| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,036,374 | (A)9→8 | coding (585/1113 nt) | wbbC ← | putative glycosyltransferase WbbC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,036,366 | 0 | A | . | 96.4% | 256.1 / 3.5 | 55 | coding (593/1113 nt) | wbbC | putative glycosyltransferase WbbC |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (29/24); total (30/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
CTAATTTTTTGAACTTTAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAGAAAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATATTGTAAGTTACAATAA > REL606/2036292‑2036441 | cTAATTTTTTGAACTTTAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAGaa > 2:796490/1‑76 (MQ=255) tAATTTTTTGAACTTTAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAGaaa < 1:1055720/76‑1 (MQ=255) tAATTTTTTGAACTTTAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAGaaa > 2:1414481/1‑76 (MQ=255) aaTTTTTTGAACTTTAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAGaaaa > 1:48185/1‑76 (MQ=255) aaTTTTTTGAACTTTAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAGaaaa > 1:1552793/1‑76 (MQ=255) ttttttGAACTTTAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAGaaaaan < 1:30568/76‑2 (MQ=255) ttttGAACTTTAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAGaaaaaaaa < 1:1167162/76‑1 (MQ=255) tttGAACTTTAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCACNGCACCCACCGAGaaaaaaaag > 2:289823/1‑75 (MQ=255) ttGAACTTTAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAGaaaaaaaagg > 1:1765982/1‑74 (MQ=255) tGAACTTTAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAGaaaaaaaaggt > 2:1078439/1‑73 (MQ=255) tGAACTTTAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAGaaaaaaaaggt > 2:1065994/1‑73 (MQ=255) aCTTTAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTca < 2:1061142/76‑1 (MQ=255) tttAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTcaca < 2:363743/76‑1 (MQ=255) tAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAAt > 2:50475/1‑76 (MQ=255) aGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATc < 1:1315938/76‑1 (MQ=255) aGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATc > 1:960708/1‑76 (MQ=255) aaaaGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCat > 2:591243/1‑76 (MQ=255) aaaaGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCat < 1:118327/76‑1 (MQ=255) aaaaGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCat > 2:885258/1‑76 (MQ=255) aaaGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCata > 1:1000464/1‑76 (MQ=255) tATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑CAAAAAAAGGTTCACAATCATATTGGTGta > 2:318006/1‑76 (MQ=255) tATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑CAAAAAAAGGTTCACAATCATATTGGTGta > 2:317997/1‑76 (MQ=255) tATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGta > 2:729847/1‑76 (MQ=255) atcatcAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGtat < 2:1681429/76‑1 (MQ=255) tcatcaAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGtata > 2:1277825/1‑76 (MQ=255) tcatcaAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGtata < 1:643449/76‑1 (MQ=255) tcatcaAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGtata < 1:643454/76‑1 (MQ=255) catcaAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATag < 1:474803/76‑1 (MQ=255) catcaAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATag < 2:220189/76‑1 (MQ=255) catcaAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATag < 2:1557318/76‑1 (MQ=255) atcaAATTGGTTTGGTTTTTTTCATTGAAAGGCCCCCCCGGAAAAAAAAAAGGTTCACAATCATATTGGTGTATag < 2:220173/76‑1 (MQ=255) tcaAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATagag < 2:1719230/76‑1 (MQ=255) caAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAgg > 1:482727/1‑76 (MQ=255) aTCGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGAtt < 2:863514/76‑1 (MQ=255) cGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCGCAATCATATTGGTGTATAGAGGAtttt > 1:1247036/1‑76 (MQ=255) cGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCGCAATCATATTGGTGTATAGAGGAtttt > 1:1247047/1‑76 (MQ=255) cGTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGAtttt < 2:389613/76‑1 (MQ=255) gTATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGAttttt < 2:1773854/76‑1 (MQ=255) tATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGAtttttt > 1:623861/1‑76 (MQ=255) tATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGAtttttt < 1:487538/76‑1 (MQ=255) tATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGAtttttt < 1:308050/76‑1 (MQ=255) tATTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGAtttttt > 1:1188595/1‑76 (MQ=255) aTTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTa > 1:670320/1‑76 (MQ=255) aTTGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTa > 1:249903/1‑76 (MQ=255) ttGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTaa < 2:28749/76‑1 (MQ=255) ttGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTaa > 2:436790/1‑76 (MQ=255) ttGTTTATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTaa > 2:1523864/1‑76 (MQ=255) ttATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATc > 2:412999/1‑76 (MQ=255) ttATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATc < 1:1432517/76‑1 (MQ=255) tATCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCa < 2:821325/76‑1 (MQ=255) aTCTTCATGCAAAGCACCCACCGAGAAAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCa > 2:1441883/1‑76 (MQ=255) tCTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCAcc > 1:1469931/1‑76 (MQ=255) cTTCATGCAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCt < 1:973958/76‑1 (MQ=255) cAAAGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGTTGTATAGAGGATTTTTTAAAATCACCTAatatat < 1:372661/76‑1 (MQ=255) aaaGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATAtt > 2:1378799/1‑76 (MQ=255) aaaGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATAtt > 2:912091/1‑76 (MQ=255) aaaGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATAtt < 1:1065098/76‑1 (MQ=255) aaGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATATTg > 2:947634/1‑76 (MQ=255) aaGCACCCACCGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATATTg < 2:147566/76‑1 (MQ=255) cacccaccGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATATTGTaa < 2:1056455/76‑1 (MQ=255) acccaccGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATATTGTAAg > 1:1745719/1‑76 (MQ=255) caccGAGAAAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATATTGTAAGtt < 2:1575419/76‑1 (MQ=255) caccGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATATTGTAAGTTa > 2:1806106/1‑76 (MQ=255) caccGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATATTGTAAGTTa > 2:1726930/1‑76 (MQ=255) caccGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATATTGTAAGTTa > 2:117380/1‑76 (MQ=255) accGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATATTGTAAGTTAc > 2:1278535/1‑76 (MQ=255) ccGAG‑AAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATATTGTAAGTTACa > 1:1574900/1‑76 (MQ=255) aaaaaaaaaGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATATTGTAAGTTACAATaa > 1:114537/1‑76 (MQ=255) | CTAATTTTTTGAACTTTAGAAAAGCCTTTATCATCAAATCGTATTGTTTATCTTCATGCAAAGCACCCACCGAGAAAAAAAAAGGTTCACAATCATATTGGTGTATAGAGGATTTTTTAAAATCACCTAATATATTGTAAGTTACAATAA > REL606/2036292‑2036441 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |