Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,414,656	A→C	*211S (TAA→TCA)	crp →	DNA‑binding transcriptional dual regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,414,656	0	A	C	100.0%	299.2 / NA	71	*211S (TAA→TCA)	crp	DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (40/31);  total (40/31)

ATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTAT  >  REL606/3414583‑3414728                                                                          |                                                                         aTTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCAt                                                                        <  1:1337806/76‑1 (MQ=255)  ttCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATc                                                                       >  2:108205/1‑76 (MQ=255)      gAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGt                                                                   >  1:1512075/1‑76 (MQ=255)      gAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGt                                                                   >  2:343912/1‑76 (MQ=255)      gAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGt                                                                   >  2:1586923/1‑76 (MQ=255)       aaGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTc                                                                  <  2:746712/76‑1 (MQ=255)         gATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCgg                                                                >  2:1381077/1‑76 (MQ=255)          aTGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGa                                                               >  2:1343931/1‑76 (MQ=255)          aTGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGa                                                               <  2:219728/76‑1 (MQ=255)           tGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAg                                                              >  2:1569735/1‑76 (MQ=255)             cTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTg                                                            >  2:1753331/1‑76 (MQ=255)              tGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTgg                                                           >  1:1296756/1‑76 (MQ=255)              tGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTgg                                                           >  2:1219593/1‑76 (MQ=255)                 aaGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGgcgc                                                        >  1:871839/1‑76 (MQ=255)                    aTCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGtt                                                     <  1:983836/76‑1 (MQ=255)                     tCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTa                                                    <  1:1631224/76‑1 (MQ=255)                      cAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTAc                                                   <  1:1441300/76‑1 (MQ=255)                           ccTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGt                                              >  2:569209/1‑76 (MQ=255)                           ccTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGt                                              >  1:1645959/1‑76 (MQ=255)                            cTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTa                                             <  2:871816/76‑1 (MQ=255)                             tGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGCGTGGCGCGTTACCTGGCAg                                            >  2:1562959/1‑76 (MQ=255)                             tGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAg                                            <  2:986848/76‑1 (MQ=255)                             tGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAg                                            <  1:743704/76‑1 (MQ=255)                             tGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAg                                            <  1:563086/76‑1 (MQ=255)                               aTCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAgcg                                          >  2:701155/1‑76 (MQ=255)                               aTCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAgcg                                          >  2:1627785/1‑76 (MQ=255)                                tctcCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAgcgc                                         >  2:384648/1‑76 (MQ=255)                                 ctcCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAgcgcg                                        >  2:18956/1‑76 (MQ=255)                                   ccGCCCACGGTAAAACCGTCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGcc                                      <  1:273198/76‑1 (MQ=255)                                   ccGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGcc                                      >  2:107660/1‑76 (MQ=255)                                   ccGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGcc                                      >  2:814662/1‑76 (MQ=255)                                   ccGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGcc                                      >  2:1310626/1‑76 (MQ=255)                                   ccGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGcc                                      >  2:1753953/1‑76 (MQ=255)                                      cacaCGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCAtt                                   >  2:624690/1‑76 (MQ=255)                                       acacGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCAttt                                  <  2:545495/76‑1 (MQ=255)                                        cacGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCAtttt                                 <  1:384648/76‑1 (MQ=255)                                        cacGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCAtttt                                 <  2:1664743/76‑1 (MQ=255)                                        cacGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCAtttt                                 <  2:1764299/76‑1 (MQ=255)                                        cacGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCAtttt                                 <  2:1813828/76‑1 (MQ=255)                                         acGGTAAAACCATCGTCGTTTACGGCACTCTTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTg                                <  2:876315/76‑1 (MQ=255)                                         acGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTg                                <  2:353967/76‑1 (MQ=255)                                         acGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTg                                <  2:1584664/76‑1 (MQ=255)                                          cGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTCGCGCGCCCTTTTGt                               >  1:1659183/1‑76 (MQ=255)                                          cGGTAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGCTCCCTGGGAGCGGGCCATTTTGt                               >  1:1572582/1‑76 (MQ=255)                                             tAAAACCATCGTCGTTTACGGCACTCGTTCATCCCGCCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTc                            <  2:1267689/76‑1 (MQ=255)                                              aaaaCCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGGTTCCTGGGAGCGCGCCCCTtttttttc                           >  1:962108/1‑74 (MQ=255)                                              aaaaCCATCGTCGTTTACGGCACTCGTTCATCCCGTCAGAGTGGCGCGTAACCTNGTAGCGCGCCATTTTGTTTcc                           >  2:289840/1‑76 (MQ=255)                                               aaaCCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTccc                          >  2:1306506/1‑76 (MQ=255)                                               aaaCCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTccc                          >  1:1674132/1‑76 (MQ=255)                                                aaCCATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTcccc                         <  1:1126111/76‑1 (MQ=255)                                                  ccATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCg                       <  2:518701/76‑1 (MQ=255)                                                   cATCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGAAGCGCGCCATTTTGTTTCCCTCgt                      >  2:1381200/1‑75 (MQ=255)                                                    aTCGTCGTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGAt                     >  2:1004263/1‑76 (MQ=255)                                                     tcgtcgTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGAtg                    <  2:1562646/76‑1 (MQ=255)                                                     tcgtcgTTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGAtg                    <  2:830813/76‑1 (MQ=255)                                                      cgtcgtTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCACCGAtgt                   >  2:1448208/1‑76 (MQ=255)                                                       gtcgtTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCCTTTTGTTTCCCCCGAtgtg                  >  2:1115207/1‑76 (MQ=255)                                                       gtcgtTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGAtgtg                  >  2:1307300/1‑76 (MQ=255)                                                       gtcgtTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGAtgtg                  >  2:589809/1‑76 (MQ=255)                                                        tcgtGTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTgg                 <  1:485972/76‑1 (MQ=255)                                                         cgtTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGgc                <  2:226665/76‑1 (MQ=255)                                                         cgtTTACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGgc                >  1:1358834/1‑76 (MQ=255)                                                           tttACGGCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCCTTTTGTTTCCCCCGATGTGgcgc              >  1:1074696/1‑76 (MQ=255)                                                                 gCACTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTg        <  2:1578751/76‑1 (MQ=255)                                                                   aCTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTTCCCCGATGTGGGGCAGACTggt      >  1:905479/1‑74 (MQ=255)                                                                    cTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGAtt     <  1:776643/76‑1 (MQ=255)                                                                    cTCGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGAtt     <  2:1401447/76‑1 (MQ=255)                                                                      cGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGCCTGATTTa   >  2:1122524/1‑76 (MQ=255)                                                                      cGTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTa   <  2:1688456/76‑1 (MQ=255)                                                                       gTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTAt  >  2:432889/1‑76 (MQ=255)                                                                       gTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTAt  >  2:863940/1‑76 (MQ=255)                                                                       gTTCATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTAt  <  2:337841/76‑1 (MQ=255)                                                                          |                                                                         ATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTAT  >  REL606/3414583‑3414728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑