Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,329,516	0	C	T	100.0%	158.7 / NA	41	H33Y (CAC→TAC)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/24);  total (17/24)

TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329443‑1329588                                                                          |                                                                         tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac                                                                        >  2:542248/1‑76 (MQ=255)  gAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtaca                                                                       >  2:1039730/1‑76 (MQ=255)         cGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGa                                                                >  1:870253/1‑76 (MQ=255)              aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc                                                           >  2:1341108/1‑76 (MQ=255)                aGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCg                                                         <  1:1506381/76‑1 (MQ=255)                 gCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGa                                                        <  2:1532555/76‑1 (MQ=255)                  ccAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAc                                                       >  1:1230476/1‑76 (MQ=255)                    aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCa                                                     <  2:689249/76‑1 (MQ=255)                    aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCa                                                     >  1:70594/1‑76 (MQ=255)                     aaaCGATCAACAAGTATCTGGGTAGCGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAg                                                    >  2:957435/1‑76 (MQ=255)                       aCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTg                                                  <  1:1503595/76‑1 (MQ=255)                          aTCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCt                                               <  1:512740/76‑1 (MQ=255)                           tCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTc                                              <  2:1583786/76‑1 (MQ=255)                             aacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAg                                            >  2:1257352/1‑76 (MQ=255)                              acaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTATATCCGCGATTTGCCGACCAGTGGCTCAGc                                           >  2:277124/1‑76 (MQ=255)                              acaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGc                                           <  2:680/76‑1 (MQ=255)                              acaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGc                                           <  2:487291/76‑1 (MQ=255)                               caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          <  2:457603/76‑1 (MQ=255)                                aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTg                                         <  2:1042591/76‑1 (MQ=255)                                 aGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGc                                        <  1:1094369/76‑1 (MQ=255)                                 aGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCCCGATTTGCCGACCAGTGGCTCAGCTGc                                        >  2:1219097/1‑76 (MQ=255)                                    aTCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaa                                     <  1:408225/76‑1 (MQ=255)                                    aTCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaa                                     <  2:206208/76‑1 (MQ=255)                                    aTCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaa                                     <  2:206202/76‑1 (MQ=255)                                      cTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaa                                   >  1:1436583/1‑76 (MQ=255)                                        gggTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCCCGATTTGCCGACCCACGGCTCACCTGCCaaaaaa                                 >  1:580611/1‑75 (MQ=255)                                            aGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTg                             <  2:1111875/76‑1 (MQ=255)                                             gTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGc                            <  1:1176254/76‑1 (MQ=255)                                              tGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGGGcg                           >  2:97227/1‑75 (MQ=255)                                               gACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGcag                          <  2:1487195/76‑3 (MQ=255)                                                 cgaCGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAc                        <  2:144085/74‑1 (MQ=255)                                                   aCGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActc                      <  2:678076/76‑1 (MQ=255)                                                     gtggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa                    <  2:98872/76‑1 (MQ=255)                                                          gAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTcc               >  2:526375/1‑76 (MQ=255)                                                          gAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTcc               <  1:1243473/76‑1 (MQ=255)                                                           aaaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTcca              >  2:397978/1‑76 (MQ=255)                                                            aaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccac             <  2:1207083/76‑1 (MQ=255)                                                             aTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacc            >  1:1230333/1‑76 (MQ=255)                                                               ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa          >  2:1280654/1‑76 (MQ=255)                                                                cggCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         <  1:716989/74‑1 (MQ=255)                                                                cAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         >  1:729843/1‑76 (MQ=255)                                                                cAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         <  1:492641/76‑1 (MQ=255)                                                                cAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         <  1:1168900/76‑1 (MQ=255)                                                                      cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTTTACCTCCACCAAGAAGGCt   >  2:406739/1‑76 (MQ=255)                                                                       ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  <  2:1502018/76‑1 (MQ=255)                                                                          |                                                                         TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329443‑1329588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑