| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,043,426 | 0 | T | A | 21.0% | 161.7 / 11.2 | 63 | I200F (ATT→TTT) | yihO | predited transporter |
| Reads supporting (aligned to +/- strand): ref base T (31/18); new base A (0/13); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.96e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TACTGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGC > REL606/4043351‑4043496 | tACTGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAAt > 1:761601/1‑76 (MQ=255)tACTGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAAt < 1:1475751/76‑1 (MQ=255)tACTGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAAt > 2:1618650/1‑76 (MQ=255) aCTGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATa < 2:1154079/76‑1 (MQ=255) aCTGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATa > 1:1875959/1‑76 (MQ=255) cTGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATaa > 2:1644180/1‑76 (MQ=255) tGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATaaa < 2:795855/76‑1 (MQ=255) tGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATaaa < 1:1467175/76‑1 (MQ=255) tGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATaaa > 2:145563/1‑76 (MQ=255) ggCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAg > 1:1398990/1‑76 (MQ=255) ggCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAg > 1:523564/1‑76 (MQ=255) ttATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAgccgc > 2:107150/1‑76 (MQ=255) ttATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAgccgc < 1:1607114/76‑1 (MQ=255) ttATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAgccgc > 1:318662/1‑76 (MQ=255) tATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAgccgcc > 2:2214394/1‑76 (MQ=255) aTGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCa > 1:577434/1‑76 (MQ=255) aTGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCa < 1:667208/76‑1 (MQ=255) aTGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCa < 2:96089/76‑1 (MQ=255) tGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCaa < 2:413809/76‑1 (MQ=255) ccGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATgg > 2:630166/1‑76 (MQ=255) gTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGgaga < 2:277126/76‑1 (MQ=255) gTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCAAAAAAAAAAAGCCGCCAATGgaga < 2:1441602/76‑1 (MQ=255) tGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAAc > 2:2029972/1‑76 (MQ=255) ggTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACa > 1:558530/1‑76 (MQ=255) gTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCAAAAAAATAAAGCCGCCAATGGAGAACAt < 2:1057491/76‑1 (MQ=255) gTGCTGTGCCCACATAATGCTCTTTGACGCCTCTGAAGCACAGCATCAAAAAAATAAAGCCGCCAATGGAGAACAt < 1:1682975/76‑1 (MQ=255) tGCGGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAAAAAAGCCGCCAATGGAGAACATa < 1:470827/76‑1 (MQ=255) tgtgTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGt < 2:318662/76‑1 (MQ=255) gtgtCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAAAAAAGCCGCCAATGGAGAACATAAGTg < 1:1971435/76‑1 (MQ=255) tgtCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGc < 1:782572/76‑1 (MQ=255) tgtCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAAAAAAGCCGCCAATGGAGAACATAAGTGc < 1:1565041/76‑1 (MQ=255) gtCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGcc > 1:964455/1‑76 (MQ=255) cacaTAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGca > 1:2110888/1‑76 (MQ=255) gcaTAATGCTCGTGGGCGCTTCTGTAGCACAGCTTAATAAAAAGAAATCCGCCAATGGAGAACATAAGTGCCGcac < 1:948546/75‑1 (MQ=255) tAATGNTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAgg > 2:22662/1‑76 (MQ=255) tAATGCTCTTTGACGCCTCTGTAGCACAGCATAATAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAgg < 1:903838/76‑1 (MQ=255) aaTGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGc > 1:2188587/1‑76 (MQ=255) aaTGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGc > 1:921738/1‑76 (MQ=255) tGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCAt > 2:521863/1‑76 (MQ=255) tGCTCTTGGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCAt < 2:1734609/76‑1 (MQ=255) gCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATa < 2:1598311/76‑1 (MQ=255) ctttttGACGCCTCTGTAGCACAGCATCAAAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATaa < 1:741189/73‑1 (MQ=255) tctTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAAc > 1:1166577/1‑76 (MQ=255) tctTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAAc > 1:396921/1‑76 (MQ=255) tctTTGACGCCTCTGTAGCACAACATAAAAAAAATAAGGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAAc < 1:734730/76‑1 (MQ=255) ctTTGCCGCCTCTGTAGCACAGCATCATAAAAAGAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAAcc < 2:755161/76‑1 (MQ=255) ctTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAAcc > 2:1135655/1‑76 (MQ=255) ttGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGa > 1:1686340/1‑76 (MQ=255) tGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGAc > 2:993949/1‑76 (MQ=255) tGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGAc > 1:1165662/1‑76 (MQ=255) tGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGAc > 1:5517/1‑76 (MQ=255) tGACGCCTCTGTAGCACAGCATCAAAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGAc < 2:106545/76‑1 (MQ=255) gACGCCTCTGTAGCACAGCATCAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACg < 1:707631/76‑1 (MQ=255) aCGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACgg < 1:164683/76‑1 (MQ=255) gCCTCTGAAGCACAGCATCAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGtt < 1:2154034/76‑1 (MQ=255) ccTCTGTAGCACAGCATCATAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTg < 1:2006966/76‑1 (MQ=255) ccTCTGTAGCACAGCATAAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTg < 1:1522403/76‑1 (MQ=255) tctGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAg > 2:923007/1‑76 (MQ=255) ctGTAGCACAGCATCAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGt < 2:1478033/76‑1 (MQ=255) ctGAAGCAGAGCATAAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGt < 2:1105955/76‑1 (MQ=255) tAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAg < 1:2178759/76‑1 (MQ=255) aGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGa > 1:1256146/1‑76 (MQ=255) caGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGaaaaa > 1:2036618/1‑76 (MQ=255) caGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGaaaaa > 1:1538166/1‑76 (MQ=255) aGCATAAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGaaaaaa < 1:1713883/76‑1 (MQ=255) aGCATAAAAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGaaaaaa < 1:548792/76‑1 (MQ=255) catcatAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGc < 2:1871940/76‑1 (MQ=255) atcatAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGATAAAAGCg > 1:1366754/1‑76 (MQ=255) atcaaAAAAAAAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCg < 1:534584/76‑1 (MQ=255) aaaaaaaaAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGc < 2:2029292/76‑1 (MQ=255) aaaaaTAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGc > 2:1976780/1‑76 (MQ=255) | TACTGGCTTTATGTCCGGTTGGTGCTGTGTCCACATAATGCTCTTTGACGCCTCTGTAGCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGC > REL606/4043351‑4043496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |