Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,875,6251.T100.0% 191.1 / NA 48intergenic (‑59/+294)glmU/atpCbifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (27/21);  total (27/21)

TAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC  >  REL606/3875551‑3875695
                                                                           |                                                                      
tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t                                                                       >  2:1804171/1‑76 (MQ=255)
tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t                                                                       >  2:1150015/1‑76 (MQ=255)
  gCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt                                                                     >  1:1755059/1‑76 (MQ=255)
   cATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttt                                                                    <  1:4988/76‑1 (MQ=255)
   cATTATTCAACATACGCCTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttt                                                                    >  2:1627661/1‑76 (MQ=255)
      tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttttttt                                                                 <  2:531622/76‑1 (MQ=255)
              aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTATATTTTTTTTCATCGTg                                                          >  2:1561679/1‑76 (MQ=255)
              aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTg                                                          >  1:1314300/1‑76 (MQ=255)
              aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTg                                                          <  1:674536/76‑1 (MQ=255)
                aCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGaa                                                        >  1:1052701/1‑76 (MQ=255)
                aCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGaa                                                        <  2:833375/76‑1 (MQ=255)
                  gcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAAt                                                      <  1:1278603/76‑1 (MQ=255)
                   cgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATg                                                     >  2:935583/1‑76 (MQ=255)
                   cgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATg                                                     <  2:1727562/76‑1 (MQ=255)
                    gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTTATCCTGAAAttg                                                    >  1:1800035/1‑74 (MQ=255)
                      ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGAc                                                  >  1:1154412/1‑76 (MQ=255)
                      ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGAc                                                  >  1:919518/1‑76 (MQ=255)
                         gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACaga                                               <  1:773572/76‑1 (MQ=255)
                          aCTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACagag                                              >  1:1089271/1‑76 (MQ=255)
                          aCTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACagag                                              >  1:1089286/1‑76 (MQ=255)
                             gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGAt                                           >  2:322368/1‑76 (MQ=255)
                              tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATa                                          <  1:379458/76‑1 (MQ=255)
                              tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATa                                          <  1:619474/76‑1 (MQ=255)
                               aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATaa                                         <  2:583543/76‑1 (MQ=255)
                                 tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAAt                                       <  2:361048/76‑1 (MQ=255)
                                  ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAAtt                                      <  1:893312/76‑1 (MQ=255)
                                   tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTg                                     <  2:91903/76‑1 (MQ=255)
                                   tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTg                                     >  2:1080149/1‑76 (MQ=255)
                                        aCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCaa                                <  1:1738031/76‑1 (MQ=255)
                                         cGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt                               <  1:959664/76‑1 (MQ=255)
                                          gAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa                              >  1:1628032/1‑76 (MQ=255)
                                            aTTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAAc                            <  2:877700/76‑1 (MQ=255)
                                               tAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa                         >  1:477360/1‑76 (MQ=255)
                                                aaaCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt                        >  1:1568301/1‑76 (MQ=255)
                                                 aaCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt                       <  2:1513441/76‑1 (MQ=255)
                                                 aaCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt                       <  1:928215/76‑1 (MQ=255)
                                                  aCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa                      <  2:1734787/76‑1 (MQ=255)
                                                       ttCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc                 >  1:1128576/1‑76 (MQ=255)
                                                        tCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg                >  2:1492849/1‑76 (MQ=255)
                                                        tCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg                >  2:204551/1‑76 (MQ=255)
                                                         cACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGc               >  1:1184491/1‑76 (MQ=255)
                                                         cACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGc               >  1:1351414/1‑76 (MQ=255)
                                                         cACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGc               >  1:1184508/1‑76 (MQ=255)
                                                          aCCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCt              <  2:1429203/76‑1 (MQ=255)
                                                          aCCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCt              >  2:79040/1‑76 (MQ=255)
                                                            cTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa            >  1:101320/1‑76 (MQ=255)
                                                             ttGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc           >  2:325049/1‑76 (MQ=255)
                                                             ttGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc           >  1:1555517/1‑76 (MQ=255)
                                                              tGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg          >  1:823186/1‑76 (MQ=255)
                                                              tGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg          >  1:458224/1‑76 (MQ=255)
                                                               gAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGa         <  2:661253/76‑1 (MQ=255)
                                                                    aaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcg    <  1:212045/76‑1 (MQ=255)
                                                                    aaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcg    <  2:925296/76‑1 (MQ=255)
                                                                      cTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGcc  >  2:325062/1‑76 (MQ=255)
                                                                           |                                                                      
TAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC  >  REL606/3875551‑3875695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑