Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 4,164,583 T→G M1243R (ATG→AGG)  rpoB → DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,164,5830TG100.0% 196.8 / NA 47M1243R (ATG→AGG) rpoBDNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/22);  total (25/22)

AGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGG  >  REL606/4164514‑4164655
                                                                     |                                                                        
aGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCAcgc                                                                    <  1:11854/76‑1 (MQ=255)
aGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCAcgc                                                                    <  1:601782/76‑1 (MQ=255)
aGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCAcgc                                                                    <  1:273784/76‑1 (MQ=255)
    cGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGt                                                                >  2:448738/1‑76 (MQ=255)
     gAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGtt                                                               >  2:1065591/1‑76 (MQ=255)
     gAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGtt                                                               <  2:1492242/76‑1 (MQ=255)
         gTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCAc                                                           >  2:1098756/1‑76 (MQ=255)
          tCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCAcc                                                          <  1:1108742/76‑1 (MQ=255)
           ccGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCg                                                         <  1:1427727/76‑1 (MQ=255)
           ccGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCg                                                         >  1:777840/1‑76 (MQ=255)
           ccGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCg                                                         <  2:900250/76‑1 (MQ=255)
             ggTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGt                                                       >  2:1042104/1‑76 (MQ=255)
              gTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGtt                                                      <  2:1415431/76‑1 (MQ=255)
               tAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTGCCACCGGTTc                                                     >  1:59222/1‑76 (MQ=255)
                aaCCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCt                                                    <  2:970953/76‑1 (MQ=255)
                 aCCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCtt                                                   <  1:556179/76‑1 (MQ=255)
                    gTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACa                                                <  1:1450218/76‑1 (MQ=255)
                     ttGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAg                                               >  1:1084304/1‑76 (MQ=255)
                      tGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGc                                              >  2:1410284/1‑76 (MQ=255)
                         ttACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTg                                           >  1:1356505/1‑76 (MQ=255)
                          tacatgtacatgCTGAAACTGANCCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTgg                                          >  2:217877/1‑76 (MQ=255)
                           acatgtacatgCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGt                                         >  2:409396/1‑76 (MQ=255)
                           acatgtacatgCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGt                                         >  1:793756/1‑76 (MQ=255)
                               gtacatgCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACt                                     >  1:577327/1‑76 (MQ=255)
                                 acatgCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTca                                   <  2:1280892/76‑1 (MQ=255)
                                  catgCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTcag                                  <  2:48199/76‑1 (MQ=255)
                                  catgCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTcag                                  >  2:391130/1‑76 (MQ=255)
                                        gAAACTGAACCACCTGGTCGACAACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCg                            >  1:616082/1‑76 (MQ=255)
                                            cTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTgg                        <  2:807361/76‑1 (MQ=255)
                                             tGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTggg                       >  1:981836/1‑76 (MQ=255)
                                             tGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTggg                       >  1:912977/1‑76 (MQ=255)
                                              gAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGggt                      >  2:1520466/1‑76 (MQ=255)
                                               aaCCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTgggcg                     >  2:171829/1‑74 (MQ=255)
                                                  caccTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTa                  <  1:1368175/76‑1 (MQ=255)
                                                   cccTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTaa                 <  2:402333/75‑1 (MQ=255)
                                                    ccTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAg                <  1:512280/76‑1 (MQ=255)
                                                     cTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAgg               <  1:1488213/76‑1 (MQ=255)
                                                      tGGTCGACGACAAGAGGCACGCGCGTTCCACCGGCTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGc              >  1:140327/1‑76 (MQ=255)
                                                       ggTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGca             <  2:1088626/76‑1 (MQ=255)
                                                        gTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGcac            <  1:1317138/76‑1 (MQ=255)
                                                          cgacgaCAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAg          <  2:1432558/76‑1 (MQ=255)
                                                           gacgacAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGt         >  1:547756/1‑76 (MQ=255)
                                                           gacgacAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGt         >  1:1281375/1‑76 (MQ=255)
                                                           gacgacAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGt         >  1:1392749/1‑76 (MQ=255)
                                                            acgacAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGtt        >  2:796534/1‑76 (MQ=255)
                                                               acAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCgg     <  2:981836/76‑1 (MQ=255)
                                                               acAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCgg     >  1:417818/1‑76 (MQ=255)
                                                                 aaGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCggtg   <  2:1622196/76‑1 (MQ=255)
                                                                  agagGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCggtgg  >  2:208932/1‑76 (MQ=255)
                                                                     |                                                                        
AGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGG  >  REL606/4164514‑4164655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑