Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	3,326,715	+AAT	coding (52/489 nt)	mreD ←	cell wall structural complex MreBCD transmembrane component MreD

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 3326712	0 (0.000)	51 (1.000) +AAT	40/138	0.2	100%	coding (55/489 nt)	mreD	cell wall structural complex MreBCD transmembrane component MreD
	?	REL606	3326713 =	0 (0.000)					coding (54/489 nt)	mreD	cell wall structural complex MreBCD transmembrane component MreD

CAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/3326638‑3326712 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACG  >  REL606/3326713‑3326786                                                                            |||                                                                           CAAGATGAGTAACACCCAGTTTGGCCAGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCA                                                                              <  1:947742/76‑1  AAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAA                                                                             >  2:1427535/1‑76    GATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATA                                                                           <  1:1378207/76‑1       GAGTACCACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATG                                                                        <  2:975050/76‑1        AGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGA                                                                       <  2:117557/76‑1         GTAACCCCCAGTTTGGCCGGAAAACAATCAGGTTAGCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAG                                                                      <  1:628370/76‑1         GTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAG                                                                      <  2:1037306/76‑1          TAACACCCAGTTGGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGG                                                                     <  1:1454339/76‑1              cCCCAGTTTGGCCGGAAAACAATCAGTTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAG                                                                 <  1:913440/75‑1               CCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGA                                                                >  1:988363/1‑76                    ttgGGCGGGAAAAAAATCAGGTTACCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCC                                                           <  1:775976/73‑1                    TTTGGCCGGAAAACAATCAGTTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCC                                                           <  2:216103/76‑1                    TTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCC                                                           <  2:216088/76‑1                      TGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAG                                                         >  2:938069/1‑76                      TGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAG                                                         >  1:526003/1‑76                      TGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAACAACAGCGCAATAATGAGGAAAGAGAGCCAG                                                         >  1:990863/1‑76                         CCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATT                                                      <  2:721896/76‑1                             AAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCC                                                  <  1:1207934/76‑1                             AAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCC                                                  >  1:1224732/1‑76                               AACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAG                                                >  2:151530/1‑76                                   ATCGGGTTATCCGGCGAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTC                                            <  1:1476496/76‑1                                   ATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTC                                            >  1:394497/1‑76                                    TCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCC                                           <  2:346768/76‑1                                    TCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCC                                           >  1:331680/1‑76                                     CAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCC                                          >  2:820533/1‑76                                      AGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCT                                         <  2:1379656/76‑1                                         TTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGC                                      >  2:801495/1‑76                                           ATCCGGCCAGGGCATGATTTGNCGCAGCAGCGCAATAACGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTA                                    <  2:272277/76‑1                                           ATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTA                                    <  1:1676146/76‑1                                              CGGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGA                                 >  1:873611/1‑76                                               GGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGAT                                <  2:443712/76‑1                                               GGCCAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGAT                                <  2:318055/76‑1                                                 cgAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAG                              <  1:576157/74‑1                                                  CAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGC                             >  2:943375/1‑76                                                  CAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGC                             >  2:1046371/1‑76                                                  CAGGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGC                             >  2:1235843/1‑76                                                    GGGCATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCCACGACAGCTC                           >  1:659679/1‑76                                                       CATGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCC                        >  2:1563424/1‑76                                                         TGATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCAC                      >  1:1321424/1‑76                                                          GATTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACT                     <  2:1268401/76‑1                                                            TTTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTAT                   >  2:1334556/1‑76                                                             TTGCAGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATT                  <  2:1163949/76‑1                                                                 AGCAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCC              <  1:225648/76‑1                                                                   CAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTC            >  2:719285/1‑76                                                                   CAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTC            >  2:85141/1‑76                                                                   CAACAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTC            <  2:1274137/76‑1                                                                    AACAGCGCAATAATGAGGAAAGNGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCC           >  2:231091/1‑76                                                                      CAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCG         <  2:1681882/76‑1                                                                      CAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCG         >  2:1054455/1‑76                                                                      CAGCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCG         >  2:1445194/1‑76                                                                        GCGCAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGC       <  2:364486/76‑1                                                                         CGCAATAATGAGGAAAGAGACCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCG      <  1:237746/76‑1                                                                           CAATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCA    >  1:105015/1‑76                                                                             ATAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACG  <  1:484245/76‑1                                                                            |||                                                                           CAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/3326638‑3326712 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACG  >  REL606/3326713‑3326786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.