| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 475,302 | Δ1 bp | coding (4/1677 nt) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 475,302 | 0 | G | . | 98.6% | 344.4 / ‑2.3 | 70 | coding (4/1677 nt) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (46/23); total (46/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTAATAG > REL606/475232‑475371 | gCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTcc < 2:1255425/76‑1 (MQ=255)gCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTcc < 2:63243/76‑1 (MQ=255) cAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGt > 2:129579/1‑76 (MQ=255) cAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGt > 2:1909597/1‑76 (MQ=255) aGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGtc > 1:1169233/1‑76 (MQ=255) gCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGtct < 2:328777/76‑1 (MQ=255) gCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATCCCGtct > 2:697047/1‑76 (MQ=255) aTGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTcc > 2:1692306/1‑76 (MQ=255) aTGCAGAGAATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTcc < 1:190219/76‑1 (MQ=255) cGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCttt < 1:680223/76‑1 (MQ=255) gAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTc > 2:144309/1‑76 (MQ=255) aGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTcc < 1:592136/76‑1 (MQ=255) aGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTcc > 2:1534679/1‑76 (MQ=255) aGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTcc > 2:2190377/1‑76 (MQ=255) aTAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCtgg > 1:960149/1‑76 (MQ=255) aTAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCtgg > 1:701903/1‑76 (MQ=255) aTAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCtgg > 2:997368/1‑76 (MQ=255) tAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCtggt > 1:1349426/1‑76 (MQ=255) tAAAGGCGAGCACAAGGCCGACAACAATGGTGGTGATAAGCGGGGGGGCGTGAT‑CATTCCGTCTCCTTTTCCtggt > 2:1419797/1‑76 (MQ=255) aaaGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCtggtg > 2:1781450/1‑76 (MQ=255) aaaGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCtggtg > 2:1303364/1‑76 (MQ=255) aaaGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCtggtg > 2:782414/1‑76 (MQ=255) aaGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCtggtgg > 2:1415398/1‑76 (MQ=255) aGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTggtggt > 2:1749430/1‑76 (MQ=255) gCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTa < 1:1014445/76‑1 (MQ=255) gAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTAtt < 1:376074/76‑1 (MQ=255) gAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTAtt > 2:2236009/1‑76 (MQ=255) cacaAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTc < 1:1437704/76‑1 (MQ=255) cacaAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTc > 2:472274/1‑76 (MQ=255) acaAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTcc > 1:2131921/1‑76 (MQ=255) aaGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCAt > 2:669047/1‑76 (MQ=255) aaGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCAt > 2:1669038/1‑76 (MQ=255) aGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCAtt < 2:1533030/76‑1 (MQ=255) aGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCCTTTTCTGGTGGTTTTTTTCCAtt > 1:1169193/1‑76 (MQ=255) ccgccAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCAttttt > 1:261268/1‑76 (MQ=255) ccgccAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCAttttt > 1:87771/1‑76 (MQ=255) ccgccAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCAttttt < 2:1077917/76‑1 (MQ=255) ccgccAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCAttttt > 1:1868754/1‑76 (MQ=255) ccgccAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCAttttt < 1:2160276/76‑1 (MQ=255) cgccAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTg > 2:1926211/1‑76 (MQ=255) ccAACAATGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGc < 1:78158/76‑1 (MQ=255) caacaaTGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGcc > 2:844288/1‑76 (MQ=255) caacaaTGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGcc > 1:1724298/1‑76 (MQ=255) caacaaTGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGcc > 1:1001397/1‑76 (MQ=255) caacaaTGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGcc > 1:514708/1‑76 (MQ=255) caacaaTGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGcc > 1:2188773/1‑76 (MQ=255) acaaTGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCgg > 1:618093/1‑76 (MQ=255) caaTGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCTATTTTTGGCCggg < 1:597092/76‑1 (MQ=255) caaTGGTGGTGATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCggg > 2:1293525/1‑76 (MQ=255) tggtgATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAAcc < 1:1909597/76‑1 (MQ=255) ggtgATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCa < 1:25172/76‑1 (MQ=255) ggtgATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCa > 1:1865064/1‑76 (MQ=255) gtgATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCaa < 1:1308944/76‑1 (MQ=255) gATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCaaaa > 1:1229807/1‑76 (MQ=255) gATAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCaaaa > 1:1229828/1‑76 (MQ=255) aTAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTTGCCGGGGAAACCAAAAt > 1:1244327/1‑76 (MQ=255) aTAAGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAAt > 2:1486184/1‑76 (MQ=255) aaGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTa < 2:678289/76‑1 (MQ=255) aaGCGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTa > 2:1889463/1‑76 (MQ=255) cGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAg > 1:2191834/1‑76 (MQ=255) cGGGGTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAg > 1:1961605/1‑76 (MQ=255) ggggTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAgg < 2:297921/76‑1 (MQ=255) ggggTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAgg > 2:244344/1‑76 (MQ=255) ggggTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAgg > 2:1654016/1‑76 (MQ=255) gggTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGt > 1:6384/1‑76 (MQ=255) ggTGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTa < 1:1522062/76‑1 (MQ=255) tGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTAAt < 1:941310/76‑1 (MQ=255) tGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTAAt > 1:288548/1‑76 (MQ=255) tGGCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGGAAt > 1:676354/1‑76 (MQ=255) ggCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTAATa < 2:375690/76‑1 (MQ=255) ggCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTAATa < 1:887418/76‑1 (MQ=255) gCGTGAT‑CATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTAATAg < 1:1271626/76‑1 (MQ=255) | GCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTAATAG > REL606/475232‑475371 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |