| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 475,161 | C→T | G49S (GGC→AGC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 475,161 | 0 | C | T | 97.6% | 154.0 / ‑5.9 | 42 | G49S (GGC→AGC) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (21/20); minor base G (0/1); total (21/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGG > REL606/475091‑475232 | aCGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAg > 2:1104772/1‑76 (MQ=255) cGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGt < 1:656102/76‑1 (MQ=255) aaaCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAAt > 2:742012/1‑76 (MQ=255) aaaCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAAt < 1:1317328/76‑1 (MQ=255) aaaCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAAt < 2:1066073/76‑1 (MQ=255) aaaCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAAt < 1:263996/76‑1 (MQ=255) aTCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTc > 2:1087139/1‑76 (MQ=255) aaCAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTg > 2:645812/1‑76 (MQ=255) aaCAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTg < 2:1059729/76‑1 (MQ=255) aGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCa > 1:880679/1‑76 (MQ=255) gAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAg > 2:594428/1‑76 (MQ=255) tGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGcac > 1:710563/1‑76 (MQ=255) aCGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCacac < 2:97112/76‑1 (MQ=255) cAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCt > 1:1466423/1‑76 (MQ=255) gTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTaa < 1:570083/76‑1 (MQ=255) ttCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAAc < 1:728544/76‑1 (MQ=255) tCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACa > 1:615073/1‑76 (MQ=255) tCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACa < 2:655638/76‑1 (MQ=255) cAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAg > 1:875630/1‑76 (MQ=255) aGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGa > 1:825291/1‑76 (MQ=255) gCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGat < 1:1502765/76‑1 (MQ=255) ccAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGata < 1:878638/76‑1 (MQ=255) ccAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGata > 1:1163844/1‑76 (MQ=255) aGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATc < 1:119654/76‑1 (MQ=255) tCCGGGGCAAGCTTGGGATCGGCAACCAAGCTCGGAGCGGAGGGACCTGCCAGCGCACCCGCTAACAGATATCcca > 2:1231368/1‑76 (MQ=255) cGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCccacc < 1:848900/76‑1 (MQ=255) cGGGGCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCccacc > 2:653242/1‑76 (MQ=255) ggggCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCccacca > 1:1574103/1‑76 (MQ=255) gggCAAGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCag < 1:1052532/76‑1 (MQ=255) atgCTTGGAATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAgagga < 2:317829/74‑1 (MQ=255) aGCTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAgaggag > 1:917100/1‑76 (MQ=255) cTTGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaa > 2:824007/1‑76 (MQ=255) ttGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaaa > 2:925826/1‑76 (MQ=255) ttGGTATCGGCAACAAAGCGCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaaa < 1:490046/76‑1 (MQ=255) tGGTATCGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCACACCAGAGGAGAAAt < 1:1154649/76‑1 (MQ=255) cGGCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAg < 1:1105074/76‑1 (MQ=255) ggCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACAACCGCTAACAGACATCCNACCAGAGGAGAAATACGTAGt > 2:264934/1‑76 (MQ=255) gCAACAAAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGtt < 1:413566/76‑1 (MQ=255) aacaaAGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTa < 1:1262563/76‑1 (MQ=255) aaaGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTg > 2:167123/1‑76 (MQ=255) aaaGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTg > 2:1227224/1‑76 (MQ=255) aaGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTgg > 2:669272/1‑76 (MQ=255) aaGCTCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTgg < 1:244487/76‑1 (MQ=255) | ACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGG > REL606/475091‑475232 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |