Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,163,089	A→C	E745A (GAA→GCA)	rpoB →	DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,163,089	0	A	C	100.0%	301.2 / NA	73	E745A (GAA→GCA)	rpoB	DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (39/34);  total (39/34)

GGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGC  >  REL606/4163016‑4163160                                                                          |                                                                        ggtggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAg                                                                       >  1:1738060/1‑76 (MQ=255)  gtggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGc                                                                      >  2:1637930/1‑76 (MQ=255)  gtggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGc                                                                      <  2:1006878/76‑1 (MQ=255)    ggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGcagcag                                                                    <  2:1876263/76‑1 (MQ=255)     gtgtCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAgg                                                                   <  1:854793/76‑1 (MQ=255)        tCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTAt                                                                >  1:391868/1‑76 (MQ=255)        tCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTAt                                                                <  1:1577082/76‑1 (MQ=255)         cGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTGACGAAGACGAGATGTATTCGGGTGCAGCAGGTATc                                                               <  1:795117/76‑1 (MQ=255)         cGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATc                                                               <  1:1536519/76‑1 (MQ=255)          gTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCg                                                              >  2:594314/1‑76 (MQ=255)           ttCAGTGCGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGa                                                             <  2:1586133/76‑1 (MQ=255)           ttCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGa                                                             >  2:1310880/1‑76 (MQ=255)           ttCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGa                                                             <  1:480539/76‑1 (MQ=255)             cAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACa                                                           >  1:931909/1‑76 (MQ=255)             cAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACa                                                           >  1:1853832/1‑76 (MQ=255)              aGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACAt                                                          >  1:98829/1‑76 (MQ=255)               gTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATc                                                         >  1:1608258/1‑76 (MQ=255)                tACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCt                                                        >  1:305506/1‑76 (MQ=255)                tACGTGGATGCTTCCCGCATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCt                                                        >  1:2148031/1‑76 (MQ=255)                  cGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTAc                                                      <  2:760570/76‑1 (MQ=255)                  cGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTAc                                                      >  1:1972239/1‑76 (MQ=255)                   gTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACa                                                     <  1:148444/76‑1 (MQ=255)                   gTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACa                                                     >  1:1444966/1‑76 (MQ=255)                    tGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGTTATCGACATCTACaa                                                    <  1:1699764/76‑1 (MQ=255)                    tGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACaa                                                    <  2:1713369/76‑1 (MQ=255)                     ggATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAAc                                                   >  2:1049942/1‑76 (MQ=255)                       ntGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCt                                                 >  1:25758/2‑76 (MQ=255)                       aTGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCt                                                 >  1:2192883/1‑76 (MQ=255)                       aTGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCt                                                 >  2:172498/1‑76 (MQ=255)                        tGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTg                                                <  2:1349904/76‑1 (MQ=255)                        tGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTg                                                <  1:1503203/76‑1 (MQ=255)                          cTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGAc                                              <  1:1226937/76‑1 (MQ=255)                          cTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGAc                                              >  2:2089124/1‑76 (MQ=255)                          cTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGGATCGACATCTACAACCTGAc                                              >  1:1810117/1‑76 (MQ=255)                          cTTCCCGTATCGTTATCAAAGTNAACGAAGACGAGATGTATCCGGGTGCAGGAGGTATCGACATGTACAACCTGAc                                              >  2:286794/1‑76 (MQ=255)                           ttCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGAcc                                             <  1:959457/76‑1 (MQ=255)                            tCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCa                                            >  2:1709344/1‑76 (MQ=255)                             cccGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCaa                                           <  1:1754759/76‑1 (MQ=255)                             cccGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCaa                                           >  2:1113230/1‑76 (MQ=255)                               cGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAAt                                         <  1:1324275/76‑1 (MQ=255)                               cGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAAt                                         <  1:2096670/76‑1 (MQ=255)                                gTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATa                                        >  2:175474/1‑76 (MQ=255)                                  aTCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATaca                                      <  2:841557/76‑1 (MQ=255)                                       tATCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGt                                 >  1:1393533/1‑76 (MQ=255)                                         tCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTc                               >  2:1648970/1‑76 (MQ=255)                                         tCAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTc                               <  2:1977014/76‑1 (MQ=255)                                          cAAAGTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCt                              <  1:441961/76‑1 (MQ=255)                                              gTTAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAAcc                          >  2:594867/1‑76 (MQ=255)                                               ttAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCa                         >  2:222223/1‑76 (MQ=255)                                               ttAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCa                         <  1:327155/76‑1 (MQ=255)                                               ttAACGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCa                         >  1:268847/1‑76 (MQ=255)                                                  aCGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGaa                      <  2:1025167/76‑1 (MQ=255)                                                   cGAAGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAac                     <  1:576530/76‑1 (MQ=255)                                                     aaGACGAGATGTATCCGGGTGCAGCAGGTATCGACATGTACAACCTGACTAAATNCACCCGTTCTAACCAGAacac                   >  2:346632/1‑76 (MQ=255)                                                     aaGACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAacac                   <  2:1444966/76‑1 (MQ=255)                                                       gACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCt                 <  1:269708/76‑1 (MQ=255)                                                       gACGAGATGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCt                 <  1:1678374/76‑1 (MQ=255)                                                           agaTGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGGCCAAATACACCCGTTCTAACCAGAACCCCTGTAt             <  2:1718684/76‑1 (MQ=255)                                                            gaTGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATc            <  1:52625/76‑1 (MQ=255)                                                              tGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCaa          <  2:931804/76‑1 (MQ=255)                                                              tGTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCaa          >  1:13442/1‑76 (MQ=255)                                                               gTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAc         >  1:1860343/1‑76 (MQ=255)                                                               gTATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAc         >  2:864179/1‑76 (MQ=255)                                                                tATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc        <  2:1818711/76‑1 (MQ=255)                                                                tATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc        <  2:1741095/76‑1 (MQ=255)                                                                tATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc        >  2:336296/1‑76 (MQ=255)                                                                tATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc        <  1:1049942/76‑1 (MQ=255)                                                                tATCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc        >  1:1032152/1‑76 (MQ=255)                                                                 aTCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCa       >  2:1517423/1‑76 (MQ=255)                                                                 aTCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCa       <  1:1704681/76‑1 (MQ=255)                                                                  tCCGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAg      >  2:337676/1‑76 (MQ=255)                                                                   ccGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGa     <  2:920912/76‑1 (MQ=255)                                                                    cGGGTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGAt    >  1:1038794/1‑76 (MQ=255)                                                                      ggTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGc  >  2:1304516/1‑76 (MQ=255)                                                                      ggTGCAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGc  >  2:591294/1‑76 (MQ=255)                                                                          |                                                                        GGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGC  >  REL606/4163016‑4163160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑