BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,844,557	C→T	R233W (CGG→TGG)	argA →	N‑acetylglutamate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,844,557	0	C	T	100.0%	183.0 / NA	48	R233W (CGG→TGG)	argA	N‑acetylglutamate synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/20); total (28/20)

TTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAG  >  REL606/2844484‑2844629

ttGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTgg                                                                        <  1:42376/76‑1 (MQ=255)

 tGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTggg                                                                       >  2:740389/1‑76 (MQ=255)

     ctTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGa                                                                   >  2:991202/1‑76 (MQ=255)

       tCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAg                                                                 >  2:1777798/1‑76 (MQ=255)

       tCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAg                                                                 >  2:1202390/1‑76 (MQ=255)

       tCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAg                                                                 >  1:747986/1‑76 (MQ=255)

           aGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCa                                                             >  2:1450038/1‑76 (MQ=255)

           aGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCa                                                             >  2:1231667/1‑76 (MQ=255)

           aGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCa                                                             <  1:930973/76‑1 (MQ=255)

            gggCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAg                                                            <  2:991960/76‑1 (MQ=255)

            gggCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAg                                                            <  2:704479/76‑1 (MQ=255)

              gCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGa                                                          <  1:835615/76‑1 (MQ=255)

                gTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAag                                                        <  1:1879314/76‑1 (MQ=255)

                 tCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCACGCGTGGGTAGAAGCCCAGGAaga                                                       >  1:1732436/1‑76 (MQ=255)

                  cACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAagag                                                      <  2:948524/76‑1 (MQ=255)

                   aCTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAgaga                                                     >  2:1840665/1‑76 (MQ=255)

                   aCTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAgaga                                                     >  2:282984/1‑76 (MQ=255)

                   aCTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAgaga                                                     <  1:946633/76‑1 (MQ=255)

                   aCTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAgaga                                                     >  1:832479/1‑76 (MQ=255)

                   aCTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAgaga                                                     <  1:821427/76‑1 (MQ=255)

                   aCTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAgaga                                                     <  1:299019/76‑1 (MQ=255)

                   aCTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAgaga                                                     <  1:1824588/76‑1 (MQ=255)

                     tAATGACGACTGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGaaa                                                   <  1:98617/76‑1 (MQ=255)

                      aaTGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAg                                                  >  2:1281548/1‑76 (MQ=255)

                      aaTGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAg                                                  >  1:1731646/1‑76 (MQ=255)

                              cgGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTan                                          <  1:1916024/76‑2 (MQ=255)

                                gTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACaa                                        >  1:671210/1‑76 (MQ=255)

                                gTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACaa                                        >  2:510445/1‑76 (MQ=255)

                                gTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACaa                                        >  2:354810/1‑76 (MQ=255)

                                gTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTACAAGCCCAGGAAGAGAAAGGCGATTACaa                                        >  1:1287996/1‑76 (MQ=255)

                                  gATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACt                                      <  1:1751358/76‑1 (MQ=255)

                                   atatTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTc                                     >  2:248137/1‑76 (MQ=255)

                                   atatTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGCTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTc                                     >  1:1152193/1‑76 (MQ=255)

                                       tGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGt                                 <  2:1908739/76‑1 (MQ=255)

                                        gTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTa                                <  2:236994/76‑1 (MQ=255)

                                         tctcAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTAc                               >  1:1206645/1‑76 (MQ=255)

                                         tctcAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTAc                               <  2:1794693/76‑1 (MQ=255)

                                          ctcAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACg                              <  1:150958/76‑1 (MQ=255)

                                            cAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGt                            >  2:848539/1‑76 (MQ=255)

                                            cAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGt                            >  1:220157/1‑76 (MQ=255)

                                             aGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTg                           >  2:664937/1‑76 (MQ=255)

                                              gAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTgc                          >  2:374928/1‑76 (MQ=255)

                                                   tttCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCttt                     <  2:1080491/76‑1 (MQ=255)

                                                   tttCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCttt                     <  2:731130/76‑1 (MQ=255)

                                                      cccTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTg                  >  1:1556496/1‑76 (MQ=255)

                                                       ccTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGc                 <  1:595438/76‑1 (MQ=255)

                                                            cGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGgc            >  1:1472645/1‑76 (MQ=255)

                                                            cGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGgc            >  1:1711179/1‑76 (MQ=255)

                                                             gAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGgcg           >  1:943998/1‑76 (MQ=255)

                                                               agcgcaagcgtGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCa         >  1:1614943/1‑76 (MQ=255)

                                                               agcgcaagcgtGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCa         >  1:1313731/1‑76 (MQ=255)

                                                                    aagcgtGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGan    <  1:373562/76‑2 (MQ=255)

                                                                    aagcgtGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGaa    >  2:543968/1‑76 (MQ=255)

                                                                      gcgtGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAg  <  1:492872/76‑1 (MQ=255)

                                                                      gcgtGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAg  <  1:1777798/76‑1 (MQ=255)

TTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAG  >  REL606/2844484‑2844629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`