BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,165,612	A→C	T218P (ACC→CCC)	rpoC →	DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,165,612	0	A	C	100.0%	274.7 / NA	64	T218P (ACC→CCC)	rpoC	DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (31/33); total (31/33)

TCTGGAGCAAGAGTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTT  >  REL606/4165539‑4165683

tCTGGAGCAAGAGTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGccc                                                                       <  1:975701/76‑1 (MQ=255)

  tGGAGCAAGAGTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCaa                                                                     <  1:779363/76‑1 (MQ=255)

   ggAGCAAGAGTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAg                                                                    <  2:1687525/76‑1 (MQ=255)

      gCAAGAGTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGt                                                                 >  2:1240711/1‑76 (MQ=255)

      gCAAGAGTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGt                                                                 >  2:363241/1‑76 (MQ=255)

      gCAAGAGTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGt                                                                 <  1:44568/76‑1 (MQ=255)

      gCAAGAGTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGt                                                                 >  2:535204/1‑76 (MQ=255)

       cAAGAGTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTa                                                                >  1:159964/1‑76 (MQ=255)

       cAAGAGTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTa                                                                <  1:359989/76‑1 (MQ=255)

       cAAGAGTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTa                                                                >  2:1556721/1‑76 (MQ=255)

       cAAGAGTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTa                                                                >  1:772153/1‑76 (MQ=255)

        aaGAGTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTAt                                                               >  2:440397/1‑76 (MQ=255)

         agagTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATc                                                              >  2:1052063/1‑76 (MQ=255)

           agTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCaa                                                            >  2:570344/1‑76 (MQ=255)

           agTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCaa                                                            <  1:909499/76‑1 (MQ=255)

           agTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCaa                                                            <  2:197212/76‑1 (MQ=255)

             tGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAAc                                                          <  1:1581085/76‑1 (MQ=255)

              gCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACt                                                         <  2:941742/76‑1 (MQ=255)

               cGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTg                                                        <  1:682351/76‑1 (MQ=255)

               cGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTg                                                        >  2:170259/1‑76 (MQ=255)

                gAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGt                                                       >  1:995511/1‑76 (MQ=255)

                 aaCAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGtt                                                      >  2:621331/1‑76 (MQ=255)

                  aCAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTg                                                     <  2:828543/76‑1 (MQ=255)

                     gTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGaa                                                  <  1:766947/76‑1 (MQ=255)

                     gTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGaa                                                  <  2:122478/76‑1 (MQ=255)

                      ttGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAg                                                 <  2:1004259/76‑1 (MQ=255)

                       tGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGc                                                >  1:71766/1‑76 (MQ=255)

                          gTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGcgtt                                             <  2:928271/76‑1 (MQ=255)

                            gAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGcgttcg                                           >  2:397897/1‑76 (MQ=255)

                            gAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGcgttcg                                           >  2:1041889/1‑76 (MQ=255)

                                agCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCa                                       <  2:1548860/76‑1 (MQ=255)

                                    gAACGAAACCAACGCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCt                                   <  2:31204/76‑1 (MQ=255)

                                       cGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGt                                <  1:1515480/76‑1 (MQ=255)

                                        gAAACCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTa                               >  1:250046/1‑76 (MQ=255)

                                         aaaCCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTaa                              >  2:957022/1‑76 (MQ=255)

                                         aaaCCAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTaa                              <  1:1588609/76‑1 (MQ=255)

                                             cAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACaaa                          >  2:1493221/1‑76 (MQ=255)

                                             cAACTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACaaa                          >  2:1398976/1‑76 (MQ=255)

                                              aaCTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAAc                         <  2:545700/76‑1 (MQ=255)

                                               aCTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAAcc                        <  2:1427666/76‑1 (MQ=255)

                                               aCTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAAcc                        <  2:1024575/76‑1 (MQ=255)

                                               aCTCCGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAAcc                        <  1:907247/76‑1 (MQ=255)

                                                  ccGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCaga                     >  2:944282/1‑76 (MQ=255)

                                                  ccGAAACCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCaga                     >  2:602449/1‑76 (MQ=255)

                                                     aaaCCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTg                  >  1:1435567/1‑76 (MQ=255)

                                                     aaaCCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTg                  >  2:569882/1‑76 (MQ=255)

                                                     aaaCCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTg                  >  2:1000763/1‑76 (MQ=255)

                                                      aaCCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTgg                 <  2:369185/76‑1 (MQ=255)

                                                      aaCCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTgg                 >  2:15514/1‑76 (MQ=255)

                                                      aaCCAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTgg                 <  2:167639/76‑1 (MQ=255)

                                                        ccAAGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGgat               <  1:192790/76‑1 (MQ=255)

                                                           atcGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGgatgat            <  2:627123/74‑1 (MQ=255)

                                                           aGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGgatgat            <  2:1210636/76‑1 (MQ=255)

                                                           aGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGgatgat            >  1:1542757/1‑76 (MQ=255)

                                                           aGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGgatgat            <  1:505979/76‑1 (MQ=255)

                                                           aGCGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGgatgat            <  1:1716717/76‑1 (MQ=255)

                                                             cGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATcc          >  1:1034644/1‑76 (MQ=255)

                                                             cGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATcc          >  2:1694352/1‑76 (MQ=255)

                                                             cGTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATcc          >  1:551340/1‑76 (MQ=255)

                                                              gTAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCt         <  2:515252/76‑1 (MQ=255)

                                                               tAAAAAGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTg        <  1:413463/76‑1 (MQ=255)

                                                               tAAAAAACTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTg        <  2:1609355/76‑1 (MQ=255)

                                                                aaaaaGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGa       >  2:1122020/1‑76 (MQ=255)

                                                                  aaaGCTGCCCAAGCTTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGAcc     >  1:1543849/1‑76 (MQ=255)

                                                                   aaGCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCg    <  1:747268/76‑1 (MQ=255)

                                                                     gCTGCCCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGtt  <  2:151540/76‑1 (MQ=255)

TCTGGAGCAAGAGTGCGAACAGTTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGTTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTT  >  REL606/4165539‑4165683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`