Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,438,531	T→G	D110A (GAC→GCC)	fbp ←	fructose‑1,6‑bisphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,438,531	0	T	G	100.0%	224.9 / NA	53	D110A (GAC→GCC)	fbp	fructose‑1,6‑bisphosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/35);  total (18/35)

AACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGC  >  REL606/4438458‑4438601                                                                          |                                                                       aaCGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGcc                                                                      >  1:1038957/1‑76 (MQ=255)    gcggcgGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATc                                                                   <  1:96894/76‑1 (MQ=255)    gcggcgGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATc                                                                   <  2:164958/76‑1 (MQ=255)     cggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCa                                                                  <  2:977422/76‑1 (MQ=255)     cggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCa                                                                  <  2:155539/76‑1 (MQ=255)     cggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCa                                                                  <  1:360341/76‑1 (MQ=255)     cggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCa                                                                  >  2:1836326/1‑76 (MQ=255)       gcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGc                                                                <  2:1734960/76‑1 (MQ=255)          gTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacc                                                             >  2:506638/1‑76 (MQ=255)            aGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcaccac                                                           >  1:1834216/1‑76 (MQ=255)            aGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcaccac                                                           >  1:280558/1‑76 (MQ=255)            aGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcaccac                                                           <  2:1682220/76‑1 (MQ=255)               tGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTa                                                        >  1:248325/1‑76 (MQ=255)                ggAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTAt                                                       >  1:675016/1‑76 (MQ=255)                  agaAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTAttt                                                     <  2:766238/76‑1 (MQ=255)                   gaAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTAtttt                                                    <  2:1911333/76‑1 (MQ=255)                   gaAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTAtttt                                                    <  2:515083/76‑1 (MQ=255)                      aaTGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCg                                                 >  1:382405/1‑76 (MQ=255)                      aaTGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCg                                                 >  2:151606/1‑76 (MQ=255)                      aaTGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCg                                                 >  2:76062/1‑76 (MQ=255)                        tGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCgtg                                               <  2:1775195/76‑1 (MQ=255)                         ggTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCgtgt                                              >  1:1440825/1‑76 (MQ=255)                          gTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGtt                                             <  2:649896/76‑1 (MQ=255)                            aCCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTca                                           >  1:1377930/1‑76 (MQ=255)                             ccGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcac                                          <  2:1610093/76‑1 (MQ=255)                              cGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcaca                                         <  2:1890146/76‑1 (MQ=255)                              cGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcaca                                         <  2:1430459/76‑1 (MQ=255)                              cGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcaca                                         <  1:666975/76‑1 (MQ=255)                                   gagaCGTTAACATAGATGGTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCtt                                    <  2:356282/76‑1 (MQ=255)                                     gaCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCa                                  <  1:1322107/76‑1 (MQ=255)                                     gaCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCa                                  <  1:1836326/76‑1 (MQ=255)                                       cGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaaa                                <  1:1335833/76‑1 (MQ=255)                                       cGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaaa                                <  2:443467/76‑1 (MQ=255)                                        gTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAg                               <  1:1405276/76‑1 (MQ=255)                                        gTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAg                               <  1:1405280/76‑1 (MQ=255)                                         ttAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAga                              <  2:92917/76‑1 (MQ=255)                                         ttAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAga                              <  2:1811054/76‑1 (MQ=255)                                           aaCATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacg                            >  2:1451557/1‑76 (MQ=255)                                                  aTGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAtct                     <  1:1284225/76‑1 (MQ=255)                                                  aTGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAtct                     >  1:584941/1‑76 (MQ=255)                                                        gACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtctt               <  2:329912/76‑1 (MQ=255)                                                         aCGATCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttc              >  1:1869003/1‑76 (MQ=255)                                                         aCGAGCCATCCAGGGGGGCCATCAGCACCGCGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttc              <  2:15535/76‑1 (MQ=255)                                                           gAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttctt            >  1:175187/1‑76 (MQ=255)                                                           gAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttctt            <  2:1308307/76‑1 (MQ=255)                                                           gAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttctt            <  2:1459922/76‑1 (MQ=255)                                                            aGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttcttc           <  1:689353/76‑1 (MQ=255)                                                              ccATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATcttcttctt         >  1:266629/1‑76 (MQ=255)                                                                aTCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCa       <  1:901316/76‑1 (MQ=255)                                                                aTCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCa       <  2:358448/76‑1 (MQ=255)                                                                 tCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCag      <  2:1377226/76‑1 (MQ=255)                                                                 tCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCag      <  2:738999/76‑1 (MQ=255)                                                                 tCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCag      <  1:1562726/76‑1 (MQ=255)                                                                    aGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAgg   >  2:1299574/1‑76 (MQ=255)                                                                     ggggggCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGc  <  2:695460/76‑1 (MQ=255)                                                                     ggggggCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGc  <  2:1036122/76‑1 (MQ=255)                                                                          |                                                                       AACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGC  >  REL606/4438458‑4438601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑