| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,484,105 | 0 | A | G | 36.1% | 59.2 / 16.2 | 36 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (2/21); new base G (13/0); total (15/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.54e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAA > REL606/1484033‑1484179 | aCTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAttt < 2:963305/76‑1 (MQ=255) tgctgcTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTta < 2:1105629/76‑1 (MQ=255) ctgctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGGCACTGTTATGGCAGGGGttttttt > 2:249115/1‑73 (MQ=255) ctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACtt < 2:1338270/76‑1 (MQ=255) ttGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACttt < 2:1109205/76‑1 (MQ=255) tGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCtttt > 2:682457/1‑76 (MQ=255) tGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTACtttt > 1:1095663/1‑76 (MQ=255) cccACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACttttttc > 2:456119/1‑73 (MQ=255) cccACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGAc < 2:939209/76‑1 (MQ=255) cTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGAtt > 1:1817608/1‑76 (MQ=255) cTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAtt < 1:182011/76‑1 (MQ=255) tAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTGCGAttt > 2:1923161/1‑76 (MQ=255) tAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTACGAttt > 2:213859/1‑76 (MQ=255) tAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTATTTTTTCGAttt > 1:316540/1‑76 (MQ=255) aTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTATTTTTGAGGAatttc > 1:557353/1‑74 (MQ=255) tgtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTAc > 1:1545294/1‑76 (MQ=255) ggTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCAccct > 2:1143631/1‑74 (MQ=255) tttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGt < 1:1817511/76‑1 (MQ=255) tttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGt < 1:1512254/76‑1 (MQ=255) ttGGCATTTTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTATTTTTATGATTTTACCATGTc > 1:52959/1‑76 (MQ=255) tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTACCATTTCg > 1:576566/1‑76 (MQ=255) tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCACCATGTCg > 1:553998/1‑76 (MQ=255) tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCg < 2:755455/76‑1 (MQ=255) tGGCACTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTACGATTTTACCATGTCg > 1:1306315/1‑76 (MQ=255) gCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGt < 1:244620/76‑1 (MQ=255) cATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTa < 1:1121388/76‑1 (MQ=255) gCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGAc < 1:294473/76‑1 (MQ=255) cGCTGCTGACACTGTTGTGGCAGGGGTTTTTTACTTTTGACGATTTCACCATGTCGGGAACGCCGGAACTGACGCt > 1:1549401/1‑76 (MQ=255) gctgctGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTg < 2:1727011/76‑1 (MQ=255) gctgctGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTg < 2:538162/76‑1 (MQ=255) ctgctgACACTGTTATGGCAGGGGTTTTTTACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTgg > 1:1148605/1‑76 (MQ=255) gctgACACTGTTATGGCAGGGGTTTTTTACTTTTGACGATTTCACCATGTCGGTAACGCCGGAAATGACGCTGGCg > 1:874968/1‑76 (MQ=255) ctgACACTGTTATGGCAGGGGTTTTATACTTTTTACGATTTCACCATGTCGGTAACGCCGGAACTGACGCCGGGGa > 1:1820269/1‑76 (MQ=255) tgACACTGTTATGGCAGGGGTTTTTTACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGaa > 2:297685/1‑76 (MQ=255) gACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAat < 1:554925/76‑1 (MQ=255) cacTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAatat < 2:1860757/76‑1 (MQ=255) cacTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAatat < 2:948764/76‑1 (MQ=255) gTTATGGCAGGGGTTTTTTACTTTTGACGATTTCACCATGTCGGGAAACCCCGAACTGACGCTGGCGGATATccct > 2:444839/1‑74 (MQ=255) ttATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTg < 1:1890150/76‑1 (MQ=255) tATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTgc < 1:1808615/76‑1 (MQ=255) aTGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTgcg < 1:1267334/76‑1 (MQ=255) aTGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTgcg > 1:1935864/1‑76 (MQ=255) cAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGt > 2:1939911/1‑76 (MQ=255) gATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTaa < 2:300841/76‑1 (MQ=255) | ACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAA > REL606/1484033‑1484179 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |