Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	430,835	C→T	intergenic (‑48/‑108)	insL‑2 ← / → lon	putative transposase insL for insertion sequence IS186/DNA‑binding ATP‑dependent protease La

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	430,835	0	C	T	100.0%	344.9 / NA	89	intergenic (‑48/‑108)	insL‑2/lon	putative transposase insL for insertion sequence IS186/DNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/48);  total (41/48)

TCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTG  >  REL606/430771‑430907                                                                 |                                                                         tCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGa                                                               <  1:534682/76‑1 (MQ=14)  cGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGaa                                                              <  1:2179410/76‑1 (MQ=17)  cGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGaa                                                              <  2:524319/76‑1 (MQ=17)  cGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGaa                                                              <  2:519582/76‑1 (MQ=17)  cGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGaa                                                              >  1:770487/1‑76 (MQ=17)  cGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGaa                                                              >  2:2137380/1‑76 (MQ=17)   gTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGaaa                                                             <  1:2010381/76‑1 (MQ=21)   gTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGaaa                                                             <  1:665805/76‑1 (MQ=21)    tGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAAc                                                            >  2:111817/1‑76 (MQ=25)      agagTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACAt                                                          <  2:977963/76‑1 (MQ=255)      agagTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACAt                                                          >  2:498175/1‑76 (MQ=255)      agagTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACAt                                                          >  2:920113/1‑76 (MQ=255)      agagTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACAt                                                          >  1:1032290/1‑76 (MQ=255)         gTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATccc                                                       >  2:890255/1‑76 (MQ=255)         gTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATccc                                                       >  1:2212322/1‑76 (MQ=255)             ttCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCata                                                   <  1:839695/76‑1 (MQ=255)             ttCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCata                                                   >  1:684353/1‑76 (MQ=255)             ttCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCata                                                   <  1:41265/76‑1 (MQ=255)              tCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCatat                                                  <  2:1660263/76‑1 (MQ=255)              tCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCatat                                                  <  2:334195/76‑1 (MQ=255)              tCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCatat                                                  <  2:985393/76‑1 (MQ=255)              tCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCatat                                                  <  1:1294004/76‑1 (MQ=255)              tCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCatat                                                  >  1:1109205/1‑76 (MQ=255)              tCATCGGCACGTCAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCatat                                                  <  2:343910/76‑1 (MQ=255)               cATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCAtata                                                 <  1:1825302/76‑1 (MQ=255)               cATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCAtata                                                 <  2:655067/76‑1 (MQ=255)               cATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCAtata                                                 <  1:466018/76‑1 (MQ=255)                aTCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATAc                                                >  1:2022596/1‑76 (MQ=255)                aTCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATAc                                                >  1:2040843/1‑76 (MQ=255)                aTCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATAc                                                >  1:1837359/1‑76 (MQ=255)                 tCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACt                                               <  2:1524806/76‑1 (MQ=255)                  cGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTg                                              <  1:1549206/76‑1 (MQ=255)                  cGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTg                                              >  2:829179/1‑76 (MQ=255)                   ggCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGa                                             <  1:2230490/76‑1 (MQ=255)                   ggCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGa                                             <  2:1032290/76‑1 (MQ=255)                    gCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGAc                                            <  2:1056743/76‑1 (MQ=255)                     cACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACg                                           >  1:392836/1‑76 (MQ=255)                     cACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACg                                           >  2:1540565/1‑76 (MQ=255)                     cACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACg                                           >  2:151951/1‑76 (MQ=255)                      aCGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGt                                          >  2:772565/1‑76 (MQ=255)                      aCGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGt                                          >  1:1700947/1‑76 (MQ=255)                      aCGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGt                                          <  1:1008676/76‑1 (MQ=255)                       cGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTa                                         <  1:1003837/76‑1 (MQ=255)                       cGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTa                                         <  2:1621987/76‑1 (MQ=255)                       cGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTa                                         >  1:1846696/1‑76 (MQ=255)                       cGTTAAATCATAACAGGCGTAATTCCACATCCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTa                                         <  1:33887/76‑1 (MQ=255)                         ttAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACa                                       >  1:2230917/1‑76 (MQ=255)                           aaaTCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATg                                     >  1:11178/1‑76 (MQ=255)                           aaaTCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATg                                     <  1:1392030/76‑1 (MQ=255)                           aaaTCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATg                                     >  2:597810/1‑76 (MQ=255)                           aaaTCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATg                                     <  2:1506405/76‑1 (MQ=255)                            aaTCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGt                                    <  1:465717/76‑1 (MQ=255)                             aacATAACAGGCGGAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGtt                                   <  1:514205/74‑1 (MQ=25)                             aTCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGtt                                   <  2:1968362/76‑1 (MQ=255)                                atatCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAAt                                >  1:1375433/1‑76 (MQ=255)                                 tatCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATATTGACGTACATGTTAATa                               <  1:930297/76‑1 (MQ=255)                                   tCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGa                             >  2:190451/1‑76 (MQ=255)                                   tCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGa                             >  2:137946/1‑76 (MQ=255)                                    cAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGAt                            <  1:1181085/76‑1 (MQ=255)                                    cAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGAt                            >  2:1509003/1‑76 (MQ=255)                                     aGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGATAATAGATg                           >  2:656231/1‑76 (MQ=255)                                       gCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGc                         <  2:547785/76‑1 (MQ=255)                                        cGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCg                        <  1:864222/76‑1 (MQ=255)                                         gTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGt                       <  2:1499272/76‑1 (MQ=255)                                          tAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTg                      <  2:892498/76‑1 (MQ=255)                                           aaTACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGa                     <  2:716400/76‑1 (MQ=255)                                           aaTACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGa                     >  1:1074675/1‑76 (MQ=255)                                            aTACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGaa                    <  2:1652341/76‑1 (MQ=255)                                             tACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAg                   >  2:1260310/1‑76 (MQ=255)                                              aCCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGc                  >  2:538796/1‑76 (MQ=255)                                                 acaACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGcaca               >  2:165353/1‑76 (MQ=255)                                                 aanaCCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGcaca               >  2:1591512/4‑76 (MQ=255)                                                  caACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAg              >  2:102400/1‑76 (MQ=255)                                                   aaCCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGt             >  2:1013816/1‑76 (MQ=255)                                                    aCCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTc            <  1:1719592/76‑1 (MQ=255)                                                     cccTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCg           >  1:1720346/1‑76 (MQ=255)                                                       cTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCgtg         <  1:1973029/76‑1 (MQ=255)                                                       cTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCgtg         >  1:1592994/1‑76 (MQ=255)                                                        ttAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCgtgt        >  2:1801166/1‑76 (MQ=255)                                                          aaGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCa      <  1:200065/76‑1 (MQ=255)                                                          aaGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCa      <  1:1982588/76‑1 (MQ=255)                                                          aaGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCa      <  1:1676167/76‑1 (MQ=255)                                                          aaGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCa      >  1:802643/1‑76 (MQ=255)                                                             ttAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCt   <  1:835434/76‑1 (MQ=255)                                                             ttAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCt   <  1:1580492/76‑1 (MQ=255)                                                             ttAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCt   >  2:1175448/1‑76 (MQ=255)                                                              tAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTg  <  1:511875/76‑1 (MQ=255)                                                              tAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTg  >  1:848136/1‑76 (MQ=255)                                                              tAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTg  <  1:1552148/76‑1 (MQ=255)                                                                 |                                                                         TCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTG  >  REL606/430771‑430907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑