Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,497,167	A→G	V290A (GTT→GCT)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,497,167	0	A	G	100.0%	161.3 / NA	41	V290A (GTT→GCT)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/17);  total (24/17)

TTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCG  >  REL606/3497094‑3497237                                                                          |                                                                       tttATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGcc                                                                      >  2:1366414/1‑76 (MQ=255)  ttATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGGGGAAGCCa                                                                     >  1:1245814/1‑76 (MQ=255)    aTCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAgg                                                                   >  1:1110305/1‑76 (MQ=255)    aTCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAgg                                                                   <  2:841399/76‑1 (MQ=255)       cGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTACAATGTCCTGCATGGTCGAGGAAGCCAGGAAg                                                                >  1:547872/1‑76 (MQ=255)       cGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAg                                                                <  2:1027882/76‑1 (MQ=255)          cAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTAt                                                             <  1:786631/76‑1 (MQ=255)          cAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTAt                                                             >  2:1538237/1‑76 (MQ=255)           aGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTTAAATGTCCTGAATGGGTGGGGGAGCCCGGGAGTAtt                                                            >  1:193853/1‑76 (MQ=255)            ggTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTc                                                           <  2:1724683/76‑1 (MQ=255)             gTTATCGTAGGTTTTATGCAACTGATAATGGCGGTTTAAAATTTCCTGAATGGCCGAGGGAGCCAGGAAGTTTTct                                                          >  1:1770547/1‑75 (MQ=255)              ttATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGGATTCCt                                                         >  1:601770/1‑76 (MQ=255)               tATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTg                                                        <  2:1451508/76‑1 (MQ=255)               tATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTg                                                        <  2:343773/76‑1 (MQ=255)                aTCGTAGGTTTTATGCAACTGATACTGGCGGCTTAAAATGGTCTGAATGGTCGAGGGAGCCCGGAAGTATTCCTGa                                                       >  1:694924/1‑76 (MQ=255)                aTCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGa                                                       >  1:700805/1‑76 (MQ=255)                   gTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGc                                                    >  2:46993/1‑76 (MQ=255)                    tAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCa                                                   >  2:1591856/1‑76 (MQ=255)                     aGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAg                                                  >  2:1722220/1‑76 (MQ=255)                     aGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAg                                                  <  1:1191229/76‑1 (MQ=255)                       gTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTAATCCTTacgcacgc                                                >  2:908603/1‑76 (MQ=255)                          ttATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGCGGAAGGCCGGAAGGAGTCCGGGCGCAGgcgcc                                             >  1:216413/1‑75 (MQ=255)                          ttATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCa                                             <  1:1521204/76‑1 (MQ=255)                                aaCTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCAc                                       >  2:898260/1‑76 (MQ=255)                                aaCTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCAc                                       <  2:717557/76‑1 (MQ=255)                                aaCTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCAc                                       >  2:1548136/1‑76 (MQ=255)                                 aCTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACg                                      <  1:1112234/76‑1 (MQ=255)                                    gATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGGGCAGCTCACGccc                                   >  1:66865/1‑76 (MQ=255)                                    gATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGccc                                   <  1:1642585/76‑1 (MQ=255)                                     aTAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACccccc                                  >  2:492711/1‑75 (MQ=255)                                      tAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCgg                                 <  2:860693/76‑1 (MQ=255)                                         tGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATCCCTGACGCAGGCGCAGCTCACGCCCGGAGt                              >  1:1485574/1‑76 (MQ=255)                                          ggcggcTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTa                             <  2:478392/76‑1 (MQ=255)                                           gcggcTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAg                            >  2:688650/1‑76 (MQ=255)                                                 tAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAg                      >  1:1005572/1‑76 (MQ=255)                                                 tAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAg                      <  1:35854/76‑1 (MQ=255)                                                  aaaaTGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGt                     >  2:189149/1‑76 (MQ=255)                                                   aaaTGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTc                    >  2:225809/1‑76 (MQ=255)                                                    aaTGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCa                   <  2:1689420/76‑1 (MQ=255)                                                    aaTGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCa                   >  2:358570/1‑76 (MQ=255)                                                        tCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCg               >  1:397576/1‑76 (MQ=255)                                                            gAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATa           >  1:701241/1‑76 (MQ=255)                                                              aTGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACa         >  1:365561/1‑76 (MQ=255)                                                               tGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAg        >  2:1748347/1‑76 (MQ=255)                                                                  tCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAc     >  1:977018/1‑76 (MQ=255)                                                                   cGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAcg    >  2:1136916/1‑76 (MQ=255)                                                                   cGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAcg    <  1:615552/76‑1 (MQ=255)                                                                   cGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAcg    <  1:928044/76‑1 (MQ=255)                                                                    gAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAcgc   >  2:355856/1‑76 (MQ=255)                                                                    gAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGAGGAGTCATCCGGATACAGTAcgc   >  2:229121/1‑76 (MQ=255)                                                                    gAGGAAGCCAGGAAGTATTCCTGACGCAGGCGAAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAcgc   >  2:736032/1‑76 (MQ=255)                                                                     aGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAcgcg  >  1:1362878/1‑76 (MQ=255)                                                                          |                                                                       TTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCG  >  REL606/3497094‑3497237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑