| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,161,145 | G→T | R97L (CGC→CTC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,161,145 | 0 | G | T | 97.5% | 147.4 / ‑2.4 | 40 | R97L (CGC→CTC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (18/21); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
TACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAGGCACCGTAAAAGACATTAAAGAACAAGA > REL606/4161072‑4161217 | tACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGctcg > 1:1008409/1‑76 (MQ=255) aCCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGt < 1:1139044/76‑1 (MQ=255) aCCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGt < 1:1591299/76‑1 (MQ=255) ccGCCTTGGCGAGCCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGtt < 1:1045978/76‑1 (MQ=255) ccGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGtt < 2:1089496/76‑1 (MQ=255) cGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTa < 1:228438/76‑1 (MQ=255) ccTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTaaa < 2:432921/76‑1 (MQ=255) ttGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACt < 1:2170395/76‑1 (MQ=255) tGGCGAACCGGTGTTTGACGTCCAGGGATGTAAAATCCGTGGCGTGACCTGTTCCGCACCGCTGCTCGTTAAACTg > 1:1897964/1‑76 (MQ=255) tGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTg > 2:1907583/1‑76 (MQ=255) cGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGt < 1:900818/76‑1 (MQ=255) gAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTc > 2:1060654/1‑76 (MQ=255) aaCCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCGGCTCGTTAAACAGCgcgg > 2:76438/1‑73 (MQ=255) tgtTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCCCCGCTGCTCGTTAAACTGCGTCTGGTGAt < 1:2018652/76‑1 (MQ=255) gtTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTGGTGATc > 2:8491/1‑76 (MQ=255) ttGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTa < 1:1880021/76‑1 (MQ=255) tGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTAt > 1:968757/1‑76 (MQ=255) tGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTAt > 1:2166009/1‑76 (MQ=255) aCGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGa > 1:243378/1‑76 (MQ=255) aCGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGa < 1:1701882/76‑1 (MQ=255) ccAGGAATGTCAAATCCGTCGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAgcgc < 2:1562849/76‑1 (MQ=255) cAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGcgcg < 1:1983223/76‑1 (MQ=255) aGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGa > 1:448142/1‑76 (MQ=255) aGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGa > 2:123066/1‑76 (MQ=255) ggAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGaa < 1:272082/76‑1 (MQ=255) tCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGATGCGCCg < 2:1771686/76‑1 (MQ=255) tCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCg < 2:743466/76‑1 (MQ=255) tCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCg < 2:1004013/76‑1 (MQ=255) aaaTCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGa < 2:467137/76‑1 (MQ=255) aaaTCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGa > 1:2098951/1‑76 (MQ=255) aaTCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGaa > 1:533647/1‑76 (MQ=255) aTCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATTTATGAGCGCGAAGCGCCGGAAg > 2:91964/1‑76 (MQ=255) aTCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAg > 1:164837/1‑76 (MQ=255) tCCGTGGCGTGCCCTATTCCGCCCCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAgg < 2:1555553/76‑1 (MQ=255) tCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAgg > 2:1901982/1‑76 (MQ=255) tCCGTGGCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAgg < 2:261674/76‑1 (MQ=255) ggCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAGGCACCg < 2:140379/76‑1 (MQ=255) gCGTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAGGCACCGt > 1:1437366/1‑76 (MQ=255) ggTGACCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTAGGAGCGCGAAGCGCCGGAAGGCACCGTa < 2:171567/75‑1 (MQ=255) cGTGCCCTATTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAGGCACCGTa < 2:698811/76‑1 (MQ=255) aCCTATTCCGCGCCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAGGCACCGTAAAAg < 2:1850206/76‑1 (MQ=255) aTTCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAGGCACCGTAAAAGACAt > 1:780540/1‑76 (MQ=255) ttCCGCACCGCTGCTCGTTAAACTGCGTCTGGTAATCTATGAGCGCGAAGCGCCGGAAGGCACCGTAAAAGACAtt < 2:2192739/76‑1 (MQ=255) tCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAGGCACCGTAAAAGACATTa > 2:1209776/1‑76 (MQ=255) tCCGCACCGCTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAGGCACCGTAAAAGACATTa > 1:1461041/1‑76 (MQ=255) ccGCCCCGCTGCTCTTTAAACTGCGTTTGGTGATCTAAGAGCGCGAGGCGCCGGAAGGCACCGTAAAAGACATTaa < 1:2104163/76‑1 (MQ=255) ccGCTGCTCTTAAACCGGCGTGGGGTGATTTGGGGGCGGGAGGCCCCGGAAGGCACCATAAAAGACATTAAAGAAc < 1:338717/76‑1 (MQ=255) cTGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAGGCACCGTAAAAGACATTAAAGAACaag > 2:1959666/1‑76 (MQ=255) tGCTCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAGGCACCGTAAAAGACATTAAAGAACaaga > 1:1570307/1‑76 (MQ=255) tGCTCGTTAAACGGCGTCTGGTGATCTAGGAGCGCGAAGCGCCGGAAGGCACCGTAAAAGACATTAAAGAACaaga < 2:65999/76‑1 (MQ=255) | TACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTGGTGATCTATGAGCGCGAAGCGCCGGAAGGCACCGTAAAAGACATTAAAGAACAAGA > REL606/4161072‑4161217 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |