Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,339,247	A→G	D29G (GAC→GGC)	fis →	DNA‑binding protein Fis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,339,247	0	A	G	98.7%	325.7 / ‑3.8	79	D29G (GAC→GGC)	fis	DNA‑binding protein Fis
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (41/37);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01

CGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGC  >  REL606/3339174‑3339318                                                                          |                                                                        cgcgTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCt                                                                       >  2:1147301/1‑76 (MQ=255)  gcgTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTc                                                                      <  1:1503988/76‑1 (MQ=255)   cgTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCg                                                                     >  1:455339/1‑76 (MQ=255)      aaaTTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGtt                                                                  >  2:541194/1‑76 (MQ=255)      aaaTTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGtt                                                                  <  2:192181/76‑1 (MQ=255)      aaaTTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGtt                                                                  <  2:327918/76‑1 (MQ=255)       aaTTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCANAAACCCCTGCGTGGCTCGGTTa                                                                 <  2:256746/76‑1 (MQ=255)       aaTTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTa                                                                 >  1:724547/1‑76 (MQ=255)          tCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAACCCCCTGCGTGGCTCGGTTAAAc                                                              <  1:395271/76‑1 (MQ=255)          tCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTaaaa                                                              <  1:961893/76‑2 (MQ=255)           cTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACa                                                             <  1:441838/76‑1 (MQ=255)            tGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAg                                                            >  2:64529/1‑76 (MQ=255)             gACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAgg                                                           >  2:958842/1‑76 (MQ=255)             gACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAgg                                                           <  2:1241287/76‑1 (MQ=255)             gACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAgg                                                           >  1:1644256/1‑76 (MQ=255)              aCGTACTGACTGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGc                                                          <  1:826372/76‑1 (MQ=255)              aCGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGc                                                          <  1:189541/76‑1 (MQ=255)              aCGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGc                                                          >  2:1797036/1‑76 (MQ=255)               cGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCa                                                         <  1:1080015/76‑1 (MQ=255)                 tACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACt                                                       >  1:1283623/1‑76 (MQ=255)                 tACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACt                                                       >  1:498138/1‑76 (MQ=255)                 tACTGACCGTTTCTACCGTTAACTATCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACt                                                       >  2:1818141/1‑76 (MQ=255)                  aCTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTg                                                      >  2:1239595/1‑76 (MQ=255)                    tGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTgaa                                                    >  2:1865156/1‑76 (MQ=255)                    tGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTgaa                                                    >  2:908667/1‑76 (MQ=255)                     gACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTgaag                                                   <  1:1845347/76‑1 (MQ=255)                     gACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTgaag                                                   >  2:475256/1‑76 (MQ=255)                     gACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTgaag                                                   <  1:1601029/76‑1 (MQ=255)                      aCCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTgaaga                                                  <  2:1208998/76‑1 (MQ=255)                       ccGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTgaagaa                                                 >  1:315783/1‑76 (MQ=255)                          tttCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTa                                              <  2:1272200/76‑1 (MQ=255)                          tttCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTa                                              >  1:1257493/1‑76 (MQ=255)                            tCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTAtt                                            <  2:1241019/76‑1 (MQ=255)                            tCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTAtt                                            <  2:1241026/76‑1 (MQ=255)                             cTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTAttt                                           >  2:203768/1‑76 (MQ=255)                              tACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTAtttt                                          <  1:615468/76‑1 (MQ=255)                              tACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTAtttt                                          <  1:1750105/76‑1 (MQ=255)                              tACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTAtttt                                          >  1:1603591/1‑76 (MQ=255)                               aCCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTg                                         <  2:858877/76‑1 (MQ=255)                                ccGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGc                                        <  1:752979/76‑1 (MQ=255)                                ccGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGc                                        <  1:795757/76‑1 (MQ=255)                                 cGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCt                                       <  1:871580/76‑1 (MQ=255)                                   ttAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCa                                     <  1:684187/76‑1 (MQ=255)                                     aaCTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAc                                   >  1:1032747/1‑76 (MQ=255)                                      aCTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACt                                  <  1:743665/76‑1 (MQ=255)                                      aCTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACt                                  <  1:357872/76‑1 (MQ=255)                                       ctctCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTg                                 >  2:988785/1‑76 (MQ=255)                                       ctctCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTg                                 >  1:647264/1‑76 (MQ=255)                                         ctcAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGaa                               <  2:991004/76‑1 (MQ=255)                                         ctcAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGaa                               >  1:1639946/1‑76 (MQ=255)                                         ctcAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGaa                               >  1:452267/1‑76 (MQ=255)                                         ctcAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGaa                               >  2:1544979/1‑76 (MQ=255)                                         ctcAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGaa                               <  1:502653/76‑1 (MQ=255)                                          tcAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAAt                              >  1:1623484/1‑76 (MQ=255)                                           cAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATg                             >  2:1143737/1‑76 (MQ=255)                                           cAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATg                             <  2:1827563/76‑1 (MQ=255)                                           cAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATg                             <  2:796963/76‑1 (MQ=255)                                            aGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATgg                            >  1:657792/1‑76 (MQ=255)                                              gATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTc                          >  1:565960/1‑76 (MQ=255)                                                tCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAg                        <  2:90541/76‑1 (MQ=255)                                                tCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAg                        <  2:736366/76‑1 (MQ=255)                                                tCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAg                        >  1:204700/1‑76 (MQ=255)                                                tCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAg                        <  1:143039/76‑1 (MQ=255)                                                 cAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAgg                       <  1:1144639/76‑1 (MQ=255)                                                    gTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGAtg                    <  1:1916623/76‑1 (MQ=255)                                                    gTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGAtg                    >  1:176923/1‑76 (MQ=255)                                                    gTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGAtg                    >  1:488167/1‑76 (MQ=255)                                                     tAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGAtgt                   >  2:785678/1‑76 (MQ=255)                                                      aaCCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGAtgtg                  >  2:1062360/1‑76 (MQ=255)                                                        cccAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGaa                <  1:1484931/76‑1 (MQ=255)                                                           aaaaaCCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGa             >  1:1664397/1‑76 (MQ=255)                                                           aaaaaCCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGa             <  2:897080/76‑1 (MQ=255)                                                           aaaaaCCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGa             >  1:508397/1‑76 (MQ=255)                                                            aaaaCCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGAc            >  2:1431043/1‑76 (MQ=255)                                                            aaaaCCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGAc            >  2:1421237/1‑76 (MQ=255)                                                             aaaCCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGAcc           >  1:315922/1‑76 (MQ=255)                                                                ccccTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACctct        >  2:558659/1‑76 (MQ=255)                                                                      cGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGc  >  1:148867/1‑76 (MQ=255)                                                                      cGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGc  >  2:1678379/1‑76 (MQ=255)                                                                          |                                                                        CGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGC  >  REL606/3339174‑3339318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑