| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,747,903 | 0 | G | T | 22.9% | 208.7 / 40.9 | 70 | A8S (GCA→TCA) | ydiL | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (30/24); new base T (16/0); total (46/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.94e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAG > REL606/1747828‑1747976 | cagGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAgg > 1:747135/1‑76 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCa > 1:1464826/1‑76 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCa > 2:1177945/1‑76 (MQ=255) ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCa < 1:1254252/76‑1 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:1540152/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:495869/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:67701/1‑76 (MQ=255) ntGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcc > 1:1228/2‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcc > 1:702352/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcgc > 1:245824/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAccg > 1:303104/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcca > 1:1402866/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcat > 1:560414/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtt > 1:451184/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTc > 1:870463/1‑76 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTc > 1:37084/1‑76 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcggc > 1:625980/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcgat > 1:1590807/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTcc < 1:524851/76‑1 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtgac > 1:435200/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtagc > 1:157458/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCg > 1:1015901/1‑76 (MQ=255) tAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACGccaa > 1:122650/1‑74 (MQ=255) aaTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGcc > 1:688907/1‑76 (MQ=255) aaTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGcc > 1:1531038/1‑76 (MQ=255) aaTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGcc > 1:772370/1‑76 (MQ=255) tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCat < 1:1162376/76‑1 (MQ=255) tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCat < 1:627676/76‑1 (MQ=255) tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCat < 2:1536628/76‑1 (MQ=255) tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtt > 1:988449/1‑76 (MQ=255) tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtt > 2:19495/1‑76 (MQ=255) tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtt < 2:1605687/76‑1 (MQ=255) atttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACTTAGGCatatcg > 1:870510/1‑74 (MQ=255) atttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAttt < 2:907035/76‑1 (MQ=255) tttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtttt > 2:1258445/1‑76 (MQ=255) tttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtttt > 2:1201885/1‑76 (MQ=255) ttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAttttt > 1:321977/1‑76 (MQ=255) tataTTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTg > 1:541475/1‑76 (MQ=255) tataTTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTg > 1:447803/1‑76 (MQ=255) atatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGc > 1:1112686/1‑76 (MQ=255) atatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGc > 1:917213/1‑76 (MQ=255) tatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGcc > 1:33242/1‑76 (MQ=255) atTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCa > 2:563170/1‑76 (MQ=255) ttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa > 1:769104/1‑76 (MQ=255) tATTGTTTATTTCATGCAGCCANTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGAc > 2:236349/1‑76 (MQ=255) ttGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTa > 2:823293/1‑76 (MQ=255) tttttATTCCATGCAGCCATTACACGATAAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt < 1:458342/74‑1 (MQ=255) tGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt > 1:1308153/1‑76 (MQ=255) gTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAtt < 2:257821/76‑1 (MQ=255) tttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtg < 2:512633/76‑1 (MQ=255) ttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga > 2:1206721/1‑76 (MQ=255) tATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat < 2:14430/76‑1 (MQ=255) tttCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatga < 2:1388550/76‑1 (MQ=255) tttCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatga < 1:428549/76‑1 (MQ=255) aTGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgc > 1:949677/1‑76 (MQ=255) gCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcgc > 1:1229056/1‑76 (MQ=255) cAGCCATTACACGATGACCGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGcgcg < 1:1572105/76‑1 (MQ=255) aGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGa > 1:1315490/1‑76 (MQ=255) cATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCt > 1:47971/1‑76 (MQ=255) cGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGc < 2:1380446/76‑1 (MQ=255) aTGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGccc < 1:1252359/76‑1 (MQ=255) tGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCa < 2:1313724/76‑1 (MQ=255) gAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAg < 2:581139/76‑1 (MQ=255) gAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAg > 1:1405348/1‑76 (MQ=255) aaCGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGa < 2:220925/76‑1 (MQ=255) cGCTTATGACCCCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGAcc < 1:176862/76‑1 (MQ=255) cGCTTATGACCCCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGAcc < 1:12649/76‑1 (MQ=255) cGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGAcc < 2:357163/76‑1 (MQ=255) cGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGAcc < 1:863987/76‑1 (MQ=255) ttATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGt > 2:170846/1‑76 (MQ=255) tGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGAc < 1:548825/76‑1 (MQ=255) tGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGAc < 1:1400906/76‑1 (MQ=255) gaccTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACa < 1:703667/73‑1 (MQ=255) aCTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGt < 2:1584111/76‑1 (MQ=255) tCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGa < 2:183224/76‑1 (MQ=255) cAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGaaa > 1:1265053/1‑76 (MQ=255) aGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAg < 2:884105/76‑1 (MQ=255) | CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAG > REL606/1747828‑1747976 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |