Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,131,466	(A)9→8	intergenic (‑125/+175)	gatY ← / ← fbaB	D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,131,458	0	A	.	100.0%	173.9 / NA	35	intergenic (‑117/+183)	gatY/fbaB	D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (29/6);  total (29/6)

AATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAC  >  REL606/2131384‑2131528                                                                           |                                                                       aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa                                                                       >  2:1494137/1‑76 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa                                                                       <  1:1422532/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa                                                                       >  1:1632247/1‑76 (MQ=255)  atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa                                                                      >  2:1318411/1‑76 (MQ=255)  atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa                                                                      >  1:359394/1‑76 (MQ=255)  atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa                                                                      >  1:1346086/1‑76 (MQ=255)   tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa                                                                     <  1:1291868/76‑1 (MQ=255)   tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa                                                                     <  1:563018/76‑1 (MQ=255)    gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa                                                                    >  1:1589923/1‑76 (MQ=255)     aTTTGTTTTTAAATGTCGAGATNACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa                                                                   >  2:299664/1‑76 (MQ=255)     aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa                                                                   <  1:1346605/76‑1 (MQ=255)       ttGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaa                                                                 >  1:2065147/1‑76 (MQ=255)         gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg                                                               <  2:823569/76‑3 (MQ=255)          tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg                                                              <  1:246220/76‑4 (MQ=255)          tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg                                                              >  1:2184585/1‑73 (MQ=255)          tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg                                                              >  1:634881/1‑73 (MQ=255)          tttttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacgg                                                              >  1:119585/1‑73 (MQ=255)            tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc                                                           >  1:1328309/1‑76 (MQ=255)            tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGcc                                                           >  1:77612/1‑76 (MQ=255)             ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt                                                          <  1:1518614/76‑1 (MQ=255)              tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc                                                         >  2:317980/1‑76 (MQ=255)                 aTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCg                                                      >  1:527440/1‑76 (MQ=255)                  tGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCgg                                                     >  1:371853/1‑76 (MQ=255)                   gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGa                                                    >  1:1587761/1‑76 (MQ=255)                    tCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAg                                                   >  1:1725921/1‑76 (MQ=255)                    tCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAg                                                   <  1:603820/76‑1 (MQ=255)                     cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg                                                  >  2:870242/1‑76 (MQ=255)                     cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg                                                  >  2:1547460/1‑76 (MQ=255)                      gagaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAggg                                                 <  2:1225209/76‑1 (MQ=255)                       agaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGa                                                >  1:1164261/1‑76 (MQ=255)                        gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGaa                                               >  1:1228812/1‑76 (MQ=255)                               aTCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaa                                        >  1:1698629/1‑76 (MQ=255)                                 cacaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaaca                                      >  1:299779/1‑76 (MQ=255)                                  acaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGcaacaa                                     >  2:1444606/1‑76 (MQ=255)                                     aaaaCAAAAATAAAAAAATAAAAAGA‑GAAAGAAAAGAAAAAAAAACCGGCCCCCCGGAGGGAAGCCGCAACAAAGt                                  <  1:1590452/76‑1 (MQ=255)                                      aaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTg                                 <  2:2024626/76‑1 (MQ=255)                                       aaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTgc                                >  1:1258285/1‑76 (MQ=255)                                               tatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtt                        >  2:3673/1‑76 (MQ=255)                                               tatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCCCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGtt                        <  2:1998930/76‑1 (MQ=255)                                                  gAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTa                     >  2:1118033/1‑76 (MQ=255)                                                   aaaaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTAt                    >  1:1148422/1‑76 (MQ=255)                                                     aaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGc                  >  1:1914832/1‑76 (MQ=255)                                                      aTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCa                 <  1:2147126/76‑1 (MQ=255)                                                       ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg                >  2:667026/1‑76 (MQ=255)                                                       ttAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAg                >  2:902455/1‑76 (MQ=255)                                                        tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTTCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg               >  1:2104247/1‑76 (MQ=255)                                                        tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg               >  2:901501/1‑76 (MQ=255)                                                          aTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt             >  1:1414454/1‑76 (MQ=255)                                                              agGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGGTTTATGCAGGATgcgg         >  1:418357/1‑76 (MQ=255)                                                                gAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGGTTTATGCAGGATgcggcg       >  1:171846/1‑76 (MQ=39)                                                                 aaTGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt      >  1:1694060/1‑76 (MQ=255)                                                                   tGAAAGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa    <  1:231773/76‑1 (MQ=255)                                                                   tGAAAGG‑AAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa    <  2:1970320/76‑1 (MQ=255)                                                                     aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc  >  1:2192309/1‑76 (MQ=255)                                                                     aaaGGC‑AAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAc  >  1:2070469/1‑76 (MQ=255)                                                                           |                                                                       AATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGGGAAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAAC  >  REL606/2131384‑2131528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑