Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	4,524,522	IS186 (+) +6 bp	coding (494‑499/549 nt)	fimA →	major type 1 subunit fimbrin (pilin)

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2772854 =	NA (NA)	68 (1.150)	44/144	0.2	98.4%	noncoding (1/1343 nt)	IS186	repeat region
	?	REL606	= 4524527	1 (0.020)					coding (499/549 nt)	fimA	major type 1 subunit fimbrin (pilin)
*	?	REL606	= 2774196	NA (NA)	78 (1.320)	50/144	0.1	98.6%	noncoding (1343/1343 nt)	IS186	repeat region
	?	REL606	4524522 =	1 (0.020)					coding (494/549 nt)	fimA	major type 1 subunit fimbrin (pilin)

ACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2774119‑2774196 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGA  >  REL606/4524522‑4524595                                                                                                                                                          ACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATG                                                                              <  1:1324492/76‑1 ACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATG                                                                              <  2:495984/76‑1  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                             >  1:1736475/1‑76  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                             >  1:877701/1‑76  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                             >  1:1831310/1‑76  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                             <  1:1334381/76‑1  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                             <  2:1719581/76‑1   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                            >  2:1004681/1‑76   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                            <  1:142453/76‑1   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                            >  2:1803936/1‑76   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                            >  1:359286/1‑76   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                            <  2:84847/76‑1   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCACTCTTCAGATGGCTCAANCCTTAAGTTAGCGCTTATGGG                                                                            >  2:318430/1‑76     GAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCC                                                                          >  2:581889/1‑76      AGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCG                                                                         <  2:1226404/76‑1      AGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCG                                                                         <  2:898948/76‑1      AGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCG                                                                         <  1:1745257/76‑1        GCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCA                                                                       >  2:410800/1‑76             GTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCC                                                                  <  2:102433/76‑1             GTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCC                                                                  >  2:605822/1‑76             GTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCC                                                                  >  2:448289/1‑76             GTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCC                                                                  >  2:605801/1‑76                 TAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGT                                                              >  2:1227706/1‑76                     GAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTG                                                          >  2:340333/1‑76                     GAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTG                                                          >  1:531785/1‑76                      AGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGC                                                         >  1:1064306/1‑76                      AGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGC                                                         >  2:1115450/1‑76                      AGGCACCGAGGCGTCCCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGC                                                         >  1:1443507/1‑76                        GCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTA                                                       <  1:663791/76‑1                         CACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAA                                                      >  1:1522769/1‑76                           CCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATG                                                    >  1:480412/1‑76                           CCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATG                                                    >  1:480398/1‑76                            CGAGGCGTCGCATTCTTCAGATGGTTTAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGC                                                   >  2:937108/1‑76                            CGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGC                                                   >  1:1666495/1‑76                             GAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCG                                                  <  1:863448/76‑1                              AGGCGTCGCATTCTTCAGATGGTTCAACCGTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGG                                                 <  2:333029/76‑1                                GCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGAT                                               <  2:1590880/76‑1                                GCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGAT                                               <  1:1909332/76‑1                                GCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGAT                                               <  1:842335/76‑1                                  GTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGC                                             >  2:163651/1‑76                                  GTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGC                                             <  1:882348/76‑1                                    CGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGA                                           <  1:285167/76‑1                                    CGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGA                                           >  1:688520/1‑76                                       atgCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCT                                        <  2:1763908/73‑1                                       ATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCT                                        <  1:598177/76‑1                                       ATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCT                                        >  1:1198801/1‑76                                       ATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCT                                        >  2:192191/1‑76                                        TTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTT                                       <  2:1383649/76‑1                                          CTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCA                                     <  2:1448561/76‑1                                          CTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCA                                     >  2:240517/1‑76                                           TTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAA                                    <  2:987538/76‑1                                             CAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCTAGG                                  >  2:1240937/1‑76                                               GATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTT                                <  1:1876649/76‑1                                               GATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTT                                <  1:1275027/76‑1                                               GATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTT                                <  2:172693/76‑1                                                ATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTC                               <  1:1682395/76‑1                                                  GGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAG                             >  1:1241063/1‑76                                                   GTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGT                            >  1:1660673/1‑76                                                   GTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGT                            >  1:1061401/1‑76                                                   GTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGT                            >  2:923362/1‑76                                                     TCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTAT                          <  2:1864548/76‑1                                                     TCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTAT                          <  1:1227706/76‑1                                                      CAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATC                         <  1:207129/76‑1                                                       AACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCA                        <  1:760417/76‑1                                                        ACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAA                       >  1:1268333/1‑76                                                          CCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATA                     >  2:467097/1‑76                                                          CCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATA                     <  1:439471/76‑1                                                           CTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAA                    <  1:1692044/76‑1                                                            TTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAAC                   <  1:204896/76‑1                                                            TTAAGTGAGCACTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAAC                   <  2:150496/76‑1                                                             TAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACC                  >  2:1560239/1‑76                                                             TAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACC                  >  1:1803644/1‑76                                                              AAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTCGTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCT                 <  2:1741044/76‑1                                                               AGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTA                <  1:294961/76‑1                                                               AGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTA                <  1:1220149/76‑1                                                                GTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTAC               <  1:1905627/76‑1                                                                 TTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACC              >  1:422059/1‑76                                                                  TAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCC             >  2:1589933/1‑76                                                                   AGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCA            >  2:970473/1‑76                                                                    GCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAG           <  2:1808674/76‑1                                                                    GCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAG           <  1:133182/76‑1                                                                        TTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTC       >  2:682582/1‑76                                                                        TTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTC       >  1:1042322/1‑76                                                                        TTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTC       >  2:52693/1‑76                                                                        TTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTC       >  1:1582554/1‑76                                                                        TTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTC       <  2:1783414/76‑1                                                                        TTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTC       >  2:1610805/1‑76                                                                        TTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTC       <  1:395683/76‑1                                                                         TATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCA      >  1:1593855/1‑76                                                                         TATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCA      >  1:1089784/1‑76                                                                           TGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGG    >  2:1139284/1‑76                                                                             GGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGA  <  2:740112/76‑1                                                                             GGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGA  <  2:1014524/76‑1                                                                                                                                                          ACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2774119‑2774196 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGA  >  REL606/4524522‑4524595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.