Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	473,924	A→C	V461G (GTC→GGC)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	473,924	0	A	C	100.0%	206.9 / NA	51	V461G (GTC→GGC)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/17);  total (34/17)

CAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGA  >  REL606/473851‑473996                                                                          |                                                                         cAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGccc                                                                        <  2:722598/76‑1 (MQ=255)        tttGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGct                                                                 >  1:575643/1‑76 (MQ=255)        tttGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGct                                                                 >  2:1625140/1‑76 (MQ=255)           tcacaTTCCTGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATCCTGCGCGGCCCCCGCGCtctc                                                              <  1:305338/75‑1 (MQ=255)           gCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCtctc                                                              <  1:1397861/76‑1 (MQ=255)           gCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCtctc                                                              <  2:575232/76‑1 (MQ=255)           gCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCtctc                                                              <  2:482926/76‑1 (MQ=255)             acaTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGc                                                            <  2:671019/76‑1 (MQ=255)             acaTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGc                                                            <  2:305422/76‑1 (MQ=255)              caTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCa                                                           <  2:1315232/76‑1 (MQ=255)              caTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCa                                                           <  2:494954/76‑1 (MQ=255)               aTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAg                                                          <  1:638080/76‑1 (MQ=255)               aTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAg                                                          >  2:831218/1‑76 (MQ=255)               aTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAg                                                          >  2:944506/1‑76 (MQ=255)                   cAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtca                                                      >  2:467881/1‑76 (MQ=255)                    aGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcat                                                     >  1:246632/1‑76 (MQ=255)                    aGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcat                                                     >  1:1271151/1‑76 (MQ=255)                      aTGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatca                                                   <  2:94888/76‑1 (MQ=255)                      aTGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatca                                                   >  1:68122/1‑76 (MQ=255)                       tGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCaa                                                  <  2:430884/76‑1 (MQ=255)                         cgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAaca                                                >  1:616285/1‑76 (MQ=255)                          gcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAacac                                               >  1:1032766/1‑76 (MQ=255)                          gcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAacac                                               >  1:15163/1‑76 (MQ=255)                            cAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACgg                                             <  1:355849/76‑1 (MQ=255)                            cAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACgg                                             >  2:28354/1‑76 (MQ=255)                               ttGCATAATTTCCTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGtt                                          >  2:1366007/1‑76 (MQ=255)                                tGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTc                                         >  1:1320784/1‑76 (MQ=255)                                    tAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGCTCGTgc                                     >  2:18152/1‑75 (MQ=255)                                        ttcttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtc                                 >  2:945943/1‑76 (MQ=255)                                             cgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAAt                            <  1:851841/76‑1 (MQ=255)                                             cgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAAt                            <  1:1520414/76‑1 (MQ=255)                                              gttcgCCGCATTGCCCGATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATc                           <  2:154417/76‑1 (MQ=255)                                              gttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATc                           <  1:39878/76‑1 (MQ=255)                                               ttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATca                          >  2:1512844/1‑76 (MQ=255)                                                tcgCCGCATTGCCCAATTCTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcac                         <  2:6368/76‑1 (MQ=255)                                                    cgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCaccacc                     >  2:1034654/1‑76 (MQ=255)                                                     gcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCAccacca                    >  1:924352/1‑76 (MQ=255)                                                     gcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCAccacca                    >  1:95581/1‑76 (MQ=255)                                                     gcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGGTCGTGACGCCTCAATCAccccca                    >  1:1116736/1‑76 (MQ=255)                                                      cATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAg                   >  1:539372/1‑76 (MQ=255)                                                         tGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCgg                >  1:1634673/1‑76 (MQ=255)                                                           cccAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGaa              >  2:1164310/1‑76 (MQ=255)                                                             cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata            >  2:1356576/1‑76 (MQ=255)                                                             cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata            >  2:639848/1‑76 (MQ=255)                                                             cAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata            >  2:1116278/1‑76 (MQ=255)                                                               aTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATc          >  1:1408922/1‑76 (MQ=255)                                                                tACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCa         >  2:639404/1‑76 (MQ=255)                                                                tACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCa         >  2:1666284/1‑76 (MQ=255)                                                                tACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTACGTGACGGCTCAACCACCCCCACCCGAATATCa         >  2:45643/1‑76 (MQ=255)                                                                  cTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGATGACTAAATCACCACCAGCGGAATATCaga       >  2:1413781/1‑76 (MQ=255)                                                                  cTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCaga       <  1:73796/76‑1 (MQ=255)                                                                    gcgcGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAgg     >  2:1690931/1‑76 (MQ=255)                                                                       cgGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGa  >  1:1659023/1‑76 (MQ=255)                                                                          |                                                                         CAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGA  >  REL606/473851‑473996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑