| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,997,463 | (A)9→8 | coding (131/903 nt) | ECB_02798 → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,997,455 | 0 | A | . | 97.6% | 194.9 / ‑0.9 | 42 | coding (123/903 nt) | ECB_02798 | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (24/17); total (24/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TTATCTTGGAGAGCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATAAAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAAATAATTTCTGGTATGC > REL606/2997380‑2997525 | ttatCTTGGAGAGCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATa > 2:1512698/1‑76 (MQ=255) tatCTTGGAGAGCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATaa < 2:972463/76‑1 (MQ=255) tatCTTGGAGAGCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATaa < 2:1297245/76‑1 (MQ=255) atCTTGGAGAGCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATaaa > 1:814165/1‑76 (MQ=255) atCTTGGAGAGCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATaaa < 2:1133428/76‑1 (MQ=255) tCTTGGAGAGCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATaaaa < 1:1119402/76‑1 (MQ=255) cTTGGAGAGCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATaaaaa > 1:1198676/1‑76 (MQ=255) cTTGGAGAGCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATaaaaa > 2:1617304/1‑76 (MQ=255) tGGAGAGCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAAGGTTTTATCGCTTACATaaaaaaa > 2:121145/1‑76 (MQ=255) tGGAGAGCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATaaaaaaa < 2:1611966/76‑1 (MQ=255) tGGAGAGCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATaaaaaaa > 2:1611222/1‑76 (MQ=255) tGGAGAGCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATaaaaaaa > 1:1641621/1‑76 (MQ=255) agagCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATaaaaaaaaca > 2:1527090/1‑74 (MQ=255) gagCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATaaaaaaaacag < 2:885093/76‑4 (MQ=255) gCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACagag > 2:990820/1‑76 (MQ=255) gTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGaa < 1:63169/76‑1 (MQ=255) aaTATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGaaaa < 2:1669344/76‑1 (MQ=255) aaTATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGaaaa > 1:85504/1‑76 (MQ=255) atCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCt < 2:1198676/76‑1 (MQ=255) ccGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAAc > 1:1469594/1‑76 (MQ=255) gTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACAt > 2:996246/1‑76 (MQ=255) gTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACAt < 2:1373323/76‑1 (MQ=255) cccTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACtt > 1:149596/1‑76 (MQ=255) ccTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACttt < 2:1182250/76‑1 (MQ=255) cTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTc < 2:1689899/76‑1 (MQ=255) cTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTc < 1:655155/76‑1 (MQ=255) tAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAg > 1:1589303/1‑76 (MQ=255) aaTGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGa > 1:1051989/1‑76 (MQ=255) tGTTTATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGAtt < 1:820335/76‑1 (MQ=255) tttATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGAtttt > 1:1051045/1‑76 (MQ=255) tttATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGAtttt > 2:398607/1‑76 (MQ=255) ttATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTttg < 1:1024013/76‑1 (MQ=255) ttATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTttg > 2:204727/1‑76 (MQ=255) ttATGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTttg < 1:1456857/76‑1 (MQ=255) tGATCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTttgaac > 1:1431288/1‑76 (MQ=255) aTCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTttgaactt > 2:808411/1‑76 (MQ=255) aTCCCCCGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTttgaactt > 2:1325504/1‑76 (MQ=255) ccGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACa < 1:505963/76‑1 (MQ=255) cGGAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCATACTTTCAGATTTTGAACTTGAACaa > 2:1103363/1‑76 (MQ=255) ggAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACaaa < 2:869740/76‑1 (MQ=255) gAACGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAaat < 1:454754/76‑1 (MQ=255) aaCGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAaata > 2:1093663/1‑76 (MQ=255) aaCGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAaata > 2:1093670/1‑76 (MQ=255) cGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAaataat < 2:94997/76‑1 (MQ=255) cGTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTGTCAGNTTTTGAACTTGAACAaataat > 2:268013/1‑76 (MQ=255) gTTTTATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGACCATCACACTTTCAGATTTTGACCTTGAACAAATAAtt > 1:695577/1‑76 (MQ=255) ttttATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAAATAAttt > 1:800774/1‑76 (MQ=255) tttATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAAATAATTTc > 1:124976/1‑76 (MQ=255) tttATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAAATAATTTc > 1:1582442/1‑76 (MQ=255) ttATCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAAATAATTTCt < 2:1641621/76‑1 (MQ=255) aTCGCTTACATAAAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAAATAATTTCTg < 2:1448106/76‑1 (MQ=255) tCGCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAAATAATTTCTGGt > 2:1217125/1‑76 (MQ=255) gCTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAAATAATTTCTGGTAt < 1:1666840/76‑1 (MQ=255) cTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAAATAATTTCTGGTATg > 2:338518/1‑76 (MQ=255) cTTACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAAATAATTTCTGGTATg > 1:481122/1‑76 (MQ=255) ttACAT‑AAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAAATAATTTCTGGTATGc < 1:1145983/76‑1 (MQ=255) | TTATCTTGGAGAGCGTAATATCTCCGTACTGCCCCTTAATGTTTATGATCCCCCGGAACGTTTTATCGCTTACATAAAAAAAAACAGAGAAAATCTGAACATCACACTTTCAGATTTTGAACTTGAACAAATAATTTCTGGTATGC > REL606/2997380‑2997525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |