| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,168,904 | C→T | A1315V (GCA→GTA) | rpoC → | DNA‑directed RNA polymerase subunit beta' |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,168,904 | 0 | C | T | 97.6% | 149.6 / NA | 41 | A1315V (GCA→GTA) | rpoC | DNA‑directed RNA polymerase subunit beta' |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (21/19); minor base G (1/0); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAATTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCG > REL606/4168831‑4168974 | gAATTGGAAGCGAACGGCAAANTGGGTGCAACTTACTCTCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTaa < 2:269756/76‑1 (MQ=255)gAATTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTaa < 2:710485/76‑1 (MQ=255)gAATTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTaa < 1:36506/76‑1 (MQ=255) aaTTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAAc < 1:1546846/76‑1 (MQ=255) aTTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAAcc > 2:998464/1‑76 (MQ=255) aTTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAAcc > 2:1119511/1‑76 (MQ=255) ttGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCg < 1:322338/76‑1 (MQ=255) ttGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTATGGTAACCg > 2:720967/1‑76 (MQ=255) ggAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAg < 2:382350/76‑1 (MQ=255) gAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGt < 2:890631/76‑1 (MQ=255) cGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCtt < 2:22649/76‑1 (MQ=255) aaCGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCa < 1:40822/76‑1 (MQ=255) cAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTcc < 1:1126395/76‑1 (MQ=255) cAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTcc < 2:1281388/76‑1 (MQ=255) aaaGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCcg < 1:1570846/76‑1 (MQ=255) aaaGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCcg > 2:1565442/1‑76 (MQ=255) tGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGc > 1:223933/1‑76 (MQ=255) aaCTTACTCCCGCGATCTGCTGGGTATCACTAAAGCGTCTCTGGGAACCGAGTCGTTCATCTCCGTGGCATCGGTc > 1:1524968/1‑76 (MQ=255) aaCTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTc > 2:1202060/1‑76 (MQ=255) tACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAgg < 1:987292/76‑1 (MQ=255) cTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGgag > 1:852634/1‑76 (MQ=255) cccGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGAc > 1:847569/1‑76 (MQ=255) cccGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGAc > 2:266107/1‑76 (MQ=255) ccGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGAcc > 1:1570978/1‑76 (MQ=255) cgcgATCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCa > 2:1660973/1‑76 (MQ=255) aTCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTcg > 1:1522054/1‑76 (MQ=255) aTCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTcg > 2:623135/1‑76 (MQ=255) aTCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTcg < 2:411647/76‑1 (MQ=255) aTCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTcg < 2:1320002/76‑1 (MQ=255) tCTGCTGGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTcgc > 1:709292/1‑76 (MQ=255) tgGGTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCt > 2:1612187/1‑76 (MQ=255) gTATCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGAc < 1:1367553/76‑1 (MQ=255) aTCACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCg > 2:834623/1‑76 (MQ=255) tCACCATAGCGTCTCTGGTAAACGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGa < 2:702623/76‑1 (MQ=255) cACCAAAGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGaa > 1:702818/1‑76 (MQ=255) aaaGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAagcag < 2:1396216/76‑1 (MQ=255) aGCGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGcc > 1:1056989/1‑76 (MQ=255) cGTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGt > 2:116404/1‑76 (MQ=255) gTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGtt < 2:356449/76‑1 (MQ=255) gTCTCTGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGtt < 2:1389564/76‑1 (MQ=255) ctctGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGc > 1:308661/1‑76 (MQ=255) ctctGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGc < 1:379466/76‑1 (MQ=255) tctGGTAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCg > 2:468122/1‑76 (MQ=255) | GAATTGGAAGCGAACGGCAAAGTGGGTGCAACTTACTCCCGCGATCTGCTGGGTATCACCAAAGCGTCTCTGGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCG > REL606/4168831‑4168974 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |