Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,347,125	C→T	intergenic (‑77/‑260)	aspA ← / → fxsA	aspartate ammonia‑lyase/inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,347,125	0	C	T	100.0%	246.7 / NA	60	intergenic (‑77/‑260)	aspA/fxsA	aspartate ammonia‑lyase/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/29);  total (31/29)

AACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACCGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTACCGTAAT  >  REL606/4347052‑4347191                                                                          |                                                                   aaCCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTcgactga                                                                  >  1:991613/1‑76 (MQ=255)    cttcttTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACtgacga                                                               >  2:799378/1‑76 (MQ=255)      tcttTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGAtt                                                             <  2:137611/76‑1 (MQ=255)        tttttCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTAt                                                           <  1:1470256/76‑1 (MQ=255)        tttttCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTAt                                                           <  2:354574/76‑1 (MQ=255)         ttttCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATc                                                          >  1:1470711/1‑76 (MQ=255)           ttCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATccc                                                        >  1:210745/1‑76 (MQ=255)             cAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCt                                                      <  1:1356093/76‑1 (MQ=255)              aaGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTg                                                     <  1:96844/76‑1 (MQ=255)                gCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCa                                                   <  1:120898/76‑1 (MQ=255)                 cTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCAt                                                  <  1:231649/76‑1 (MQ=255)                  tGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATc                                                 <  1:445266/76‑1 (MQ=255)                   gCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCg                                                >  1:361314/1‑76 (MQ=255)                   gCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCg                                                >  1:621899/1‑76 (MQ=255)                    ccAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGa                                               >  2:1359011/1‑76 (MQ=255)                      aaTGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGAcc                                             >  2:581765/1‑76 (MQ=255)                       aTGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCg                                            <  1:1352185/76‑1 (MQ=255)                          aTTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAAt                                         <  1:1402360/76‑1 (MQ=255)                           tttGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATa                                        <  2:1616343/76‑1 (MQ=255)                           tttGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATa                                        <  1:506137/76‑1 (MQ=255)                           tttGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATa                                        <  1:776941/76‑1 (MQ=255)                           tttGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATa                                        <  2:638635/76‑1 (MQ=255)                           tttGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATa                                        <  2:1731748/76‑1 (MQ=255)                            ttGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATAc                                       >  2:50184/1‑76 (MQ=255)                             tGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATAcc                                      >  1:1618834/1‑76 (MQ=255)                                 ttAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCgag                                  >  1:1272828/1‑76 (MQ=255)                                 ttAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCgag                                  >  1:417521/1‑76 (MQ=255)                                   aaaCACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGAt                                <  1:1034040/76‑1 (MQ=255)                                   aaaCACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGAt                                <  1:59226/76‑1 (MQ=255)                                   aaaCACACAGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAAAACCCgcgac                                >  2:1732613/1‑75 (MQ=255)                                     acacacAGAAAATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCa                              <  1:1578540/76‑1 (MQ=255)                                        cacaGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCTAGATCatat                           <  2:1505760/76‑1 (MQ=255)                                         acaGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATAtg                          <  2:926068/76‑1 (MQ=255)                                           aGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATAtgct                        >  1:313286/1‑76 (MQ=255)                                           aGAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATAtgct                        >  2:117284/1‑76 (MQ=255)                                            taaTATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATAtgctg                       >  2:17746/2‑76 (MQ=255)                                            gAATATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATAtgctg                       <  2:1125244/76‑1 (MQ=255)                                             aaTATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATAtgctgc                      >  2:67330/1‑76 (MQ=255)                                             aaTATATGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATAtgctgc                      >  1:1116548/1‑76 (MQ=255)                                               tatatGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCtt                    <  2:1441848/76‑1 (MQ=255)                                                atatGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTg                   <  2:220082/76‑1 (MQ=255)                                                atatGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTg                   >  1:1468043/1‑76 (MQ=255)                                                  atGTGGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAg                 >  2:502133/1‑76 (MQ=255)                                                   tgtgGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAgg                >  2:134917/1‑76 (MQ=255)                                                     tgGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGAt              >  2:961231/1‑76 (MQ=255)                                                     tgGTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGAt              <  1:1213587/76‑1 (MQ=255)                                                      ggTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGAtt             >  2:1200539/1‑76 (MQ=255)                                                      ggTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGAtt             >  1:1296356/1‑76 (MQ=255)                                                      ggTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGAtt             >  1:1540301/1‑76 (MQ=255)                                                       gTTTCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGAttt            <  2:258462/76‑1 (MQ=255)                                                         ttCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCt          >  2:388019/1‑76 (MQ=255)                                                         ttCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCt          >  1:159515/1‑76 (MQ=255)                                                         ttCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCt          <  2:1418277/76‑1 (MQ=255)                                                          tCGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTa         >  1:512437/1‑76 (MQ=255)                                                           cGAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTAc        >  2:647793/1‑76 (MQ=255)                                                            gAATGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTAcc       >  1:1479908/1‑76 (MQ=255)                                                             aaTGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTACCg      <  1:3632/76‑1 (MQ=255)                                                             aaTGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTACCg      <  1:1241450/76‑1 (MQ=255)                                                             aaTGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATAATATGCTGCTTGAGGATTTCTACCg      >  2:767410/1‑76 (MQ=255)                                                               tGTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTACCGTa    >  1:1035649/1‑76 (MQ=255)                                                                gTTTTTCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTACCGTaa   <  1:1490110/76‑1 (MQ=255)                                                                 tttttCGACTGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTACCGTAAt  >  2:1186623/1‑76 (MQ=255)                                                                          |                                                                   AACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTTTCGACCGACGATTATCCCCTGCATCGACCGAATACCCGAGATCATATGCTGCTTGAGGATTTCTACCGTAAT  >  REL606/4347052‑4347191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑