| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,145,985 | T→G | I41S (ATC→AGC) | rpoD → | RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,145,985 | 0 | T | G | 97.8% | 194.4 / ‑4.2 | 46 | I41S (ATC→AGC) | rpoD | RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (18/27); total (18/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
AAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGA > REL606/3145912‑3146054 | aaGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGACGATATCGTCGATTCAGATCAGAGCg > 2:1480915/1‑76 (MQ=255)aaGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCg > 1:1103286/1‑76 (MQ=255)aaGGAGCAAGGCTAGCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCg < 2:538410/76‑1 (MQ=255) aGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGa < 1:1239065/76‑1 (MQ=255) ggAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGaa < 1:802947/76‑1 (MQ=255) ggAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGaa < 1:1346199/76‑1 (MQ=255) gAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATANTCGATTCAGATCAGAGCGAAg > 2:295839/1‑76 (MQ=255) aGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGAcatc < 1:1048037/76‑1 (MQ=255) cTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACatcatc > 1:1785651/1‑76 (MQ=255) tCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCaa > 2:1367034/1‑76 (MQ=255) gACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATg > 2:1772038/1‑76 (MQ=255) gACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATg < 1:1580026/76‑1 (MQ=255) gACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATg < 1:577297/76‑1 (MQ=255) aCCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGa < 2:1147071/76‑1 (MQ=255) ccTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGAt < 2:1175545/76‑1 (MQ=255) cTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATc > 1:1059005/1‑76 (MQ=255) tATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCa < 1:928947/76‑1 (MQ=255) aTGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCaa < 1:971983/76‑1 (MQ=255) aTGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCaa < 1:604795/76‑1 (MQ=255) aTGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCaa < 1:1044005/76‑1 (MQ=255) tGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAAc > 1:428678/1‑76 (MQ=255) gCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACg < 1:1268689/76‑1 (MQ=255) ggTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATg < 2:57961/76‑1 (MQ=255) gTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATgg < 1:907669/76‑1 (MQ=255) gTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATgg > 1:882130/1‑76 (MQ=255) gTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATgg < 2:1439043/76‑1 (MQ=255) aaTGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCa > 1:1428902/1‑76 (MQ=255) aaTGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCa > 1:1816771/1‑76 (MQ=255) aaTGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCa < 1:738597/76‑1 (MQ=255) aaTGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCa < 1:621923/76‑1 (MQ=255) aaTGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCa < 1:20695/76‑1 (MQ=255) aTGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCAt > 1:306466/1‑76 (MQ=255) tGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCAtt < 2:1205591/76‑1 (MQ=255) tGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCAtt < 1:135083/76‑1 (MQ=255) tGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCAtt < 2:1741846/76‑1 (MQ=255) aTCTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGt < 2:503319/76‑1 (MQ=255) cTGCCGGAAGATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGa > 1:523424/1‑76 (MQ=255) gATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGaagaag > 1:570915/1‑76 (MQ=255) gATATCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGaagaag < 1:1362961/76‑1 (MQ=255) tatCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCa > 2:1133158/1‑76 (MQ=255) tatCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCa < 1:1479132/76‑1 (MQ=255) atCGTCGATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCAc > 2:1394846/1‑76 (MQ=255) gATTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATg < 2:1312170/76‑1 (MQ=255) aTTCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGc < 2:1731634/76‑1 (MQ=255) ttCAGATCAGAGCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGcc > 2:1171406/1‑76 (MQ=255) tcagatcagaGCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCg > 2:1608293/1‑76 (MQ=255) cagatcagagCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCga < 1:1367034/76‑1 (MQ=255) cagatcagagCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCga > 1:840408/1‑76 (MQ=255) cagatcagagCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCga < 1:622549/76‑1 (MQ=255) cagatcagagCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCga < 1:596308/76‑1 (MQ=255) gatcagagcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCgatg > 2:931198/1‑76 (MQ=255) atcagagcGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCgatga < 2:529369/76‑1 (MQ=255) | AAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGA > REL606/3145912‑3146054 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |