Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,232,667	T→G	I236L (ATC→CTC)	yraQ ←	predicted permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,232,667	0	T	G	100.0%	199.8 / NA	49	I236L (ATC→CTC)	yraQ	predicted permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/22);  total (27/22)

TGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAAACCGCTCTGTG  >  REL606/3232595‑3232738                                                                         |                                                                        tGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTa                                                                      <  2:593490/76‑1 (MQ=255) tGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTa                                                                      <  2:916379/76‑1 (MQ=255)  gTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTaa                                                                     <  1:371899/76‑1 (MQ=255)    tATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAAc                                                                   >  1:349361/1‑76 (MQ=255)    tATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAAc                                                                   >  1:695339/1‑76 (MQ=255)     aTCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAAcc                                                                  <  2:352663/76‑1 (MQ=255)      tCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCg                                                                 <  1:1454693/76‑1 (MQ=255)         aCAGCACCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGCAAACCGGGa                                                              >  2:813426/1‑76 (MQ=255)           aGCACCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATc                                                            >  1:1521879/1‑76 (MQ=255)           aGCACCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATc                                                            >  1:1430716/1‑76 (MQ=255)           aGCACCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGCAAACCGGGATc                                                            >  1:1093571/1‑76 (MQ=255)            gccccATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCg                                                           <  1:32553/73‑1 (MQ=255)            gCACCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCg                                                           >  1:1403014/1‑76 (MQ=255)              aCCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTa                                                         >  2:124715/1‑76 (MQ=255)               ccATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTAc                                                        >  2:1577330/1‑76 (MQ=255)               ccATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTAc                                                        >  2:1184159/1‑76 (MQ=255)               ccATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTAc                                                        >  2:1114757/1‑76 (MQ=255)                cATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGGAAACCGGGATCGTACt                                                       >  2:1104619/1‑76 (MQ=255)                 aTCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTc                                                      <  2:734521/76‑1 (MQ=255)                  tCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTcc                                                     >  1:422021/1‑76 (MQ=255)                     gCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGa                                                  >  2:1491046/1‑76 (MQ=255)                     gCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGa                                                  <  1:1716440/76‑1 (MQ=255)                      cATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGaa                                                 >  1:757007/1‑76 (MQ=255)                       aTGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGaaa                                                >  1:678959/1‑76 (MQ=255)                       aTGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGaaa                                                >  1:1352504/1‑76 (MQ=255)                       aTGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGaaa                                                >  1:1234147/1‑76 (MQ=255)                       aTGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGaaa                                                <  1:1178619/76‑1 (MQ=255)                         gggggAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAg                                              >  1:295532/1‑76 (MQ=255)                          ggggAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGc                                             >  2:1638095/1‑76 (MQ=255)                           gggAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCg                                            <  1:567931/76‑1 (MQ=255)                              aaTAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTcc                                         <  2:400031/76‑1 (MQ=255)                               aTAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCa                                        <  2:1098924/76‑1 (MQ=255)                                tAACCAGACTCGTGCGGCACCCACCCCCAGTACTGCCAGGAGGTAAACCGGGATCGTACTCCAGACCAGCGTctct                                       >  2:1270661/1‑73 (MQ=255)                                tAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCAt                                       <  1:1460280/76‑1 (MQ=255)                                 aaCCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATa                                      <  1:1147299/76‑1 (MQ=255)                                  aCCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAg                                     <  1:124715/76‑1 (MQ=255)                                   ccAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAgc                                    >  2:1034898/1‑76 (MQ=255)                                   ccAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAgc                                    <  2:1310241/76‑1 (MQ=255)                                         gcgTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTg                              <  2:1553250/76‑1 (MQ=255)                                           gTGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGcc                            >  1:87840/1‑76 (MQ=255)                                            tGCGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGccc                           >  1:68636/1‑76 (MQ=255)                                              cGGCACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCa                         >  1:369360/1‑76 (MQ=255)                                                 cACCCAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCg                      >  1:297858/1‑76 (MQ=255)                                                    ccAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCt                   >  1:830533/1‑76 (MQ=255)                                                    ccAACACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCt                   <  1:1268108/76‑1 (MQ=255)                                                      aaCACCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTaa                 <  2:1294170/76‑1 (MQ=255)                                                        cacCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTaaaa               <  1:649977/76‑1 (MQ=255)                                                         acCAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTaaaaa              >  1:21517/1‑76 (MQ=255)                                                          ccAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTaaaaaa             <  1:1479863/76‑1 (MQ=255)                                                          ccAGTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTaaaaaa             <  1:1479850/76‑1 (MQ=255)                                                             gTACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAAAcc          <  1:517224/76‑1 (MQ=255)                                                              tACTGCAAGGAGGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAAACCg         >  2:1409142/1‑76 (MQ=255)                                                                     aggaggTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAAACCGCTCtgtg  >  2:140939/1‑76 (MQ=255)                                                                     aggaggTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAAACCGCTCtgtg  <  2:226080/76‑1 (MQ=255)                                                                     aggaggTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAAACCGCTCtgtg  >  2:992462/1‑76 (MQ=255)                                                                         |                                                                        TGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGTGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAAACCGCTCTGTG  >  REL606/3232595‑3232738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑