaGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTg < 2:1719814/76‑1 (MQ=255) aaGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTg < 2:307974/76‑1 (MQ=255) aGCTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTgg < 2:355449/76‑1 (MQ=255) cTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGaa < 1:335422/76‑1 (MQ=255) cTTTCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGaa > 1:968863/1‑76 (MQ=255) tttCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGaaa > 1:900819/1‑76 (MQ=255) tttCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGaaa > 1:822967/1‑76 (MQ=255) ttCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAAt > 1:652521/1‑76 (MQ=255) ttCGGTGTACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAAt < 1:1187957/76‑1 (MQ=255) tgtACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAAt > 2:1297074/1‑76 (MQ=255) tgtACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAAt < 1:750788/76‑1 (MQ=255) tACCCAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCa > 2:471053/1‑76 (MQ=255) cccAA‑GGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCAcc > 1:312352/1‑76 (MQ=255) | ATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACC > REL606/2616221‑2616367 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |