Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,273,299	A→T	intergenic (+227/‑115)	nrfG → / → gltP	heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/glutamate/aspartate:proton symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,273,299	0	A	T	100.0%	267.9 / NA	66	intergenic (+227/‑115)	nrfG/gltP	heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/glutamate/aspartate:proton symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (41/25);  total (41/25)

GTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCG  >  REL606/4273227‑4273371                                                                         |                                                                         gTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCa                                                                       >  2:188848/1‑76 (MQ=255) gTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCa                                                                       <  1:173723/76‑1 (MQ=255) gTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCa                                                                       <  2:1548512/76‑1 (MQ=255) gTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCa                                                                       <  2:170179/76‑1 (MQ=255)  ttattaTGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCAt                                                                      >  2:924071/1‑76 (MQ=255)  ttattaTGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCAt                                                                      <  2:1660684/76‑1 (MQ=255)  ttattaTGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCAt                                                                      <  2:1164790/76‑1 (MQ=255)  ttattaTGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCAt                                                                      <  1:293305/76‑1 (MQ=255)   tattatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATa                                                                     <  2:574714/76‑1 (MQ=255)   tattatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATa                                                                     >  1:1501267/1‑76 (MQ=255)   tattatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATa                                                                     >  1:154649/1‑76 (MQ=255)     ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg                                                                   <  1:8911/76‑1 (MQ=255)       atGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCg                                                                 >  1:1032777/1‑76 (MQ=255)           aTTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaa                                                             >  2:1454929/1‑76 (MQ=255)             tAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaa                                                           <  1:302118/76‑1 (MQ=255)              aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa                                                          <  1:381927/76‑1 (MQ=255)               aTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaaa                                                         >  1:665367/1‑76 (MQ=255)                   aGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCt                                                     >  2:1190613/1‑76 (MQ=255)                   aGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCt                                                     >  1:1425348/1‑76 (MQ=255)                     cGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTAc                                                   <  1:410230/76‑1 (MQ=255)                     cGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTAc                                                   <  1:523560/76‑1 (MQ=255)                      gAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACa                                                  >  1:170369/1‑76 (MQ=255)                      gAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACa                                                  <  1:1262355/76‑1 (MQ=255)                      gAAAAGCTCCGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACa                                                  <  1:1262367/76‑1 (MQ=255)                        aaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATATTGTTCATTAGCGTTAAAAAAATCTACaaa                                                >  2:293937/1‑76 (MQ=255)                        aaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACaaa                                                >  2:1232465/1‑76 (MQ=255)                        aaaGCTCTGTTGTTAAAGGGCTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTACAAAAATCTACaaa                                                >  2:1176204/1‑76 (MQ=255)                         aaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAAc                                               >  2:736991/1‑76 (MQ=255)                         aaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAAc                                               >  1:714468/1‑76 (MQ=255)                           gCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCa                                             <  1:901165/76‑1 (MQ=255)                           gCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCa                                             <  2:1718528/76‑1 (MQ=255)                           gCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCa                                             >  1:1124052/1‑76 (MQ=255)                            ctctGTTGTTAAAGGGTTGCGCAACATACCGCGCAACTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCaa                                            >  2:1219492/1‑76 (MQ=255)                            ctctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCaa                                            <  1:450809/76‑1 (MQ=255)                            ctctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCaa                                            >  2:489964/1‑76 (MQ=255)                             tctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAAc                                           >  1:1641537/1‑76 (MQ=255)                               tgttgtTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGc                                         >  1:1239008/1‑76 (MQ=255)                                 ttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCaa                                       >  1:1063380/1‑76 (MQ=255)                                 ttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCaa                                       <  1:407321/76‑1 (MQ=255)                                    ttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAacac                                    >  1:821880/1‑76 (MQ=255)                                    ttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAacac                                    <  2:523109/76‑1 (MQ=255)                                     tAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAAcaca                                   >  2:1381933/1‑76 (MQ=255)                                      aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACaa                                  >  2:1529198/1‑76 (MQ=255)                                       aaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAAt                                 >  2:658029/1‑76 (MQ=255)                                        aGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAAtt                                >  2:759440/1‑76 (MQ=255)                                          ggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCa                              >  1:1605512/1‑76 (MQ=255)                                          ggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCa                              <  2:399927/76‑1 (MQ=255)                                             tGCGCAACATACCGCGCAAATGATATTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGc                           >  1:637679/1‑76 (MQ=255)                                             tGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGc                           >  1:98260/1‑76 (MQ=255)                                             tGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGc                           >  1:1058422/1‑76 (MQ=255)                                                gcAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCt                        <  2:1493296/76‑1 (MQ=255)                                                gcAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCt                        >  1:260184/1‑76 (MQ=255)                                                gcAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCt                        >  1:1013785/1‑76 (MQ=255)                                                  aaCATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTgg                      >  2:968917/1‑76 (MQ=255)                                                  aaCATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTgg                      >  1:651966/1‑76 (MQ=255)                                                       aCCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCACGCCCTGGCAGTa                 >  1:942167/1‑76 (MQ=255)                                                         cgcgCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATg               <  1:1159662/76‑1 (MQ=255)                                                           cgcAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTc             >  1:471423/1‑76 (MQ=255)                                                               aaTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGt         >  2:1100576/1‑76 (MQ=255)                                                                aTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGtt        >  2:1533542/1‑76 (MQ=255)                                                                aTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGtt        <  1:185069/76‑1 (MQ=255)                                                                   aTACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc     <  2:258009/76‑1 (MQ=255)                                                                      cTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcgcg  >  2:1703870/1‑76 (MQ=255)                                                                      cTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcgcg  <  2:1606966/76‑1 (MQ=255)                                                                      cTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcgcg  >  1:1043764/1‑76 (MQ=255)                                                                      cTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcgcg  >  1:1031662/1‑76 (MQ=255)                                                                         |                                                                         GTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCG  >  REL606/4273227‑4273371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑