Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	475,131	C→T	E59K (GAA→AAA)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	475,131	0	C	T	100.0%	163.9 / NA	44	E59K (GAA→AAA)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/22);  total (22/22)

CAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCC  >  REL606/475063‑475203                                                                     |                                                                         cAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCa                                                                   <  1:1622153/76‑1 (MQ=255)   aaTCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAg                                                                 <  1:669497/76‑1 (MQ=255)    aTCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGc                                                                >  1:1215950/1‑76 (MQ=255)        ttCAGCGAAAAGTGCAAACCGACGCCACACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTgg                                                            >  1:1442421/1‑76 (MQ=255)        ttCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTgg                                                            >  1:1740731/1‑76 (MQ=255)          cAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTa                                                          >  1:279262/1‑76 (MQ=255)           aGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTAt                                                         <  2:1040035/76‑1 (MQ=255)           aGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTAt                                                         <  2:1475779/76‑1 (MQ=255)           aGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCAAGTTTCGGGGCAAGCTTAGTAt                                                         >  2:1749096/1‑76 (MQ=255)             cGAAAAGTGCAAACCGACGCCAAACATCACCAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGCATCg                                                       >  2:1583159/1‑76 (MQ=255)             cGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCg                                                       >  2:1583164/1‑76 (MQ=255)              gAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCgg                                                      >  2:1070992/1‑76 (MQ=255)               aaaaGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGc                                                     >  1:1637440/1‑76 (MQ=255)                 aaGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGcaa                                                   >  1:619216/1‑76 (MQ=255)                    tGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCACGCTTGGGATCGGcaacaa                                                >  1:330369/1‑76 (MQ=255)                    tGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGGATCGGcaacaa                                                >  1:330384/1‑76 (MQ=255)                     gCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACaaa                                               >  2:355188/1‑76 (MQ=255)                      cAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAg                                              <  1:417174/76‑1 (MQ=255)                      cAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGATTGGTCTCGGCACCAAAg                                              >  2:98371/1‑76 (MQ=255)                         aCCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGccc                                           <  1:357368/76‑1 (MQ=255)                          ccGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGACCg                                          >  1:636725/1‑76 (MQ=255)                            gACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGa                                        >  1:1637990/1‑76 (MQ=255)                              cGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGt                                      >  1:1316748/1‑76 (MQ=255)                              cGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGt                                      >  1:1454411/1‑76 (MQ=255)                               gCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTg                                     <  1:1052436/76‑1 (MQ=255)                               gCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGGATCGGCAACAAAGCCCGGAgag                                     >  2:770335/1‑74 (MQ=255)                                 cAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGaa                                   <  2:1228709/76‑1 (MQ=255)                                  aaaCATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAAt                                  >  2:130495/1‑76 (MQ=255)                                   aaCATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATg                                 >  2:1163051/1‑76 (MQ=255)                                    aCATCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCTAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATgg                                <  2:1004721/76‑1 (MQ=255)                                      aTCAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTc                              <  2:1093385/76‑1 (MQ=255)                                        cAACAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCt                            <  2:1492716/76‑1 (MQ=255)                                           cAGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGcc                         >  2:141193/1‑76 (MQ=255)                                            aGAATGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCa                        >  1:1543700/1‑76 (MQ=255)                                               aTGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGca                     <  1:716303/76‑1 (MQ=255)                                                tGACGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGcac                    <  1:125806/76‑1 (MQ=255)                                                   cGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGANTGAATGGTCCTGCCAGCACAcc                 <  2:250385/76‑1 (MQ=255)                                                   cGCCCAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACAcc                 >  2:939485/1‑76 (MQ=255)                                                     cccAGTTCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCg               <  2:451140/76‑1 (MQ=255)                                                        aGTGCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTa            <  2:501674/76‑1 (MQ=255)                                                          ttCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAAc          <  1:173623/76‑1 (MQ=255)                                                           tCAGCCAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACa         <  1:1399127/76‑1 (MQ=255)                                                               ccAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGata     <  2:180236/76‑1 (MQ=255)                                                               ccAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGata     >  1:1645975/1‑76 (MQ=255)                                                                cAGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGatat    <  2:770242/76‑1 (MQ=255)                                                                 aGTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATc   <  1:362046/76‑1 (MQ=255)                                                                  gTTTCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATcc  <  1:580302/76‑1 (MQ=255)                                                                     |                                                                         CAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCC  >  REL606/475063‑475203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑