BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,305,551	A→T	I230N (ATC→AAC)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,305,551	0	A	T	100.0%	230.6 / NA	57	I230N (ATC→AAC)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (26/31); total (26/31)

TACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGG  >  REL606/1305478‑1305623

tACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgttc                                                                        <  2:1064758/76‑1 (MQ=255)

  cGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgttcat                                                                      <  1:2236826/76‑1 (MQ=255)

  cGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgttcat                                                                      >  1:1466057/1‑76 (MQ=255)

    cccGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgttcatga                                                                    >  2:1566660/1‑76 (MQ=255)

     ccGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgttcatgat                                                                   <  2:1823443/76‑1 (MQ=255)

      cGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgttcatgatc                                                                  <  1:1238234/76‑1 (MQ=255)

       gCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgttcatgatca                                                                 <  2:915355/76‑1 (MQ=255)

        cATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgttcatgatcat                                                                <  2:1487557/76‑1 (MQ=255)

         aTCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGatcatc                                                               >  1:1584933/1‑76 (MQ=255)

          tCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCt                                                              <  1:1411648/76‑1 (MQ=255)

            ttGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTg                                                            >  2:2040526/1‑76 (MQ=255)

            ttGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTg                                                            >  1:1487064/1‑76 (MQ=255)

             tGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGc                                                           <  2:1209610/76‑1 (MQ=255)

               tttGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGa                                                         >  1:321265/1‑76 (MQ=255)

                 tGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATg                                                       >  1:420274/1‑76 (MQ=255)

                 tGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATg                                                       >  1:2041979/1‑76 (MQ=255)

                   aaGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGtt                                                     <  2:995155/76‑1 (MQ=255)

                     gTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTgg                                                   <  2:545225/76‑1 (MQ=255)

                      tAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGgc                                                  >  1:1839152/1‑76 (MQ=255)

                       aGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGGTGTTGgcg                                                 >  2:1359524/1‑76 (MQ=255)

                        gCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGgcgc                                                <  2:1871652/76‑1 (MQ=255)

                          gAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAg                                              >  2:300511/1‑76 (MQ=255)

                              ttCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTcc                                          <  2:515481/75‑1 (MQ=255)

                              gTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTcc                                          <  2:1490914/76‑1 (MQ=255)

                              gTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTcc                                          <  1:691849/76‑1 (MQ=255)

                                cGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCAt                                        <  1:1109762/76‑1 (MQ=255)

                                 gACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATa                                       <  1:793835/76‑1 (MQ=255)

                                 gACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATa                                       >  2:329032/1‑76 (MQ=255)

                                  aCCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATAc                                      >  1:108902/1‑76 (MQ=255)

                                  aCCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATAc                                      <  2:1639238/76‑1 (MQ=255)

                                    cATATTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACgg                                    >  2:537906/1‑76 (MQ=255)

                                     atatTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGc                                   <  1:1615483/76‑1 (MQ=255)

                                      tatTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCg                                  >  1:780707/1‑76 (MQ=255)

                                       atTCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGt                                 <  1:322833/76‑1 (MQ=255)

                                        ttCATGCTGCCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTa                                <  2:601817/76‑1 (MQ=255)

                                            tgctgcCGGTGGACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCTATACGGCGTagaaa                            >  2:1206816/1‑76 (MQ=255)

                                             gctgcCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAac                           <  1:2234403/76‑1 (MQ=255)

                                               tgcCGGTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAacac                         >  1:2087082/1‑76 (MQ=255)

                                                   ggTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGc                     <  2:1040013/76‑1 (MQ=255)

                                                   ggTGTACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGc                     >  2:2240108/1‑76 (MQ=255)

                                                    gtgtACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCa                    >  2:384149/1‑76 (MQ=255)

                                                    gtgtACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCa                    >  2:999705/1‑76 (MQ=255)

                                                     tgtACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCAt                   <  1:1396591/76‑1 (MQ=255)

                                                      gtACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATc                  >  1:673628/1‑76 (MQ=255)

                                                      gtACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATc                  <  2:133123/76‑1 (MQ=255)

                                                       tACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCa                 >  2:1753421/1‑76 (MQ=255)

                                                       tACGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCa                 <  1:695128/76‑1 (MQ=255)

                                                        aCGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAg                <  1:413046/76‑1 (MQ=255)

                                                        aCGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAg                <  1:1811000/76‑1 (MQ=255)

                                                        aCGCGATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAg                >  1:12303/1‑76 (MQ=255)

                                                            gATGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTg            <  1:1494403/76‑1 (MQ=255)

                                                             aTGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGa           <  1:1123279/76‑1 (MQ=255)

                                                              tGTAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGAc          <  1:1167560/76‑1 (MQ=255)

                                                                tAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCt        >  2:1550867/1‑76 (MQ=255)

                                                                tAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCt        <  1:1214475/76‑1 (MQ=255)

                                                                tAATTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCt        <  1:1471100/76‑1 (MQ=255)

                                                                 aaTTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCtt       >  1:2238770/1‑76 (MQ=255)

                                                                 aaTTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCtt       <  1:217525/76‑1 (MQ=255)

                                                                  aTTATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCttt      <  2:813333/76‑1 (MQ=255)

                                                                    tATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTaa    <  1:1094592/76‑1 (MQ=255)

                                                                    tATCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTaa    <  2:368755/76‑1 (MQ=255)

                                                                     aTCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAg   >  1:2142488/1‑76 (MQ=255)

                                                                     aTCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAg   <  2:2124567/76‑1 (MQ=255)

                                                                      tCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCATATTGACCTTTAAgg  >  1:626134/1‑76 (MQ=255)

                                                                      tCGTTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAgg  >  1:915808/1‑76 (MQ=255)

TACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGG  >  REL606/1305478‑1305623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`