Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,488,837	T→A	N124Y (AAT→TAT)	glpR ←	DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,488,837	0	T	A	100.0%	208.1 / NA	51	N124Y (AAT→TAT)	glpR	DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (36/15);  total (36/15)

TCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCTTCCGGCGTGGTGC  >  REL606/3488764‑3488908                                                                          |                                                                        tCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAga                                                                       <  1:1266760/76‑1 (MQ=255)  cgcgGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATagag                                                                      >  2:1616349/1‑76 (MQ=255)        tGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGAttgtt                                                                <  1:250835/76‑1 (MQ=255)           gTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGt                                                             >  1:1138241/1‑76 (MQ=255)           gTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGt                                                             >  1:493759/1‑76 (MQ=255)           gTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGt                                                             >  1:1498252/1‑76 (MQ=255)            tAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTg                                                            >  1:1296333/1‑76 (MQ=255)            tAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTg                                                            <  1:503735/76‑1 (MQ=255)             aaTTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGa                                                           <  1:1561893/76‑1 (MQ=255)                tCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACaa                                                        >  2:998595/1‑76 (MQ=255)                tCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACaa                                                        >  2:98149/1‑76 (MQ=255)                 cGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAAt                                                       >  2:454494/1‑76 (MQ=255)                 cGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAAt                                                       >  1:1398257/1‑76 (MQ=255)                    cACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGATAATACg                                                    >  2:444649/1‑76 (MQ=255)                    cACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACg                                                    <  1:1552322/76‑1 (MQ=255)                     aCCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGc                                                   >  2:1520138/1‑76 (MQ=255)                     aCCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGc                                                   >  1:1332077/1‑76 (MQ=255)                      ccGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCa                                                  >  1:857183/1‑76 (MQ=255)                       cGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCaa                                                 >  2:1653785/1‑76 (MQ=255)                       cGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCaa                                                 >  2:1159853/1‑76 (MQ=255)                        ggCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCaaa                                                <  1:1361411/76‑1 (MQ=255)                        ggCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCaaa                                                <  1:1440273/76‑1 (MQ=255)                           gAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTg                                             >  1:1439097/1‑76 (MQ=255)                            aaaaTGATGCGGAAATCTTGTTNTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGc                                            >  2:217755/1‑76 (MQ=255)                               atgatgCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCtgt                                         <  2:787580/76‑1 (MQ=255)                                 gatgCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGa                                       >  1:900888/1‑76 (MQ=255)                                   tgCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGAtt                                     <  1:1330783/76‑1 (MQ=255)                                   tgCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGAtt                                     >  1:387836/1‑76 (MQ=255)                                          aTCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAagcagc                              >  1:963178/1‑76 (MQ=255)                                          aTCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAagcagc                              <  1:1524922/76‑1 (MQ=255)                                           tCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAgcg                             >  1:606/1‑76 (MQ=255)                                           tCTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAgcg                             >  2:1264459/1‑76 (MQ=255)                                            cTTGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAccgc                            >  2:1081095/1‑76 (MQ=255)                                             ttGTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGcgcg                           >  1:739038/1‑76 (MQ=255)                                               gTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTg                         <  2:69573/76‑1 (MQ=255)                                               gTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTg                         >  2:436559/1‑76 (MQ=255)                                               gTTTTACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTg                         <  2:1097320/76‑1 (MQ=255)                                                ttttACCATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTgc                        <  2:1610189/76‑1 (MQ=255)                                                      cATCAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCAcc                  >  1:1319896/1‑76 (MQ=255)                                                         cAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCt               >  2:1182270/1‑76 (MQ=255)                                                         cAACGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCt               >  1:1270824/1‑76 (MQ=255)                                                          aaCGTGTTAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCtt              <  2:156325/76‑1 (MQ=255)                                                                ttAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCTTCCGGCg        >  2:1011258/1‑76 (MQ=255)                                                                ttAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCTTCCGGCg        >  2:648285/1‑76 (MQ=255)                                                                 tAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCTTCCGGCgt       >  1:867624/1‑76 (MQ=255)                                                                 tAGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCTTCCGGCgt       >  1:1422770/1‑76 (MQ=255)                                                                  aGCAACATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCTTCCGGCgtg      >  1:176677/1‑76 (MQ=255)                                                                     aaCATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCTTCCGGCgtggtg   >  2:1509008/1‑76 (MQ=255)                                                                     aaCATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCTTCCGGCgtggtg   <  2:1465499/76‑1 (MQ=255)                                                                     aaCATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCTTCCGGCgtggtg   >  2:189677/1‑76 (MQ=255)                                                                     aaCATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCTTCCGGCgtggtg   >  1:1299719/1‑76 (MQ=255)                                                                      aCATAGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCTTCCGGCGTGGTGc  >  1:755265/1‑76 (MQ=255)                                                                          |                                                                        TCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGCCACCGCTTCCGGCGTGGTGC  >  REL606/3488764‑3488908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑