| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,471,518 | T→A | I325N (ATC→AAC) | yjgQ → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,471,518 | 0 | T | A | 98.4% | 227.6 / NA | 61 | I325N (ATC→AAC) | yjgQ | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (44/16); minor base C (0/1); total (44/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TAGCGTACCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTT > REL606/4471447‑4471590 | tagcgtaCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTc > 1:2112048/1‑76 (MQ=255) agcgtaCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCg < 1:282006/76‑1 (MQ=255) cgtaCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGc > 1:318235/1‑76 (MQ=255) gtaCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGcc > 2:1894739/1‑76 (MQ=255) taCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTTTCTTCTACGTACTGGACCAGAACTTCCGccc > 1:617325/1‑76 (MQ=255) taCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAAATTCGGccc > 1:1974562/1‑76 (MQ=255) aCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGcccc > 1:1381032/1‑75 (MQ=255) aCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCg < 1:593291/76‑1 (MQ=255) cGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCt > 1:3960/1‑76 (MQ=255) aTGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGa > 1:1962390/1‑76 (MQ=255) tGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCCCTGAc > 2:1118043/1‑76 (MQ=255) gcgtgcgtGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGtt > 1:2136934/1‑76 (MQ=255) gcgtgTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGtt > 2:995882/1‑76 (MQ=255) gcgtgTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGtt < 2:536516/76‑1 (MQ=255) gtgtGGTCACCGGTATCAGTTTCGGTTTTTTCTTCTACGTACTGGACCAGAACTTCGGCCCGCCGCCGTTGGTTTa > 1:987330/1‑76 (MQ=255) gtgtGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTa < 2:1969631/76‑1 (MQ=255) gtgtGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTa > 2:1505171/1‑76 (MQ=255) tgtgGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTAt > 1:100401/1‑76 (MQ=255) gtgGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCCGACGTTGGTTTTTg > 1:2169349/1‑76 (MQ=255) ggTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGc > 1:2106409/1‑76 (MQ=255) gacACCGGTATCAATTTCGGTGTTCTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCa < 1:1283817/74‑1 (MQ=255) gTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCCGACGTTGGTTTATGGCa > 1:207970/1‑76 (MQ=255) cACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATc < 2:775496/76‑1 (MQ=255) aCCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATcc > 2:501306/1‑76 (MQ=255) aCCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATcc < 2:1803225/76‑1 (MQ=255) cgttATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCccg < 2:231274/73‑1 (MQ=255) cGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTTTGGCATcccc > 1:1068682/1‑75 (MQ=255) cGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCccg > 1:64127/1‑76 (MQ=255) tATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCccgccg > 1:644644/1‑76 (MQ=255) tATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCCGACGTTGGTTTATGGCATCcccccg > 1:1891981/1‑76 (MQ=255) aGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGatca < 2:1954875/76‑1 (MQ=255) gTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGatcat > 2:164185/1‑76 (MQ=255) tttCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGgtcatc > 1:114941/1‑76 (MQ=255) ttCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGGTCATCg > 1:532345/1‑76 (MQ=255) ttCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGCTCATCg > 1:957336/1‑76 (MQ=255) ggTTTTTTCTTCTCCGTACTGGACCAGAACTTCGGCCCGCTGTCGTTGGTTTATGGCATCCCGCCGATCATCGgcg < 1:404898/76‑1 (MQ=255) ggTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGgcg > 2:364738/1‑76 (MQ=255) ggTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGgcg > 1:566825/1‑76 (MQ=255) ggTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGgcg > 1:1736002/1‑76 (MQ=255) ggTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGgcg > 1:2059855/1‑76 (MQ=255) ttttGTCTTCTACGTACTGGACCAGACCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCa < 2:1340342/76‑1 (MQ=255) ttttGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGgcgcc > 1:279218/1‑75 (MQ=255) ttttGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCa > 1:1839336/1‑76 (MQ=255) ttGTCTTCTACCTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACt > 1:781991/1‑76 (MQ=255) tGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTg > 1:1023732/1‑76 (MQ=255) gTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGt > 1:1823432/1‑76 (MQ=255) gTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGt > 1:2112853/1‑76 (MQ=255) gTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGt > 1:2153001/1‑76 (MQ=255) tcttctACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGtt < 1:1802507/76‑1 (MQ=255) tctACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGcc > 1:10469/1‑76 (MQ=255) tctACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGcc > 2:870503/1‑76 (MQ=255) ctACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCa < 2:200484/76‑1 (MQ=255) tACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCaa > 1:1957217/1‑76 (MQ=255) aCGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAg > 1:1785512/1‑76 (MQ=255) gTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAgcg > 2:1930576/1‑76 (MQ=255) gTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAgcg < 1:833128/76‑1 (MQ=255) tACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCCAgcgc > 1:1215886/1‑76 (MQ=255) aCTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGcc < 2:1035709/76‑1 (MQ=255) cTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCa < 1:2119082/76‑1 (MQ=255) tGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAg > 1:1294491/1‑76 (MQ=255) tGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCCGCGCACTGTTGCCAAGCGCCAg > 1:865043/1‑76 (MQ=255) ccAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGcttc < 2:2139497/76‑1 (MQ=255) ccAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGcttc < 2:69525/76‑1 (MQ=255) ccAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCCGCGCACTGTTGCCCAGCGCCAGcttc > 2:931721/1‑76 (MQ=255) cAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGcttct > 2:1189524/1‑76 (MQ=255) cAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGcttct < 1:648533/76‑1 (MQ=255) aGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGcttctt > 1:61378/1‑76 (MQ=255) aGAACTTCGGCCCGCTGACGTTAGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGcttctt > 1:2219852/1‑76 (MQ=255) | TAGCGTACCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTT > REL606/4471447‑4471590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |