| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,747,903 | 0 | G | T | 31.6% | 145.9 / 67.2 | 76 | A8S (GCA→TCA) | ydiL | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (25/27); new base T (24/0); total (49/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.80e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAA > REL606/1747829‑1747975 | agGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGc > 2:704079/1‑76 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCa > 1:1052724/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:305700/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcg > 1:1657916/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcc > 1:113502/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcc > 1:1238788/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcc > 1:578410/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcc > 1:1772686/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAca > 1:585312/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:98110/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:1204946/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACt > 2:428248/1‑76 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcca > 1:1289643/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcca > 1:273253/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtt > 1:886533/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtt > 1:1491679/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACta > 1:268549/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTc > 2:646987/1‑76 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcctt > 1:1761889/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAccga > 1:456781/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAccag > 1:180439/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:1023341/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:1620024/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:134211/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:1164874/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACGcc > 1:148115/1‑76 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTcc < 1:1508334/76‑1 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCg > 2:1476789/1‑76 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCg < 1:362052/76‑1 (MQ=255) tAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGc > 2:205679/1‑76 (MQ=255) tAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGc > 2:1780982/1‑76 (MQ=255) aaTGTTATTTATATTTTATTGTTTTTTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACGccaat > 1:706834/1‑73 (MQ=255) aaTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGcc < 1:1059681/76‑1 (MQ=255) aaTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGcc > 1:551286/1‑76 (MQ=255) aTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCa > 2:1396870/1‑76 (MQ=255) aTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCa > 2:1382219/1‑76 (MQ=255) aTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCa < 1:718625/76‑1 (MQ=255) tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCat > 1:747443/1‑76 (MQ=255) tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCat > 1:747416/1‑76 (MQ=255) tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCat > 1:399418/1‑76 (MQ=255) ttatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCatat > 1:433766/1‑76 (MQ=255) tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCatatc < 1:1612474/76‑2 (MQ=255) tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtt < 1:507907/76‑1 (MQ=255) tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtt < 2:412230/76‑1 (MQ=255) atttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAttt < 2:1669681/76‑1 (MQ=255) tttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtttt < 1:97708/76‑1 (MQ=255) tttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtttt > 2:617890/1‑76 (MQ=255) tattttatttTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGcc < 1:908374/76‑1 (MQ=255) ttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa > 1:1790256/1‑76 (MQ=255) tATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGAc < 1:1757325/76‑1 (MQ=255) tATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGAc > 2:1197064/1‑76 (MQ=255) tATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGAc > 1:943130/1‑76 (MQ=255) tttttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt < 1:676733/74‑1 (MQ=255) gTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGCCTAtt > 2:1510380/1‑76 (MQ=255) tttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtg < 1:526044/76‑1 (MQ=255) tttttCATGGAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat < 1:730102/74‑1 (MQ=255) aTTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatg < 2:1118266/76‑1 (MQ=255) ttCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGaa > 1:1036214/1‑76 (MQ=255) ttCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGaa < 1:1555484/76‑1 (MQ=255) ttCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACGCCGCCATATTTTTGCCATGACTATTGATGaa < 2:1061194/76‑1 (MQ=255) cATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATg < 1:1784553/76‑1 (MQ=255) tGCAGCCATTACACGATGAACGCTTATGACCCCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcg < 1:905210/76‑1 (MQ=255) tGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcg < 1:290206/76‑1 (MQ=255) gCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcgc > 1:1422235/1‑76 (MQ=255) ttACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTgg > 2:411744/1‑76 (MQ=255) acacGATGAACGCTTATGACCTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAt < 1:1613087/76‑1 (MQ=255) acacAATAAACGCTAATCACCCCCAGGCCCTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAt < 1:1391528/76‑1 (MQ=255) acGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTg < 2:967774/76‑1 (MQ=255) acGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTg < 1:1732172/76‑1 (MQ=255) aTGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGccc < 1:454889/76‑1 (MQ=255) tGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCa < 1:101985/76‑1 (MQ=255) gAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAg < 2:1228441/76‑1 (MQ=255) gAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAg < 2:1037064/76‑1 (MQ=255) ttATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGt < 2:67510/76‑1 (MQ=255) tATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTg > 1:1633385/1‑76 (MQ=255) gAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACa < 2:166131/76‑1 (MQ=255) gAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACa > 1:1713933/1‑76 (MQ=255) cccccAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGt < 1:99592/73‑1 (MQ=255) accccAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGt < 1:1499840/73‑1 (MQ=255) aCTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGt > 2:774456/1‑76 (MQ=255) cTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTg < 1:226958/76‑1 (MQ=255) ccAGGCCCTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGaa < 1:1662714/76‑1 (MQ=255) ccAGCCCCTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGaa < 1:1578051/76‑1 (MQ=255) cAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGaaa > 2:719783/1‑76 (MQ=255) | AGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAA > REL606/1747829‑1747975 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |