Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,165,556	C→T	G96S (GGC→AGC)	yehU ←	predicted sensory kinase in two‑component system with YehT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,165,556	0	C	T	100.0%	97.9 / NA	27	G96S (GGC→AGC)	yehU	predicted sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/11);  total (16/11)

AGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCC‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACG  >  REL606/2165483‑2165628                                                                          |                                                                          aGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAgct‑gc                                                                        >  1:1243165/1‑76 (MQ=255)      tCCTTCAACGATGGTTGAGATNATGCTACTTAGCGCGGCCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTc                                                                   <  2:283915/76‑1 (MQ=255)       ccTTCAACGATGGTTGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCa                                                                  <  2:998344/76‑1 (MQ=255)            aaCGATGGTTGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGacca                                                             <  2:743075/76‑1 (MQ=255)                 tGGTTGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAg                                                        <  1:1303510/76‑1 (MQ=255)                 tGGTTGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAg                                                        <  1:41612/76‑1 (MQ=255)                     tGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCa                                                    >  2:1381423/1‑76 (MQ=255)                             tGCAACTTAGCGCGGTCATTCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCgacgac                                            >  1:1238336/1‑76 (MQ=255)                             tGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCgacgac                                            >  2:1264769/1‑76 (MQ=255)                               cAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACgaccg                                          >  1:907661/1‑76 (MQ=255)                                            tCATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTaa                             >  2:1014938/1‑76 (MQ=255)                                             cATGCCCCCCATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                            >  2:1727008/1‑76 (MQ=255)                                              atcccccccACCGAAAATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgc                           <  1:1229448/73‑1 (MQ=255)                                                  cccccATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                       >  2:989538/1‑76 (MQ=255)                                                   ccccATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGccc                      <  1:1190968/76‑1 (MQ=255)                                                   ccccATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGccc                      >  1:1278179/1‑76 (MQ=255)                                                   ccccATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGccc                      >  2:1267398/1‑76 (MQ=255)                                                   ccccATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGccc                      <  1:1611086/76‑1 (MQ=255)                                                     ccATCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCAt                    >  2:1443993/1‑76 (MQ=255)                                                      cATCGAATATCGATGTACGCT‑GCCGGTCAGACCAACCAGCCCCCCGACGACCGGACCGCCGAGTAAGCCGCCCAtt                   <  2:1676108/76‑1 (MQ=255)                                                        tCGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAc                 <  1:869943/76‑1 (MQ=255)                                                         cGAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                >  1:1387790/1‑76 (MQ=255)                                                         cGAATATCGATG‑AAGCCTGCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                <  1:1158789/76‑1 (MQ=255)                                                          gAATATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg               >  2:990210/1‑76 (MQ=255)                                                           aaTATCGATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgc              >  1:670937/1‑76 (MQ=255)                                                                cGATGTAAGCT‑GCCGGTCAGACCAACCAGTCCACCGACGACCGGATCGCCGAGTAAGCCGCCCATTACCGCGCCTa         <  2:295283/76‑1 (MQ=255)                                                                 gATGTAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTAt        >  1:1667823/1‑76 (MQ=255)                                                                     tAAGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa    >  2:1478039/1‑76 (MQ=255)                                                                      aaGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCAc   <  2:444937/76‑1 (MQ=255)                                                                       aGCT‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACg  >  1:772902/1‑76 (MQ=255)                                                                          |                                                                          AGCAATCCTTCAACGATGGTTGAGATCATGCAACTTAGCGCGGTCATGCCCCCCATCGAATATCGATGTAAGCC‑GCCGGTCAGACCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACG  >  REL606/2165483‑2165628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑