Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	1,670,487	Δ9 bp	coding (964‑972/1374 nt)	uidB ←	glucuronide transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	1670487	1670495	9	55 [0]	[0] 55	uidB	glucuronide transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 1670486	0 (0.000)	48 (0.860)	37/136	0.4	100%	coding (973/1374 nt)	uidB	glucuronide transporter
	?	REL606	1670496 =	0 (0.000)					coding (963/1374 nt)	uidB	glucuronide transporter

ATTCTACGGTATCAGCTTCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCC  >  REL606/1670411‑1670561                                                                            |                                                                            aTTCTACGGTATCAGCTTCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGC                                                                             >  2:1160372‑M1/1‑76 (MQ=255)    cTACGGTATCAGCTTCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcac                                                                          <  2:1082027‑M1/76‑4 (MQ=255)    cTACGGTATCAGCTTCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcac                                                                          <  2:1082036‑M1/76‑4 (MQ=255)     tACGGTATCAGCTTCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcacc                                                                         <  2:1497234‑M1/76‑5 (MQ=255)         gTATCAGCTTCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggca                                                                     >  2:1330775‑M1/1‑68 (MQ=255)         gTATCAGCTTCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggca                                                                     >  1:1909761‑M1/1‑68 (MQ=255)              aGCTTCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgac                                                                <  1:1923581‑M1/76‑14 (MQ=255)               gCTTCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgacc                                                               <  2:1150849‑M1/76‑15 (MQ=255)                cTTCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgacca                                                              <  1:1416788‑M1/76‑16 (MQ=255)                  tCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccaga                                                            <  1:707185‑M1/76‑18 (MQ=255)                  tCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccaga                                                            >  2:1343436‑M1/1‑59 (MQ=255)                    cAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacg                                                          <  1:1857426‑M1/76‑20 (MQ=255)                     aGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacgg                                                         <  2:1470271‑M1/76‑21 (MQ=255)                      gcgcCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacgga                                                        <  1:1932157‑M1/76‑22 (MQ=255)                             caTCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccag                                                 <  1:503325‑M1/76‑29 (MQ=255)                                cACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaag                                              >  1:923547‑M1/1‑45 (MQ=255)                                cACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaag                                              <  1:549421‑M1/76‑32 (MQ=255)                                  cGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaa                                            <  1:1747605‑M1/76‑34 (MQ=255)                                  cGGTAATGGTAACGCCCTGACCAATGGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaa                                            <  1:320603‑M1/76‑34 (MQ=255)                                   ggTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaac                                           <  1:1595700‑M1/76‑35 (MQ=255)                                       aTGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagca                                       <  1:141535‑M1/76‑39 (MQ=255)                                        tGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcacgggcagtgaccagacggaaacccagaagaacagcag                                      <  2:55786‑M1/76‑40 (MQ=255)                                        tGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcag                                      <  1:216045‑M1/76‑40 (MQ=255)                                          gTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagat                                    >  1:222802‑M1/1‑35 (MQ=255)                                          gTAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagat                                    <  1:926844‑M1/76‑42 (MQ=255)                                          gTAACGCCCTGACCAAGTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagat                                    <  1:1839045‑M1/76‑42 (MQ=255)                                           tAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagata                                   <  1:106709‑M1/76‑43 (MQ=255)                                           tAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagata                                   <  1:1937560‑M1/76‑43 (MQ=255)                                           tAACGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagata                                   <  1:106691‑M1/76‑43 (MQ=255)                                            agcgcCCTGACCAATTGAACCGATGGCCAACGCcaccggcagtgaccaggcggaaacccagaagaacagcagataa                                  <  2:1421699‑M1/74‑44 (MQ=255)                                              cGCCCTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataacc                                <  1:1788752‑M1/76‑46 (MQ=255)                                              cGCCCTGACCAATTGAAGCGATGGCCAACGCcaccgacagtgaccagacggaaacccagaagaacagcagataacc                                <  2:1458789‑M1/76‑46 (MQ=255)                                                 ccTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgca                             >  2:1922724‑M1/1‑28 (MQ=255)                                                 ccTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgca                             <  1:122293‑M1/76‑49 (MQ=255)                                                  cTGACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcag                            >  1:1909040‑M1/1‑27 (MQ=255)                                                    gACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggt                          <  2:1472391‑M1/76‑52 (MQ=255)                                                    gACCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggt                          <  1:1774864‑M1/76‑52 (MQ=255)                                                     aCCAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggtt                         <  1:606833‑M1/76‑53 (MQ=255)                                                       cAATTGAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggttcc                       <  2:1565881‑M1/76‑55 (MQ=255)                                                            gAAGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggttcccagca                  >  2:694381‑M1/1‑17 (MQ=255)                                                             aaGCGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggttcccagcaa                 >  2:1081463‑M1/1‑16 (MQ=255)                                                                cGATGGCCAACGCcaccggcagtgaccagacgtaaacccagaagaacagcagataaccgcaggttcccagcaaagc              <  2:192865‑M1/76‑64 (MQ=255)                                                                cGATGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggttcccagcaaagc              >  1:269354‑M1/1‑13 (MQ=255)                                                                  aTGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggttcccagcaaagcgc            >  1:531451‑M1/1‑11 (MQ=255)                                                                   tGGCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggttcccagcaaagcgcc           >  2:284515‑M1/1‑10 (MQ=255)                                                                    ggCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggttcccagcaaagcgcca          <  1:778629‑M1/76‑68 (MQ=255)                                                                    ggCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggttcccagcaaagcgcca          >  2:374255‑M1/1‑9 (MQ=255)                                                                    ggCCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggatcccagcaaagcgcca          >  2:1282328‑M1/1‑9 (MQ=255)                                                                    cgcCAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggttcccagcaaagcgcca          <  2:1312143‑M1/75‑68 (MQ=255)                                                                      ccAACGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggttcccagcaaagcgccaat        >  2:20875‑M1/1‑7 (MQ=255)                                                                        aaCGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggttcccagcaaagcgccaatca      <  2:530190‑M1/76‑72 (MQ=255)                                                                          cGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggttcccagcaaagcgccaatcagg    <  2:1349159‑M1/76‑74 (MQ=255)                                                                          cGCcaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggttcccagcaaagcgccaatcagg    <  2:1827964‑M1/76‑74 (MQ=255)                                                                           gCccccggcagtgtccagacggaaacccagaagaacagcagataaccgcaggttcccagcaaagcgccaatcagga   <  1:62867‑M1/76‑75 (MQ=255)                                                                            ccaccggcagtgaccagacggaaacccagaagaacagcagataaccgcaggttcccagcaaagcgccaatcaggaa  <  1:1703114‑M1/76‑76 (MQ=255)                                                                            |                                                                            ATTCTACGGTATCAGCTTCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCC  >  REL606/1670411‑1670561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑