| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,928,601 | A→G | N129D (AAC→GAC) | rho → | transcription termination factor Rho |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,928,601 | 0 | A | G | 98.1% | 214.7 / ‑4.2 | 52 | N129D (AAC→GAC) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (32/19); total (33/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTT > REL606/3928528‑3928673 | gAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcgac < 2:1184438/76‑1 (MQ=255)gAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcgac > 2:1534188/1‑76 (MQ=255) aaaGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcgaca > 2:1384093/1‑76 (MQ=255) agaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACaaa < 1:40574/76‑1 (MQ=255) agaagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACaaa > 1:1644625/1‑76 (MQ=255) tGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCctc > 1:1031147/1‑76 (MQ=255) tGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCctc > 2:1523730/1‑76 (MQ=255) tGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCctc < 2:898664/76‑1 (MQ=255) gAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCctct > 1:389599/1‑76 (MQ=255) gAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCctct > 1:1300499/1‑76 (MQ=255) aaCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCtt > 2:1447820/1‑76 (MQ=255) aCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCttt < 2:687784/76‑1 (MQ=255) cTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTgag < 1:296370/76‑1 (MQ=255) ttttttGCGCTGCTAAAATTTAACAAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTgaga < 1:1146271/74‑1 (MQ=255) tATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTgaga > 1:491892/1‑76 (MQ=255) tATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTgaga < 2:1238234/76‑1 (MQ=255) aTTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGaa > 1:295316/1‑76 (MQ=255) aTTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGaa < 2:120685/76‑1 (MQ=255) tttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACt > 2:1376432/1‑76 (MQ=255) tttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACt > 1:674868/1‑76 (MQ=255) tGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTa < 2:289132/76‑1 (MQ=255) gcgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTaa > 1:1442141/1‑76 (MQ=255) gcgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTaa < 2:1143266/76‑1 (MQ=255) gAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTg > 2:747810/1‑76 (MQ=255) gAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTg > 2:1337386/1‑76 (MQ=255) gAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTg > 2:506539/1‑76 (MQ=255) gAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTg > 2:282126/1‑76 (MQ=255) aaaGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGc > 1:799095/1‑76 (MQ=255) aaGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCa < 1:695500/76‑1 (MQ=255) aGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc > 2:1034224/1‑76 (MQ=255) aGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc < 1:49945/76‑1 (MQ=255) gTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACg > 2:1403376/1‑76 (MQ=255) ttAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGc > 1:39186/1‑76 (MQ=255) aaCGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa > 1:834827/1‑76 (MQ=255) aaCGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa < 2:790667/76‑1 (MQ=255) cGAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAc > 2:1559948/1‑76 (MQ=255) gAAGTTAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAct > 2:943710/1‑76 (MQ=255) ttAACTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg < 2:738588/76‑1 (MQ=255) aaCTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTc > 2:252801/1‑76 (MQ=255) aaCTTCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTc > 1:1451827/1‑76 (MQ=255) ttCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc < 1:1534188/76‑1 (MQ=255) ttCGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc > 2:841601/1‑76 (MQ=255) cGACAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGt > 1:181702/1‑76 (MQ=255) cAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATg > 1:1132908/1‑76 (MQ=255) cAAACCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATg < 1:710923/76‑1 (MQ=255) aaCCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGa > 2:1056844/1‑76 (MQ=255) aCCTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGaa < 1:1642904/76‑1 (MQ=255) cTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg < 2:1515860/76‑1 (MQ=255) cTGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg > 1:447167/1‑76 (MQ=255) tGAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGt > 2:1115321/1‑76 (MQ=255) gAAAACGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg > 2:934833/1‑76 (MQ=255) gAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg > 1:708528/1‑76 (MQ=255) aaaCGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGt < 1:1565454/76‑1 (MQ=255) aCGCCCGCGACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTaa > 1:923053/1‑76 (MQ=255) aCGCCCGCGACAAAAACCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTaa < 2:324544/76‑1 (MQ=255) cgcgACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGtt < 2:458501/76‑1 (MQ=255) cgcgACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGtt > 2:1088206/1‑76 (MQ=255) | GAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTT > REL606/3928528‑3928673 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |